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The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明，制得的兔抗PRRSV IgG纯度较高，具有良好的特异性，可用于原位检测病猪肺组织中PRRSV抗原的分布；Envision法的检测结果显示，PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内，其次为肺泡上皮细胞和细支气管黏膜上皮细胞；通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测，均有较高的阳性细胞表达，平均阳性细胞率为46.5%。

Males should also be tested for brucellosis.

公犬也同样应当检测布鲁氏菌病。

Methods: Percutaneous adsorption of brucine solution were tested by improved Franz diffusing cells in vitro.

用改良的Franz扩散池法对不同浓度的马钱子碱溶液进行体外透皮实验的考察。

Integrative analysis for the physiological factors and anatomical structure shows that, among the 12 provenances of Betula alnoides Buch Ham tested, K、D、G、B are those that are more cold-resistant, while F、A、C、K are the ones that are more hot-resistant.

对西南桦种源生理指标与解剖结构进行综合分析发现，所试12个种源中较抗寒为种源K、D、G、B；较抗热性的种源为F、A、、C、K。

With Lanzhou lily as tested material, the influences of development degree of lily bud scale, scale segmentation mode and sterilizing medicament on the cutting propagation rate of lily bud scale were studied.

方法］以兰州百合为试验材料，研究百合鳞片的发育程度、鳞片分割方式和消毒药剂对百合鳞片扦插繁殖率的影响。

This experiment was carried out during 2002~2004 in Shan-dong Agricultural University. The dynamic changes of respiratory metabolism in dormant bud of two nectarine cultivars, Wuyuehuo and Shuguang, which have different Chilling requirements, were studied in the experiment. The soluble sugar, starch, soluble protein and amino acid in flower bud and scale, temperature, regulator, and shading were tested to discuss their effects on the respiratory metabolism.

本试验于2002年10月至2003年12月在山东农业大学进行，以需冷量不同的油桃品种曙光、五月火为试材，测定了不同需冷量品种的油桃花芽冬季休眠期间呼吸代谢的动态变化，以及呼吸基质及蛋白质和氨基酸的动态变化，探讨了呼吸代谢与自然休眠的关系；同时又测定了温度、遮荫、除芽鳞片以及生长调节剂对呼吸代谢的影响效果，探讨了影响油桃休眠的外界因子。

With seeds of buffalo grass Zhongping 1 as tested materials, the proteins were extracted from seeds by TCA/A precipitation and Trizol method and analyzed and compared by bidirectional electrophoresis system with uniform conditions of protein.

方法］以"中坪一号"野牛草种子为试材，分别采用TCA/A沉淀法和Trizol法提取种子蛋白，并利用条件一致的蛋白质双向电泳体系进行分析比较。

Using the tissue cultured bulblet of narcissus cultivar Fortissimo as tested materials, with 1/2MS as the basic medium, 4 conch, of NAA and IBA including 1.0, 0.5, 0.1 and 0.05 mg/L were set up to study the effects of hormone on rooting induction of tissue culture seedlings in narcissus.

方法］以水仙栽培品种Fortissimo的组织培养小鳞茎为试验材料，以1/2MS为基本培养基，设计1.0、0.5、0.1，0.05mg/L 4个NAA、IBA浓度，研究激素对水仙组培苗生根诱导的影响。

In nearly 50 years in business, the company has tested over 10,000 bulk solids.

在近50年的业务中，该公司测试了10000多个固体材料。

Even the long-tested Burberry claimed earlier this year that they had decided to move the produce lines into China Mainland.

就连久经考验英国国宝级的Burberry也在今年的早些时候宣布了，要把生产线搬到中国的广州。

This paper designs the communication system of radio telescope FAST.

针对500m口径球面射电望远镜FAST分布式控制系统的特点完成了其通信系统的设计。

Political sheep's step-by-step a large number of fossils unearthed in the county soil Ma Ali after rural village in the middle of sallow pelitic siltstone.

步氏和政羊化石大量出土于广河县阿力麻土乡后山村，产于灰黄色泥质粉砂岩中。