查询词典 vector analysis

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

First,wavelet transform decomposes the original image,then the coefficients of the three directions are used to construct the band-cross vector,and then the vectors are classified.In addition,new vector quantization technologies are used in this algorithm.

该算法在对图像进行多级小波变换后，构造三个方向的跨频带矢量，同时采用分类矢量量化，非线性插补矢量量化和基于人眼视觉特性的加权矢量量化，提高了图像的编码效率和重构质量。

Aimed at the problem that the traditional support vector machine cannot adapt itself to text database updating continuously from time to time, a text categorization algorithm with increment al SVM was presented after analyzing the KKT qualification of newly added text sets and thoroughly investigating the variation of support vector set due to text addition.

针对传统SVM无法适应文本数据库随着时间不断更新的问题，通过对新增文本集的KKT条件的分析，研究了加入新增文本集后支持向量集的变化，提出了使用增量SVM进行文本分类的算法，并通过实验验证了通过该算法得到的分类器和传统分类器有着相似的分类能力和泛化能力

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

Vector algebra in 3D, vector- valued functions, gradient, divergence and curl, Taylor series, numerical methods and applications.

三维向量代数、向量值函数、梯度、散度和旋度、泰勒级数、数值方法和应用。

A vector bundle including zero vector must be linearly dependent.

所以，当m为偶数时，此线性方程组有非零解，向量组线性相关

A new dynamic vector Preisach model that takesaccount of the anisotropic characteristics of media and vector reversible component ispresented.

第五章对旋转磁滞问题和交变磁滞问题进行了研究，分析了一些典型的交变磁滞模型，提出了一个新的考虑媒质各向异性及矢量可逆行为的动态矢量Preisach模型。

The paper based on component technology and COM, study and design the vector graph component, realize the function of vector graph, such as the input and output of map, the display of map, mutual plot with mouse, etc.

本文以组件技术和组件对象模型(COM，Component Object Model)为基础，研究和设计GIS矢量图形组件，实现GIS系统的矢量图形功能，如地图的输入输出、地图的显示、鼠标交互绘图等。

The studies on the developing of new media are very important to the increasing of the area density of HDD and to the implementation of hybrid recoding technology.

光磁混合记录方法，是一种可以突破超顺磁极限的限制，并进一步提高硬盘记录密度和读写速率的一种新型超高密度信息存储方式。

In io, mTOR inhibition promotes T cell anergy under conditions that would normally induce priming.

体内研究中，在正常可激发免疫反应的条件下，抑制mTOR可促使T细胞无能。