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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

Technology relatively complicated many throw projects javelin throw, its intact technologies through holding a javelin the run-up win most heavy speed, Join and throw step and get momentums, act on the end of the breaking out type the axis of ordinates of the javelin hard, Make javelin at suitable angle, throw and produce at shoulder javelin.

掷标枪技术是比较复杂的多轴旋转的投掷项目,其完整技术是通过持枪助跑获得最大的速度,连接投掷步获得动量,通过爆发式的最后用力作用在标枪的纵轴上,使标枪能沿着适宜的角度,将标枪经肩上掷出。

Currently, in the oil-field exploitation, existing the phenomenon of garbling the lack thickness of stratum with vertical fault-throw in well logging identification of strata while meeting fault in drilling well. In most case, consider the lack thickness of stratum which contrast the imperfection geologic strata thickness to the well and the well as the fault-throw. The method which using very popular in oil-field exist shortages, it very much easily lead people into the areas of misplay. especially when the seismic person and geology confirm the fault-throw, It often appear inconsistent instance.

目前,在油田开发中钻井钻遇断层时,地质人员时常存在将测井分层与相邻井对比时缺失地层厚度与铅直地层断距混淆的现象,很多情况下把井与井对比缺失地层厚度等同于断层断距等,这些现今在油田非常流行的用法存在一些不足之处,容易把人们引入一些认识上的误区,特别是在地震与地质人员进行断层断距的确定时,经常出现矛盾的情况。

Gary: I will be competing in all the throwing events: the discus throw, the hammer throw, the javelin throw, and the shot put.

盖瑞: 我要比所有的投掷项目,包括铁饼、链球、标枪和铅球。

8 A foul by either team:* Before the beginning of a period other than the first period, or * During the alternating possession throw-in, Does not cause the throw-in team to lose that alternating possession throw-in.

比如跳球后是B队拿到了球,第一节没有交替发生,那第二节本该A队发球。虽然A队在之间发生技术犯规,但是这个犯规不能使A队失去第二节开始的球权,仍然应该是B队2罚后由A队开球。

When the fault throw is relatively small, the shale gouge ratio and clay smear potential are relatively large and shale smear is strong. In addition, the coal layers on both sides of the fault are mainly juxtaposed against shale. Therefore, shale smear and juxtaposition play a key role in the sealing. In contrast, when the fault throw is relatively large and shale smear is relatively weak, juxtaposition is unfavorable for sealing. However, the mylonite resulted from strong cataclasis can act as a seal of the coals. The cementation resulted from underground water activity is of great significance to the sealing ability of the fault. Mylonitic coal layers resulted from faulting occur on both sides of the fault. They are unique reservoirs with low permeability and high displacement pressure and their scales depend, to some extent, on the fault throw, fault properties, the hanging wall and footwall.

结果表明:①当断层落差较小时,断层泥比率和泥岩涂抹潜势值较大,泥岩涂抹效应强烈,且与断层两侧煤层对接的岩石主要为泥岩类,此时起封闭作用的主要为泥岩涂抹作用和断层两侧岩性配置关系;②当断层落差较大,泥岩涂抹作用较弱时,对接越不利于封闭形成,但碎裂作用强烈,沿断层两侧形成的糜棱岩对煤层起到了封闭作用;③地下水活动导致的胶结作用对整个断层的封闭具有重要的意义;④断裂作用形成的糜棱煤沿断层两侧分布,此糜棱煤带是一种低渗透、高排替压力的特殊储层,其规模与断层的落差、性质、上下盘都有一定关系。

Set the fashion set fire to set a trap substitute for sth. take into account take account of take advantage of take care take care of take a chance take charge of take comfort in take command of take into consideration take delight in take one's departure take effect take one's farewell of sb. take / give a glance at throw / cast light on/upon take notice of take /make an objection to take pains take part in take place take the place of take possession of take pride in take the resolution to do sth. take the responsibility for take / bear one's share of take the firm stand take a stand for sth. take one's temperature take one's time take turns thanks to throw a scare into sb.

在------中设置障碍对-----提出要求给某人树立榜样创立新式样点燃设陷阱代替物考虑考虑利用,趁-----之机注意,当心照料,照顾冒险,投机接管,管理在------中得到安慰指挥考虑以------为乐动身,出发生效,起作用向某人道别对-----初略地看一下使人了解某事注意到反对----努力,尽力参加发生,进行取代,代替占有,占领以-----自豪决心做某事负起做某事的责任承担自己的一份坚定立场赞成某事量体温不着急依次,轮流由于吓坏某人使逊色,使相形见绌碰运气经不起诱惑知识改变命运,思路决定人生!

Throw your trash into the trashcan or when you see some trash throw it into the trashcan.

当你去公园的时候,请保持干净。

Main control circuit adopts electric controlled to exchange electronic switch technology and passing zero detection techniqueing advanced electronic device, which realize to pass zero level and smooth to its cutting ,shuting floodgate and bludgoning into , no over voltage when operating it , there is no electric arc to fire again to shove to have to throw condenser bank, condenser need is it can come into operation again to discharge, its cutting restriction of number of times to throw.

主控制电路采用电力电子器件可控交流电子开关技术和先进的过零检测技术,实现电容器组的过零平滑投切、无合闸涌流冲击、无操作过电压、无电弧重燃,电容器无需放电即可重新投入使用,并且不受投切次数的限制。

Field events are further divided into jump and throw; jump includes high jump, pole jump, long jump and triple jump; throw includes shot, discus, javelin and hammer.

田赛又分成两类,分别是跳和掷,跳包括跳高、撑竿跳高、跳远及三级跳远;掷则包括铅球、铁饼、标枪及链球。

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推荐网络例句

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作,并且很受欢迎,"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型,我们必须经过如图2所示的简化步骤。

Rev. 20:15 And if anyone was not found written in the book of life, he was cast into the lake of fire.

启二十15 无论谁在生命册上不见是记著的,就被扔在火湖里。