交叉反应

Owing to the variability of HVR1, the method to check anti-HVR1 antibodies need to cloning and expressing the gene of HVR1 of every sera and that poses some problem. For example, it needs lots of time and money, and the sample must come from viremic patients. It is urgent to acquire a broadly cross-reactivity HVR1 antigen to check the antiHVR1 Ab in the sera of HCV infected patients by ELISA.

由于HVR1的高变性，因此要想测定血清中抗HVR1水平，通常要克隆表达待测血清中的HVR1基因，但该方法时间长、费用高，并仅对HCV、RNA阳性血清适用，所以急需一种具有极高交叉反应性的HVR1抗原，才可通过ELISA对病人的HVR1抗体进行检测。

Langone JJ (1982) Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumococci.

其中，醛基免疫磁性载体的捕获和分离效果最佳，且用乙醛—右旋糖苷进行包被可以消除交叉反应。

Titers and affinity constant of McAb were determined with indirect ELISA, and cross reactivity of McAb with indirect competitive ELISA.

用间接ELISA检测该McAb的效价和亲和性，用间接竞争ELISA检测与其他结构类似物的交叉反应率。

The cross reactivity with other SAs was less than 1×10~(-3)%. The affinity constant of the anti-SQ monoclonal antibody SQ_(12)A_(10) was 1.40×10~9L/mol.

经鉴定，三株单抗与其他八种磺胺类药物交叉反应率＜1×10~(-3)％，其中SQ_(12)A_(10)单克隆抗体亲和常数为1.40×10~9L／mol，。

The relative cross-reactivity of PA with AFB-1,ZON,T-2,FB were less than 0.01%and with an affinity constant for PA of about 1.54×10~8 liters per mol.It offered a foundation for developing immunoassay method for PA.

单克隆抗体与黄曲霉毒素B1、玉米赤霉烯酮、T-2毒素和伏马毒素B1均无交叉反应率，单抗的亲和常数为1.54x10~8 L/mol，为进一步建立青霉酸的免疫学快速检测方法提供了基础。3。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

Results: The two rabbit antisera had a title of 1:20,000 and 1:200,000, respectively, and had good cross-reactions with manmade human PC-1 protein N terminal 46 amino acids.

结果：两个兔多克隆血清的效价分别为1：20，000和1：200，000，并能与人工合成的人PC-1蛋白N端的46个氨基酸发生良好的交叉反应。

Convincing evidence indicates that matching for HLA class I cross-reactive groups rather than conventional HLA antigen matching may result in improved renal graft survival. T and B lymphocyte crossmatch before transplantation was done to determine whether there is any human leukocyte antigen antibodies to the graft.

等待肾脏移植的病人数量有限，然而要找到HLA配对很好的机率真的很低，后来有新的策略，将大量的HLA等位基因缩减为一些决定常见的HLA抗原的少量联系紧密的HLA等位基因组，称为交叉反应组。

Results 371 bp DNA fragment was amplified from 24 different species. The sensitivity could be improved to 10-12 g. No signal was observed when human DNA,funguses and viruses were used as templates. 22 blood samples and 4 cerebrospinal fluid samples, being positive with culture, were positive by using PCR. The gramnegative and grampositive probes hybridized to clinic samples and different species, as predicted by Gram stain characteristics.

结果 对24株不同标准菌株进行PCR扩增，均出现371 bp长度的DNA片段，敏感性试验可检测出10-12 g的细菌DNA，与人类基因组DNA、真菌及病毒无交叉反应；22例血培养阳性标本及4例脑脊液培养阳性标本均扩增出371 bp长度DNA条带，反相杂交法区分革兰阳性/阴性细菌与培养结果相符。

In a third reaction the amount of bound detecting antibody in the coating antibody-proinsulin-detection antibody complex is visualised by use of streptavidin-peroxidase that binds to the biotin label.

该方法的灵敏度为0.6306miu/L，与142pmol/L的胰岛素原、3960pmol/L的C肽无交叉反应，平均回收率为97.12％，批内与批间变异系数分别为11.65％与5.95％，线性标准范围为0.63～100miu/L。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。