查询词典 tittering

He sponged out lines and remade them; but he only distorted them more than ever, and the tittering was more pronounced. He threw his entire attention upon his work, now, as if determined not to be put down by the mirth.

要不是她也和别的仙女一样轻抚人间，令人神奇为之震颤，她会像浮云一般让人不知不觉，消失得无影无踪。

Edith, Ethel, Gerty, Lily. Their likes: their breaths, too, sweetened with tea and jam, their bracelets tittering in the struggle.

跟他们类似的人，她们的呼吸也给红茶、果酱弄得甜丝丝的，扭动时，她们腕上的镯子在窃笑着。

The congregation being fully assembled, now, the bell rang once more, to warn laggards and stragglers, and then a solemn hush fell upon the church which was only broken by the tittering and whispering of the choir in the gallery.

教堂里一片寂静，显得十分庄严，只有边座席上唱诗班里有些低声嘻笑和说话的声音，打破了这种寂静，而且自始至终整个布道过程，唱诗班里一直有人在窃窃私语，低声说笑。

A cDNA library with a tittering of 7+106 pfu/mL was constructed basing on the cotton boll shell (10 days post anthesis, dpa) RNA obtained above. The recombinant ratio was found to be ca. 90% by blue-white counting.

首次构建了构建了花后10天棉铃壳cDNA文库，未稀释文库滴度达到7×10~6，蓝白斑计数计算重组率为90％，为棉铃发育基因及其启动子的克隆研究打下了基础，目前国内外未见类似报道。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

More haunting still was the incessant tittering of the desert bats as they swooped low over the oasis pond to scoop up tiny mouthfuls of water.

嗯嗯，果然刚过四级就来翻译还是很有困难的说

Fro m somewhere below comes faint, ghastly tittering.

某处传来模糊的，恐怖的吃吃的笑声，在囚楼中漂动。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Who? I never heard of him, Paul said, before asking teammate James Posey if he had heard of him.

赛后，科比说，他一直都是一名非常出色的射手，今天他打得很棒。

When I joined the company, I rotated around the different sections.

我加入这个公司时，轮换过几个不同的部门。