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伤寒杆菌

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Objective To study the conjugational transfer and antibiotic resistance expression of pRST98 in different enteric bacilli(including E.coli, S.typhi, S.typhimurium and S.flexneri).

目的 研究伤寒杆菌耐药质粒pRST98在不同种属肠道杆菌间(大肠杆菌、伤寒杆菌、鼠伤寒杆菌及痢疾杆菌)互相接合传递的规律及表达。

Both 50% boiling water extract and 50% alcohol extract of Zingiber Coral- linum Hance do not inhibtte the five tested bactreial species:Staphy.aureus, Strept.hemolytieus,B.subtilis,E.coli and S.typhus.

本文报告对珊瑚姜抑制细菌和真菌作用的研究。结果表明珊瑚姜50%水煎剂和50%酒精浸液对金黄色葡萄球菌、乙型溶血性链球菌、枯草杆菌、大肠杆菌和伤寒杆菌都无抑制作用。

Objective To determine the invasive ability of recombinant attenuated Salmonella typhimurium X4064(pQEM1)strain containing gene fragment of Plasmodium falciparium merozoite surface protein 1 (MSP1),the stability of its plasmid, and its re-expression.

目的 检测含恶性疟原虫裂殖子表面蛋白1基因片断的重组减毒鼠伤寒杆菌X4064(pQEM1)的入侵宿主能力,所含质粒的稳定性和再表达能力。

The ferment product of strains JF and Al with a broad spectrum of antimicrobial activity, against one or more sensitive microbes, such as Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli and Typhoid bacillus etc, were screened out using the filter paper assay, strain C6 have inhibiting effects on VSMC proliferation.

结果:从槲寄生中分离到JF、A1、G6等11株内生真菌;G6对牛主动脉平滑肌细胞增殖有抑制作用;JF、A1发酵产物具有较广语的抗菌活性,对革兰氏阳性细菌如表皮葡萄球菌,革兰氏阴性细菌如产气肠杆菌、大肠杆菌、伤寒杆菌等均具有较强的抑制作用。

Abjective to express the Hepatitis A virus genome cDNA attenuated salmonella Typhimurium and to find the immunity to hepatitis A virus after administrated that salmonella Typhimurium with hepatitis A virus gemome to the mousehepatitis A virus coding region cDNA were inserted expressive plasmid PBV221 and then were transform into E coli DH5α.

使甲型肝炎病毒基因组cDNA能够在减毒伤寒杆菌中表达,从而通过口服此种减毒伤寒杆菌达到对甲肝免疫的作用。将甲肝病毒编码区cDNA插入高效表达质粒PBV221,转化大肠杆菌DH5α,筛选并鉴定阳性克隆。

Methods:Widal test was analysed retrospectively in 87 bacteriologically proven cases of typhoid fever and 922 patients with non typhoidal fever.

对251例伤寒患儿中伤寒杆菌培养阳性的87例患儿及922例非伤寒发热患儿的肥达反应结果进行回顾性分析。

Coli, Shigella sonnei s, Proteus, typhoid and vice Salmonella typhi, Pseudomonas aeruginosa, Vibrio cholerae and other enteric pathogens are effective; its ethanol extract on the number of gram-positive and-negative bacteria, and human-type Mycobacterium significant antibacterial activity; ebony decoction on in vitro to be sore dermatophytes, Epidermophyton floccosum, plaster-like pathogenic microorganisms in the skin of small spores of fungi

广谱抗菌:实验证明,乌梅水煎液对炭疽杆菌、白喉和类白喉杆菌、葡萄球菌、肺炎球菌等皆有抑制作用,对大肠杆菌、宋内氏痢疾杆菌、变形杆菌、伤寒和副伤寒杆菌、绿脓杆菌、霍乱弧菌等肠内致病菌也有效;其乙醇浸液对一些革兰阳性和阴性细菌,及人型结核杆菌均有显著抗菌作用;乌梅水煎液在试管内对须疮癣菌、絮状表皮癣菌、石膏样小芽孢菌等致病皮肤真菌有抑制作用

There are high level of specific IgG and IgA against Fe-SOD in sera and lavage of intestines in mice intranasally administrated with S. typhi Fe-SOD, but there are high level of specific IgG in sera only in mice immunized with S. typhi Fe-SOD peritoneally.

结果 当用IL-1作为佐剂时,经鼻腔接种伤寒杆菌Fe-SOD的小鼠血液和肠液中可产生高水平特异性IgG和IgA;而腹腔注射仅在血液中产生高水平特异性IgG。

Male Wistar rats were given intrajejunally the live culture of Salmonella typhi [1x1010 colony-forming unit/rat] and followed by deprivation of food for 36 h.

雄性 Wistar 大白鼠经由空肠投与鼠伤寒杆菌菌株(1 x1010 CFU/rat)后,绝食 36 小时。

The recombinant plasmid pQEM1 could steadily exist in the X4064 strain of S. typhimurium,without influence on its invasion into host cells.X4060(pQEM1)strain isolated from infected mice still had the ability to re-express M1 protein.

重组pQEM1质粒能稳定地存在于减毒鼠伤寒杆菌X4064株中,不妨碍其入侵宿主细胞,从小鼠组织中分离到的X4064(pQEM1)依然具有表达M1蛋白的能力。

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