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By introducing the NCP function, the KKT conditions of POPF system are transformed equivalently. Based on the transformed nonsmooth nonlinear algebraic equations, the FOSMM is used to determine the POPF model expressed by the numerical characteristic of variables. The model includes nonsmooth functions, so it can be solved by a semismooth L-M method based on the subdifferential.

将概率最优潮流表述为随机非线性规划问题后,利用非线性互补函数将该问题的KKT条件进行转化,基于转化后的非线性代数方程组,利用一次二阶矩法确定了以待求量的数字特征表示的概率最优潮流模型,由于该模型包含不光滑函数,因此采用基于次微分的半光滑L-M方法求解。

In the POPF model, wind farm is modeled by the probabilistic wind farm model considering the reactive power-slip characteristic, and the inequality constraints include not only the unit output constraints, the ratio constraints, the voltage constraints and the line current constraints but also the reactive compensation capacity constraints in wind farm and the system climbing capacity constraints per minute. By introducing the NCP function, the KKT conditions of POPF system are transformed equivalently. Based on the transformed nonsmooth nonlinear algebraic equations, the FOSMM is used to determine the POPF model expressed by the numerical characteristic of variables. The model includes nonsmooth functions, so it can be solved by a semismooth Newton-type method based on the subdifferential.

概率最优潮流模型中,风电场采用考虑无功功率—滑加热器差特性的风电概率模型,不等式约束中除了机组出力约束、有载调压变压器变比约束、电压约束和支路电流约束,也考虑了风电场无功补偿容量约束、系统的分钟级爬坡能力约束;使用非线性互补函数将概率最优潮流的KKT条件转化为一组包含有不光滑函数的非线性代数方程组,然后基于一次二阶矩法确定了以待求量的数字特征表示的POPF模型,由于该模型包含不光滑函数,因此采用基于次微分的半光滑牛顿型方加热器法求解。

At the same time, intact shuttle-plasmid pZ189 was also introduced into the host cell which was used for detection of nontargeted mutation frequency. After replicating in cells, progeny plasmid DNA was isolated and transformed into E coli. MBM7070.In the presence of ampicillin, X-gal and IPTG, pZ189-transformed E. Coli MBM7070 forms blue colony if the plasmid contains an active SupF suppresser tRNA gene, and white or light blue colony if the SupF tRNA gene is inactive.

但是由于检测非定标性突变需同时引入穿梭质粒pZ189在细胞中复制,并回收质粒DNA,通过转化宿主菌E.coliMBM7070,在含x-gal和IPTG的氨苄指示板上培养,如果用于检测突变的靶基因SupF tRNA基因发生突变,则菌落色泽为白色或淡蓝色,而正常者为兰色。

For inheriting the dynamic constraint information of the multibody simulation model, topological configuration of the multibody simulation model was built, and simplified constraint form was put forward with the topological mapping of the multibody simulation model in the heterogeneous systems. To transform the information of the multibody simulation model, the offset matrix which transformed the location and orientation of constraints and the matrix which transformed the location and orientation of parts were computed.

利用多体动力学仿真模型在异构系统的拓扑映射,建立多体动力学仿真拓扑构型,提出仿真模型动力学约束的约束简型表示法,实现动力学约束信息的关联继承,并通过约束偏置变换矩阵和构件位姿变换矩阵的求取,驱动多体动力学仿真模型构件和约束位姿的自动调整,实现多体动力学仿真模型的动态转化。

From the tests,we have obtained some structural variant features related with salt resistance and demonstrated the successful introduction of Rhizophora apicularta DNA into tomato.This thesis contains two parts. In the first part,the morphological and anatomical structures of the leaves were investigated. As to the morphology,some difference between control and transformed tomato were found. The leaf dimensions of the transformed tomato with unextended leaves were smaller than that of control. These structure changes should help to decrease the evaporation of water effectively and release the physiological drought resulted from saline.

