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2B8a was weakly reactive to neutrophils (23.72%) and negative for T cells, NK, DC, RBC and Plt. The antibody reacted to all 3 marrow CD34+ cells with an average positive rate of 39.33% while it was negative for G-CSF-mobilized CD34+ peripheral blood stem/progenitor cells (PBSC, 1.25%). Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34+细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34 细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

The results were as follows:The unit-roots characters (weight, length etc.) and 15N absorption decreased progressively downward. 15N absorbed by any unit roots could be transported from root to tiller and from tiller to tiller, but the transporting ratio was different.The pattern was as follows: when 15N was offered to total roots of plant or only to primary roots, it was transported to main stem and then decreassingly to tillers from lower unit to upper one, when 15N was offered to n unit-root, it was transported to n unit tillers, then to n + 2 or n-2 unit tillers , followed by n + 1 or n -1 unit tillers , and finally to other unfeed roots.

各单位根吸收(15)~N后，蘖根之间，蘖蘖之间皆可互相运转，但其运转比例不同，其功能模式是：(15)~N喂全株根或专喂初生根，则先供给主茎，其次是分蘖，而且随蘖位升高而递减；喂n单位根，则先供给n单位本身，其次是n+2或n-2单位的蘖，然后是n+1或n-1单位的蘖，最后是未喂(15)~N的根系。

Welded pipe unit: The stainless steel welded pipe unit, the cold bending formation series welded pipe unit, the high frequency welded pipe unit, the pot galvanize unit, cut the butt welding machine, the spiral welded pipe unit and the heavy-caliber straight seam bury the arc-welded pipe unit and so on the complete set of equipment and so on;..

焊管机组：不锈钢焊管机组、冷弯成型系列焊管机组、高频焊管机组、热镀锌机组、剪切对焊机、螺旋焊管机组及大口径直缝埋弧焊管机组等成套设备等；。。

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果：1、白藜芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长，同时促进细胞的分化和凋亡：白藜芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化，UW228-3、Med-3细胞向神经元细胞方向成熟分化，以100μM作用48小时效果显著；2、四个细胞系均有Fas和Caspase-3表达，但无Fas-L基因表达和蛋白活性产生，因此难以形成Fas相关性死亡通路；3、白藜芦醇处理前后CYP1A1基因的表达在各细胞系略有不同：UW228-1，-2，-3三个细胞系白藜芦醇以剂量相关性方式上调CYP1A1的表达，而Med-3细胞系，白藜芦醇却抑制CYP1A1的表达；四个细胞系在白藜芦醇处理后CYP1B1的表达均受到抑制；4、UW228-3细胞系经白藜芦醇作用后细胞周期有较大改变，表现在G0/G1期在整个细胞周期中所占的比例增加，而其它时期则相应减少。

The living cell of L428 cell has experienced from monoclonal to the multi-cell forming process,and presents a big cell around many small clone cell encystation phenomenon.finally,gigantic cells is dead in the life time.3.The cell counting result showed that the proportion of big cell or the H/RS type cell(diameter≥25um)is for(11.6±1.5)%in the L428 group,for(4.6±0.7)%in L428-MVC group and for(13.1±1.3)%in L428-EVC group,respectively.

经持续稀释成单个大细胞、单个小细胞的L428细胞，小细胞可分裂转化成大细胞，大细胞亦可生成小细胞；常见单个大细胞周围出现多个小细胞围绕的现象，在此过程中NF-kB一直持续活化。3。

Rapid cell selected, regional, rows or columns: the mouse click to select the current cell; click the region to be selected first cell, and then drag the mouse to the last cell can be two selected region between; worksheet click the Select All button, you can select the current worksheet; hold down the Ctrl click or drag using the mouse, you can select multiple nonadjacent cells or regional; select a region first cell, hold down the Shift key click on the angle of regional cells, can be between the selected rectangular area; click the line number can be selected corresponding to the entire line; click out the corresponding label can be selected out of the whole; along its line number or a list of drag the mouse, or to select the first row or first column, hold down the Shift key to select the end of the row or column, you can select a number of adjacent rows or columns; first select the first row or first column, and then hold down the Ctrl key to select other non-adjacent rows or columns; If you want to increase or decrease in the activities of the cell area can be selected by holding down the Shift key click the lower right corner of the region the last cell, the cell activities and the click between the cells will become a rectangular area to select a new region.

