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伞形花内酯

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In this work, the interaction between umbelliferone and bovine serum albumin was studied by performing two different kinds of titration modes. The two spectroscopic data matrices were augmented, and then the information of experiment data was increased. The augmented experiment data matrix was analyzed by the MCR-ALS method. This procedure allowed the recovery of the concentration profiles for each species and the calculation of the ∶ ratio in the complex and the apparent equilibrium constant.

采用两种不同的试剂滴加模式对伞形花内酯与BSA的相互作用进行研究,对经典的荧光光谱数据矩阵加以扩展,增加了实验数据的信息量;进而应用MCR-ALS对该扩展的荧光光谱矩阵进行迭代计算,较好地分辨出作用过程中浓度变化趋势图,并计算出伞形花内酯与BSA的表观结合常数和结合比。

Results: It showed that esculin, demethylfuropinarine, decursin and columbianadin inhibited growth of human carcinoma cell line KB cell and their activity showed doseeffect relationship; and umbelliferone renecioate, sphondin and coumestrol inhibited growth of human leukemia cell line HL60 cell and their activity showed doseeffect relationship.

结果:马栗树皮苷、去甲基呋喃皮纳灵、前胡素和二氢山芹醇当归酸酯对人鼻咽癌细胞株KB细胞的生长有一定程度的抑制作用,且呈浓度-效应关系;伞形花内酯刘寄奴酸酯、牛防风素和考迈斯托醇对人白血病细胞株HL-60细胞的生长有一定程度的抑制作用。

Esculin, demethylfuropinarine, decursin and columbianadin inhibited growth of human carcinoma cell line KB cell. Umbelliferone renecioate, sphondin and coumestrol inhibited growth of human leukemia cell line HL60 cell.

马栗树皮苷、去甲基呋喃皮纳灵、前胡素和二氢山芹醇当归酸酯对人鼻咽癌细胞株KB细胞的生长有抑制作用;伞形花内酯刘寄奴酸酯、牛防风素和考迈斯托醇对人白血病细胞株HL-60细胞的生长有抑制作用。

Condensation of resorcinol or phloroglucinol with sodium hydrogen malate in the presence of catalytic sulfuric acid leads to the synthesis of natural bioactive umbelliferone and daphnetin under microwave irradiation.

在适量硫酸的存在下,用微波辐射合成了天然活性香豆素伞形花内酯和瑞香内酯。

The effect of umbelliferone on the conformation of BSA was also analyzed via synchronous fluorescence spectrometry.

通过同步荧光光谱法发现伞形花内酯对BSA的构象有一定的影响。

RESULTS & CONCLUSIONS: 8 compounds were isolated and 6 compounds elucidated as Bergarpten, Umbelliferone, Skimmianine, Kaempferol, 3,5 - Diacetyltambulin and Zanthonitrile.

结果与结论:从竹叶椒果实的脂溶性部位分离得到了8个化合物,鉴定了其中的6个化合物,分别是化合物1香柑内酯、化合物2伞形花内酯、化合物3菌芋碱、化合物4山奈酚、化合物5(3,5-Diacetyltambulin)、化合物6;其中山奈酚系首次从花椒属植物中分离得到。

Under the imitated physiological conditions(pH=7.4), the interaction between umbelliferone and bovine serum albumin was investigated by the fluorescence spectroscopy and the multivariate curve resolution-alternating least squares method.

在模拟人体生理条件下(pH=7.4),用荧光光谱法结合多元曲线分辨-交替最小二乘法研究伞形花内酯与牛血清白蛋白的相互作用。

Seven chemical constituents were identified on the basis of chemical methods and spectral evidences. ResultsFrom this plant, seven active compounds were isolated. They were determined as farrerol, myricetin ,quercetin, taxifolin, umbelliferone, hyperin and juniper camphor.

结果从满山红中分离得到了7种活性化合物,分别为杜鹃素,杨梅素,槲皮素,二氢槲皮素,伞形花内酯,金丝桃苷,杜松脑。

The absorption and transport of umbelliferone, osthole, columbianadin, columbianetin acetate, angelolA and angelolB are passive diffusion as the dominating process in Caco2 cell monolayer model.

为了考察独活中香豆素类化学成分在整体口服实验中能否被吸收和吸收程度,本文首先采用Caco2细胞单层模型[8]研究独活中的主要化学成分伞形花内酯、甲氧基欧芹素、二氢欧山芹素、二氢欧山芹醇乙酯、当归醇A和当归醇B的吸收和转运。6种香豆素类成分的化学结构见图1。

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