本实验分两部分:第一部分、对转化番茄的形态及细胞显微结构进行观察与分析,实验表明转化番茄形态与对照相比有明显的变化,其叶面积变小,叶形收拢,这种结构可有效地减少水分的蒸发,缓解由盐胁产生的生理干旱;组织解剖结构结果显示,耐盐番茄在细胞结构上发生了明显的变异:其气孔器倾向下陷,表皮毛增多,叶脉微管组织不发达,微管束周围出现增多的贮水细胞,叶缘及其它部位表面分化出大型的多细胞分泌型结构,这些结构与转化番茄后代较强的耐盐能力有关。

The Klason lignin level of anti-4CL-transformed tobacco was much lower than that of the control. The cellulose content increased in transgenic tobacco with UGPA constructs.We also obtain transgenic tobacco transformed with Acetobacter xylinum UGPase gene.

研究了木醋酸菌来源的UGPase基因与植物来源的UGPA基因在转基因烟草中的表达,测定了两种转基因烟草的尿苷二磷酸葡萄糖焦磷酸化酶的活性、木质素含量和纤维素含量。

Compared the lignin and cellulose level of these two kinds of transgenic tobacco, we found that the cellulose content increased and the lignin content deduced in transgenic tobacco transformed with Amorpha fruticosa UGPA constructs, both the cellulose content and the lignin content increased in transgenic tobacco transformed with Acetobacter xylinum UGPA constructs.

结果表明,两种转基因烟草的尿苷二磷酸葡萄糖焦磷酸化酶的活性均有明显提高;转紫穗槐UGPA基因的烟草纤维素含量有所提高,木质素含量略有降低,纤维素与木质素含量之大连理工大学博士学位论文比提高;而转木醋酸菌来源的UGPase基因的烟草木质素含量和纤维素含量均显著提高。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中,得到的重组质粒命名为pGBKT7-Gα,β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性,将其转化到酵母菌pJ69-4A中,再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达,且在未成熟卵母细胞中,MⅠ期的表达明显强于GV期的表达;②RT-PCR产物克隆测序结果显示:昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp~1153bp)]完全一致;Blastn比对结果发现:在不同物种中差异较小,选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析,提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%,100%,100%,36%,100%,100%,67%,100%,100%,92%,99%,99%,99%;Clustal 1.81和Treeview构建出的分子进化树表明:在上述14种生物中昆明小鼠与中国仓鼠进化关系最近,与兔子、猪聚成一簇,与食蟹猴进化关系最远;③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段,重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The four articles show the preliminarily philosophical transmutation on the view of moral between heaven and human made by Confucius on the basis of the ideas of heaven and human formed from the Xia, Shang,Western Zhou dynasties to the Spring and Autumn period; they reveal Confucius' orientation to think which transformed the traditional heaven as dominator into the moral heaven, and transformed the relationship between the subject and the metaphysical existence from external transcendence to innerness; thus they relieve Confucius as a keen and profound thinker at that time.

本文通过对帛书易传《二三子问》、《易之义》、《要》、《缪和、昭力》四篇的分析,认为帛传四篇为孔子《易》论,其中包涵着《论语》、《孔子集语》、传世本《易传》等反映孔子思想的传世文献所隐微罕见的天人道德观念;帛传四篇表现了孔子在涵括三代以至春秋新旧天人观念的基础上对于天人内涵的初步的哲学改造,反映了孔子将传统的主宰之天转化为道德之天、并将传统的主体对于形上存在的外在超越关系转化为内在超越关系的致思取向,从而凸显了孔子作为当时敏锐而深邃的思想家的形象;帛传四篇所涵具的孔子天人道德思想补足了三代天命德政观与思孟天道性命思想之间的逻辑缺环,是经由曾子而达致《中庸》、郭店儒家简、《孟子》乃至传世本《易传》所体现的天道性命思想高峰的理论根据。

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