快速选中单元格、区域、行或列：鼠标单击可以将当前单元格选中；单击待选中区域的第一个单元格，然后拖动鼠标至最后一个单元格，可以将两者之间的区域选中；单击工作表的全选按钮，可以将当前工作表选中；按住 Ctrl 单击或使用鼠标拖动，可以选中不相邻的多个单元格或区域；选中某区域的第一个单元格，按住 Shift 键单击区域对角的单元格，可以将两者之间的矩形区域选中；单击行号可将对应的整行选中；单击列标号可将对应的整列选中；沿行号或列表号拖动鼠标，或者先选中第一行或第一列，按住 Shift 键选中结束行或列，就可以选中相邻的多个行或列；先选中第一行或第一列，然后按住 Ctrl 键选中其他不相邻的行或列；如果您想增加或减少活动区域中的单元格，可以按住 Shift 键单击选中区域右下角的最后一个单元格，活动单元格和所单击的单元格之间的矩形区域就会成为新的选中区域。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

In order to research the relations of carbon sources to structure of PHAs, six kinds of PHAs sample were synthesized by activated sludge with six different carbon sources respectively in the aerobic-anaerobic-anoxic process. Their structures were determined by 〓HNMR、〓CNMR and GC-MS. When the carbon source was acetate, the unit composition of PHAs was 93. 91mol％ 3-hydroxybutanoic acid and 6. 09mol％ 3-hydroxypentanoic acid ; When the carbon source was propionate, the unit composition of PHAs was 28. 66mol％ HB, 63. 13mol％HV, 2. 55mol％ 2-methy-3-hydroxy-butanoic acid (2MHB) and 5. 66mol％ 2-methyl-3-hydroxypentanoic acid (2MHV); When the carbon source was 80％ butyrate and 20％ 1, 4-butanediol, the unit composition of PHAs was 65. 03mol％ HB, 28. 06mol％HV, 1. 91mol％2MHB, 2. 69mol％ 2MHV, 0.73mol％ 4-hydroxy butanoic acid (4HB), 0.78mol％ 4-methyl-3-hydroxy-pentanoic acid (4MHV), 0.80mol％ 3-hydroxyhexanoic acid ; When the carbon source was 20％ butyrate and 80％ 1, 4-butanediol, the unit composition of PHAs was 61. 39mol％ HB, 23. 01mol％HV, 4. 58mol％2MHB, 5. 97mol％ 2MHV, 0.91mol％ 4HB, 2. 37mol％ 4MHV, 1. 77mol％ HHx; When the carbon source was 60％ pentanoate and 40％glucose, the unit composition of PHAs was 41. 24mol％ HB, 58. 76mol％HV; When the carbon source was 60％ benzoate and 40％ glucose, the unit composition of PHAs was 65. 48mol％ HB and 34. 52mol％ HV.

为了研究不同碳源与产物PHAs结构的关系，采用好氧-厌氧-缺氧模式利用6种不同碳源培养活性污泥得到6种不同的PHAs样品，通过〓HNMR、〓CNMR和GC-MS谱图确定了这6种PHAs的单体成分和比例：以乙酸钠培养活性污泥得到的PHAs的单体组成是93.91mol％3-羟基丁酸和6.09mol％3-羟基戊酸；以丙酸钠培养活性污泥得到的PHAs的单体组成除28.66mol％HB、63.13mol％HV外，另外还有2.55mol％3-羟基2-甲基丁酸（2MHB）和5.66mol％3-羟基2-甲基戊酸（2MHV）；以80％丁酸钠和20％1，4-丁二醇混合溶液培养活性污泥得到的PHAs含有七种单体组成：65.03mol％HB，28.06mol％HV，1.91mol％2MHB，2.69mol％2MHV，0.73mol％4-HB（4-羟基丁酸），0.78mol％4MHV（3-羟基-4-甲基戊酸），0.80％HHx（3-羟基己酸）；以20％丁酸钠和80％1，4-丁二醇培养活性污泥所得到的PHAs含有与3#样品相同的七种单体，只是比例不同，分别是61.39mol％，23.01mol％，4.58mol％，5.97mol％，0.91mol％，2.37mol％，1.77mol％；以60％戊酸钠和40％葡萄糖培养活性污泥所得到的PHAs由HB和HV组成，比例是41.24 mol％∶58.76 mol％；以60％苯甲酸钠和40％葡萄糖培养活性污泥所得到的PHAs也由HB和HV组成，比例是65.48 mol％∶34.52 mol％。

The invention provides a system used for recycling urban automobile-washing water, which is a system formed by organic combination of a pretreatment unit, a mechanical treatment unit and a control unit, wherein, the pretreatment unit comprises a fence and a sedimentation tank, and chemical treatment; the fence is arranged on an inlet of the sedimentation tank; the mechanical treatment unit adopts a centrifuge and is arranged on an outlet of the sedimentation tank; and the control unit comprises a peripheral circuit, a singlechip and a variable-frequency motor.

本发明提供的城市洗车用水循环利用系统，是由预处理、机械处理和控制单元的有机组合来的系统，其中：预处理单元包括栅栏以及沉淀池和化学处理，栅栏设置在沉淀池的入口处；机械处理单元采用离心机，它设置在沉淀池的出口处；控制单元包括外围电路、单片机和变频电机。

Molecular Distillation is a kind of high vacuum distillation method, which is suitable for the separation of high boiling, heat sensitivity and viscidity products.

分子蒸馏技术是一种在高真空条件下进行的连续蒸馏过程，适合于分离高沸点、热敏性及具有生物活性的混合物。

They are to the Noughties what yuppies were to the Eighties .

那在六十年代是时髦的，但在八十年代肯定是过时了