以...命名

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Since the second half of this year, is Haoxiliantai, keep the good news: in Loudi organized by the children's song and dance competitions in the province, Zhangjiajie was one of the two gold silver and one bronze; held in Suzhou in the first national farmers will turn in literature and art, Sangzhi Lantern,"Yang Spring bird reminder "was third, 6 for the province's award-winning one; held in Changsha, Hunan harmony in the United States and the text reading contest, Zhangjiajie was the Gold Award, the Urban Culture Award by the organization; At the same time, also successfully held Zhangjiajie sing the "eco-tourism Cup" Xiang Xiang Song of the trials and Sangzhi China Minge Jie, Sangzhi County, was named the country's rural Chinese folk ......

今年下半年以来，可谓好戏连台，喜讯不断：在娄底举办的全省少儿歌舞大赛中，张家界获得一金两银一铜；在苏州举办的全国首届农民文艺会演中，桑植花灯《阳雀催春》获三等奖，此为全省6个获奖项目之一；在长沙举办的和谐湖南美文诵读大赛中，张家界获金奖，市文化局获组织工作奖；同时，还成功举办了唱响张家界&生态旅游杯&湘人湘歌选拔赛和中国桑植民歌节，桑植县被国家命名为中国民歌之乡……这是我市文化战线坚持科学发展观，解放思想，在对待非物质文化遗产上走以申报求保护、以传承求发展的新路子取得的令人瞩目的成果。

Muscular and handsome breed of working dog known for its distinctive white mask and dark widow's peak; also called Arctic sled dog; double coat is thick, of medium length, coarse, and insular and comes in various shades of gray with lighter white and darker black areas; ears relatively small compared to head and are erect and slightly rounded at ends; tail is bushy and carried gaily and loosely curled over rump; eyes are almond-shaped, wolflike yet trusting, and light brown in color; adult stands 20 25 in.(51 64 cm) tall at shoulders and weighs 50 85 lbs (23 39 kg); firm, compact feet allow dog to pull heavy loads through deep snow; where the Siberian husky is designed for speed, this breed is designed for power; very fond of people, especially children; enjoys rolling around in snow and prefers outdoor life; great at sled-pulling contests; named after the Mahlemuts, nomadic and skilled Inuit people who migrated from Siberia over a land bridge into northwestern Alaska with these dogs thousands of years ago.

爱斯基摩犬是工作犬的一支，肌肉发达，英俊潇洒，因其与众不同的白色双颊和暗色额头&V&型发尖而闻名，它也被称为&北极雪橇犬&；厚而粗糙适中长度偏狭的双层毛皮，以多样化的灰度梯度混杂着轻度的白色和深黑区域表现出来；耳朵相对头部较小，挺立并轻微地环绕在头部末位；尾巴浓密的，松弛地卷曲盘绕在臀部上方；眼睛呈杏仁状，狼一般的长相，值得信赖，毛色呈浅棕色；成年犬站立肩高51—64厘米，重23—39公斤；牢固坚实的足部使得爱斯基摩犬能够拉动重负载在深厚的雪中前进；如果说西伯利亚哈士奇被认为是速度的象征，爱斯基摩犬被认为是力量的象征；它们喜欢人类，尤其是孩子，喜欢在雪中滚动，尤其喜欢户外生活；非常擅长牵引雪橇的竞赛；它以爱斯基摩而命名，游牧和富有经验的因纽特人在几千年前和这些狗一起从西伯利亚通过北方大陆桥迁移到阿拉斯加。

According to the Pyongyang Times, cultivators 'have ensured the right temperature in day and at night and prevented damage from blights ...

节日组委会向全国各地的温室提供了通过组织培养方法繁育的种子，以增加&金日成花&的种植，这种杂交兰花以金日成的名字命名。

You have the wild plant to have 1,452 kinds, in which area national kind of protection to have the metasequoia, two kind ofprotections has the gingko and so on 4 kinds, three kind ofprotections 8 kinds, have the species which 石斛 and so on 1Ospecies is borders on exterminates, 6 kinds for the Chinese uniquekind, the Huangshan Mountain unique 2 kinds, the initial contact orthe plant which to Huangshan Mountain names have 28 kinds, You Yimingthe tea "the Huangshan Mountain Mao fong", the famous medicine "theHuangshan Mountain ganoderma lucidum" are renowned at home and abroad.

你有野生植物有1452种，其中属国家一类保护的有水杉，二类保护的有银杏等4种，三类保护的8种，有石斛等1O个物种属濒临灭绝的物种，6种为中国特有种，黄山特有2种，首次发现或以黄山命名的植物有28种，尤以名茶&黄山毛峰&、名药&黄山灵芝&驰名中外。

In particular, with the help of the program it is possible to duplicate path into the buffer of exchange, to check and to change the expansion of file, change date and attributes of file, open command line, rename files in the batch mode, find and to replace the text in large quantities of files, to synchronize contents of files, to be switched between the frequently utilized files and much more.

特别是，借助这一计划是有可能重复路径进入缓冲区的交流，以检查和改变扩展文件，更改日期及属性的文件，打开命令行，重命名文件，在批处理模式，找到并案文取代大量的档案，以同步的档案内容，将开关之间经常利用档案，甚至还有更多。

As a kind of chocolate, The Godiva which was originated from Brussels , Belgium and was founded by Mr. Joseph Draps, a chocolate great master has the history of more than 175 years. It was said it named from Mrs. Godiva and is famous for its grace fragrant and taste.

起源于比利时布鲁塞尔的 Godiva ，由巧克力大师 Joseph Draps 一手创办，并以传说中尊贵的 Godiva 夫人命名，至今已超过175年历史，以优雅的香味和口感著称于世。

The two most common ways to synchronize to Flash content are to navigate to frames containing frame labels that match the name of the cue point (in an ActionScript 2.0 or ActionScript 3.0 timeline-based file) or to navigate to slides named the same as the cue point names (in an ActionScript 2.0 screens-based file).

这两个最常见的方式进行同步，以Flash内容是浏览含有帧帧标签的名称相匹配的提示点（在一个动作2.0或3.0的动作时间为基础的档案），或以浏览幻灯片命名一样线索点的名称（在一个动作2.0屏幕的文件）。

As a specific social group in ancient China, recluses are entitled in five categories according to the following: the description of them as men; their reclusion action; the prototype and derivation of them; their residence space and classical literature about reclusion.

隐士是中国古代社会的一个特殊群体，隐士的称谓多种多样，按照其命名方式，大致可概括为五类：以&人&为描述中心的称谓；隐逸行动作为身份指示的称谓；隐士原型及其衍生的称谓；以隐士居处空间作为指代的称谓；来自经典隐士文本的称谓。

RosettaNet is named after the Rosetta Stone, which, carved with the same message in three languages, led to the understanding of hieroglyphics.

RosettaNet 命名以Rosetta 石头，哪些，雕刻以同样消息在三种语言，导致对hieroglyphics 的理解。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。