查询词典 zebrafish

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

Based on the study on cytogenetics of zebrafish, the genomes were compared between zebrafish and human, and the zebrafish Hox gene clusters were mapped to its chromosomes.

本文在斑马鱼的细胞遗传学研究的基础上，对斑马鱼与人类基因组进行了比较研究，同时对斑马鱼Hox基因簇进行了染色体定位研究。

The homology of the Myostatin sequences was analyzed among species. The results showed that the mature region DNA for Myostatin of Jinhua pig has the similarity of 100%% 97%, 95%, 95%, 93%, 93%, 93%, 89%, 90%, 89%, 87%, 87%, 86%, 86%, 86%, 85% and 76% compared with that of pig, horse, human, monkey, cow, hare, goat, sheep, house mouse, rat, goose, turkey, chicken, pigeon, Xiaoshan chicken, Fan duck and zebrafish respectively. The mature region DNA for Myostatin of Xiaoshan chicken has the identity of 99%, 97%, 93%, 92%, 92%, 87%, 86%, 86%, 86%, 86%, 85%, 85%, 85%, 84%, 84%, 83% and 74% compared with that of chicken, turkey, pigeon, goose. Fan duck, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively. The mature region DNA for Myostatin of Fan duck has the homology of 91%, 92%, 92%, 90%, 95%, 84%, 84%, 85%, 85%, 85%, 83%, 81%, 81%, 83%, 81%, 82% and 73% compared with that of chicken, Xiaoshan chicken, turkey, pigeon, goose, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively.

将测序结果与GenBank上其它物种的相应序列进行同源性分析，结果显示，金华猪Myostatin与哺乳动物猪、马、人、猴、牛、兔、山羊、绵羊、小家鼠和大鼠的同源性分别为100％、97％、95％、95％、93％、93％、93％、89％、90％和89％，与鸟类鹅、火鸡、鸡、鸽子、萧山鸡和番鸭的同源性分别为87％、87％、86％、86％、86％和85％，与鱼的同源性为76％；萧山鸡Myostatin与鸟类鸡、火鸡、鸽子、鹅和番鸭的同源性分别为99％、97％、93％、92％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为87％、86％、86％、86％、86％、85％、85％、85％、84％、84％和83％，与鱼的同源性为74％；番鸭Myostatin与鸟类鸡、火鸡、鸽子、鹅和萧山鸡的同源性分别为91％、92％、90％、95％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为84％、84％、85％、85％、85％、83％、81％、81％、83％、81％和82％，与鱼的同源性为73％。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Objective: We try to elucidate the probable mechanism of midblastula transition in zebrafish through study the cyclin C gene which is expressed maternally and zygotically during zebrafish embryo development.

斑马鱼胚胎发育过程中cyclinC基因母型和合子型表达的初步研究目的：通过研究cyclin C基因在母型和合子型的表达研究，阐释其在中囊胚过渡时期的可能作用。

This site provides image materials on fish development, including time-lapse recordings illustrate the morphogenesis of zebrafish embryos, from early cleavage to organ rudiment formation, fish epidermal cell movies, 3-D films of zebrafish gastrulation, and much more.

该网站提供有关鱼类发育的图像材料，包括阐明斑马鱼胚胎形态发生（从早期卵裂到器官初期形成）的时间间隔录象、鱼类表皮细胞影片、斑马鱼原肠胚形成的三维影片，等等。

Objective To identify biochemical genetic markers of outbred stock zebrafish AB, LF, and ST to develop a new genetic monitoring method for different zebrafish group.

目的 通过对AB、LF、ST（short tail，本地短尾群）三个封闭群斑马鱼的研究比较，建立封闭群斑马鱼的遗传生化标记。

NaF can induce significant increase of micronucleus frequency in eripheral erythrocytes of Zebrafish. Neither transgenic CryIA cotton seed powder nor regular cotton seed powder has obvious effect on micronucleus frequency in eripheral erythrocytes of Zebrafish.

腹腔注射氟化钠可以诱导斑马鱼外周血红细胞微核率的显著升高；转CryIA基因抗虫棉棉籽粉粉和普通棉棉籽粉粉一样，对斑马鱼外周血红细胞微核率无明显影响。

Zebrafish, androgenesis, female nucleus inactivation, UV, delayed fertilization

斑马鱼；雄核发育；雌核灭活；紫外线；延迟受精

The genetic inactivation of eggs is the first key stage of androgenesis in fish. In order to observe the effect of UV irradiation for the inactivation of female nucleus of zebrafish the eggs were irradiated by UV with solution-free, pure water, Ringer's solution and Cortland solution. The results showed that abnormal fry were obtained in all groups and haploids were validated. But irradiation with solution was confirmed it could not inactive fully female nucleus and the fertilization rate of the eggs irradiated with pure water was very low.

雌核灭活是雄核发育的第一个关键步骤，为研究紫外线对斑马鱼卵的雌核灭活效果，将斑马鱼的卵采用干法或置于纯水、Ringer氏溶液和Cortland鱼用生理盐水进行紫外线照射，结果表明，各种方法中均得到畸形鱼苗，并经染色体检查大多为单倍体；四种方法中在Cortland鱼用生理盐水中灭活效果最好，120 mJ/cm2紫外线可以使雌核完全灭活；干法处理不能达到雌核完全灭活的目的，在纯水中灭活后的卵子受精率较低。

There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式，可以避免教学形成模式後的僵化现象。