乌头碱

Objective: To study the effect of total alkaloides of aconitum excelsum reichb on CYP2E1 metabolism activity in morphine dependent rats.

目的：观察紫花高乌头总碱对吗啡依赖大鼠CYP2E1代谢活力的影响。

Result: HPLC-MS analysis shows that the major hydrolysis products are benzoylaconine and aconine.

结果：经HPLC-MS分析，鉴定主要水解成分峰为苯甲酰乌头原碱和乌头原碱。

We use Bond Elute C18 solid-phase extraction cartridge, the hope was isolated from the urine of rabbits administered the pre-contracting out of aconitine aconitine times the original base Aconine and alkali Benzoylaconine other metabolites, the methanol elution phase, but it does not Er Shi found that the chemical is expected to experiment an impasse.

我们利用Bond Elute C18固相萃取分离小柱，希望从给药家兔尿液中分离出预判的乌头原碱Aconine和乌头次碱Benzoylaconine等代谢产物时，甲醇洗脱相中却并没有发现预期的化学物质而使实验陷入了僵局。

RESULT ①Two different ITS2 sequences (GenBank accession number: AF416783, AF416784) and D3 sequences (AF416782, AF415594), named as An. minimus A and An. minimus C, were detected in this study. They differed by 5. 8％ in the ITS2 region with 22 fixed nucleotide substitutions and indels, and by 1. 5％ in D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An.

结果 ①分别发现两种不同的ITS2序列（GeneBank登录号：AF416783，AF416784）和D3序列（GeneBank登录号：AF416782，AF425594），与微小按蚊A和C同源。A、C两者ITS2序列22个固定位点存在碱基置换和插入／缺失，序列差异为4.8％；D3基因存在5个固定点突变，突变率1.5％。mtDNA-COII基因存在个体差异，但远小于同系其它成员如乌头按蚊、杰普尔按蚊间的变异。

Methods Twenty-three cases with rapid atrial fibrillation induced by severe aconitine intoxication were randomly divided into two groups, with 12 in treating group treated by ISHL alone and 11 in controlling group treated by conventional therapy, composed of such measures as gastric lavage and administration of emetics and cathartic.T...

对照组 1 1例给予催吐、洗胃、导泻、输液及抗心律失常治疗；治疗组 1 2例不经催吐、洗胃、导泻等排除毒物处理，给予双黄连注射液静滴。观察两组患者症状缓解时间及症状消失时间，并监测心电图恢复正常时间，比较两组结果的差异。结果治疗组患者症状缓解时间、消失时间及心电图恢复正常时间均明显短于对照组(P 。0 1 )。结论双黄连注射液对于乌头碱中毒所致快速性房颤有特别治疗效果

Observe the change cardiac rhythm and rate;The expression of Gi-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Methods: tachyarrhythmia was induced with aconitine, and acupuncture two-side "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事，那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来，随着部分预应力砼梁应用的推广，发现我国规范所采用的名义拉应力法的计算结果不稳定。