查询词典 virus

The four experimental virus strains and the three collate virus strains were divided into two groups, and belonged two evolutionary branches. Although ADV-DL1, DL2 and DL3 were all belonged to the Da Lian separated strains, they had the ulterior genetic relationship. The nuclear sequences of the obtained experimental ADV virus strains, ADV virus collate virus strains and typify virus strains of other four Parvovirus generic were cladogram analyzed with the Clustal W method of software DNA Star.

结果表明，ADV-Utah与四个实验毒株的序列同源性较高，同源率为92.9-93.4％，ADV-G、ADV-SL3与实验毒株的同源性较低；四个实验毒株和三个参考毒株被分别划归为两个组，分属于两个进化分支；实验毒株中ADV-DL1与DL2和DL3虽然同为大连分离株，却表现出较远的亲缘关系。

Results The highest positive rates were found in respiratory syncytial virus (RSV,30.5%) and adenovirus (AdV,27.4%),and the other findings were as following: Coxsackie virus (CV,11.3%), EB virus (EBV,6.5%),echovirus (ECHOV,4.8%),parainfluenza virus (PIV,1.6%), influenza virus B (IVB,1.6%) and influenza virus A (IVA,0%).

结果 呼吸道合胞病毒、腺病毒阳性率最高，分别为30.5%、27.4%，其次为柯萨奇病毒、EB病毒，分别为11.3%、6.5%，埃可病毒为4.8%，副流感病毒及流感病毒B均为1.6%，流感病毒A为0%。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Partial DNA-A sequence comparison with other geminiviruses showed that G7 were infected with 5 begomoviruses, these viruses were Papaya leaf curl China virus, Ageratum yellow vein china virus, Tomato leaf curl China virus, Euphorbia leaf curl virus and Tobacco leaf curl Yunnan virus.

得到部分G7的DNA一A序列，经NCBI检索和序列比较分析发现了5种菜豆金色叶病毒属病毒的部分DNA序列，分别是中国番木瓜曲叶病毒、中国胜红蓟黄脉病毒、中国番茄曲叶病毒、一品红曲叶病毒、云南烟草曲叶病毒。

Annual the tall hair that Spring Festival long holiday is virus period, during virus uses the Spring Festival, people each other sends a blessing, scanty to virus at the space that be on guard, send the link that contains virus or document through QQ or MSN, and once the user is clicked,the file is met toxic, toxic hind virus can send same instant message to all and online good friend, create instant news report user interlink infection.

每年春节长假都是病毒的高发期，病毒利用春节期间人们互发祝福，对病毒疏于防范的空隙，通过QQ或MSN发送带有病毒的链接或文件，而一旦用户点击此类链接或文件就会中毒，中毒后病毒会向所有在线好友发送同样的即时消息，造成即时通讯用户连环感染。

Biology technique is based on appearance symptom on sweet potato or Ipomoea Setosa after they are infected to identify virus. But it need long period and is apt to be limited by season. Based on coat protein of virus, serology detects virus during peculiar immunology reaction of antibody and antigen. But it can't detect virus without CP and preparing antibody need more time. The molecular biology method is based on nucleic acid to detect virus. This technique has high sensitivity, strong veracity, wide suitability etc.

生物学方法是依据病毒侵染后在甘薯或指示植物上的外观症状进行识别，但检测速度慢，易受季节限制；血清学技术是利用病毒外壳蛋白的抗原性，据特异性的抗体抗原免疫学反应检测病毒存在，但制备抗血清需时间长，对含量少和无蛋白外壳的病毒无法检测；分子生物学方法是以核酸为基础，通过检测病毒核酸来证实病毒的存在，该技术具有灵敏度高、特异性强、适应范围广等特点。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序，将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较，结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3％，与其它毒株的同源性在58.3％～91.7％之间；狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

The methods for determination of the complete genome sequences of potyviruses, carlaviruses and potexviruses were also established. The complete genome sequences of 13 viruses(20 isolates) were determined while 10 of them were the first report in the world.Five of them including Lily symptomless virus (NC_005138), Lily mottle virus(NC_005288),Onion yellow dwarf virus (NC_005029), Lily virus X (NC_ 007192) and Shallot yellow stripe virus(NC_007433) were selected as the standard sequences of those viruses by National centery of Biotechnology Information, USA.

建立了马铃薯Y病毒属、麝香石竹潜隐病毒属和马铃薯X病毒属成员的基因组全序列扩增技术，完成了13种不同植物病毒近20个分离物的基因组全序列测定，其中10个病毒的基因组全序列为国际首次报道，百合无症病毒(NC_005138)、百合斑驳病毒(NC_005288)、洋葱黄矮病毒(NC_005029)、百合X病毒(NC_ 007192)和葱黄条病毒(NC_007433)等5条序列被美国国家生物技术信息中心列为相关病毒标准序列的有。

The study was aided by the Ailuropoda Fund and designed on account of the Chengdu zoo' s topic that the Detection of the feline distemper' s serum antibody and the foundation of its immune programme about the grow wild feline animal such as Panthera pardus L, et al. The study regarded the attenuated feline panleukopenia virus as the diagnostic antigen which was isolated from the tri-combinant vaccine of Feline rhinotracheitis virus, calicivirus and panleukopenia virus . According to the tesults of Virus Physical and Chemical Character Detection, Agar Diffusion Reaction and Virion Shape Electron Microscope Observation, it was affirmed that the virion which was isolated from the tri-combinant vaccine was feline panleukopenia virus .

本研究从猫瘟热病毒、猫鼻气管炎病毒、猫鼻结膜炎病毒三联弱毒活疫苗中分离猫瘟热病毒弱毒株，通过对分离株进行病毒理化特性检测、双向琼脂扩散试验检测和病毒粒子形态电镜观察，最终确认从三联弱毒疫苗中分离到猫瘟热病毒，并以此分离株作为诊断抗原。

In this new architectural plan the Garden of Golgotha came to be at the centre of the new city.

Aelia 是高举他自己，并在此城内朝拜罗马诸神。

The continuous nature of peat accumulation means that terrestrial coaly rocks may provide a higher resolution and more complete record of base-level change than the time-equivalent shallow-marine strata, thus enabling the identification of highfrequency cycles that are not recorded in themarine strata.

泥炭积累的持续性质的手段，陆地coaly岩石可提供较高的分辨率和更完整的记录基准面变化的影响时，相当于浅海阶层，从而使确定的高频周期，不记录在themarine 阶层。