查询词典 terminating continued fraction

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

The stress-strain curve of new-old concrete and HPRRC, are suggested to be modeled by two sections: the ascending section of the stress-strain curve is simulated by cubic parabola equation and the descending section is simulated by rational fraction, where the theoretical value is in good agreement with the test value.

新—老砼及HPRRC应力—应变全曲线建议采用两段式拟合：上升段采用三次抛物线方程模拟，下降段采用有理分式模拟，理论值与试验结果吻合较好。

The Chinese herb that could dispel cold, promote blood circulation by removing blood stasis, and relieve pain, was powder composed of common monkshood root, arisaematis, pinelliae tuber, mastix, myrrh, pubescent angelica root, curare, and carthamus tinctorius, which was mixed with 0.75 volume fraction alcohol to make into a flat shape of 15 cm with gauze.

中药以散寒活血祛瘀止痛为主，将川乌头、天南星、生半夏、乳香、没药、独活、马钱子、红花等混合研成粉状，加入体积分数为0.75的乙醇搅拌成糊状，用沙布制成直径约15 cm饼状态物，敷于患处，6~12 d为1个疗程。

Source phase current is a fraction of the line current, which should come as no surprise.

来源期流通的是输送流水线上电流的一个部份，应该没有什么好奇怪的。

Tests of optimizing of size fraction match of bridging material and plugging fracture were curried out.

在分析长岭地区深层储层裂缝发育规律的基础上，提出无渗透桥堵技术。

The realistic face pressure is expected to be equal to earth pressure at-rest both for making face stability and decreasing fraction resistance between cutterhead of shield and soils during tunneling process.

考虑到减少刀具磨损和节约能源问题，推荐理想的掘进工作面土压力为静止侧向土压力。

The symcenter of conoscopic interference pattern is corresponding to vertical incident light and crystal birefractive index is proportional to interference order m. Decimal fraction of interference order m is calculated by the placement of central dark fringe and integer part is ascertained by self-consistent method.

干涉图的对称中心对应于正入射光线，双折射率正比于此点的干涉级m，m的小数部分主要由中央暗条纹的位置决定，m的整数部分由自洽的办法求出。

The CC solution with 7% mass fraction was exposed to solution spinning in presence of 7% dilute sulfuric acid as coagulation bath after filtration and deaeration.

探索了尿素对纤维素氨基甲酸酯/氢氧化钠溶液的增溶作用，考察了不同浓度CC溶液的流变性能，选择CC质量分数为7%的溶液经过滤、脱泡后，以7%的稀硫酸为凝固浴进行溶液纺丝。

The results showed that, under the same testing condition, the mixed solution, in which the mass fraction of amine is 40%, the volume ratio of DEA and MDEA is 1∶6, has the best desulphurization and decarbonization property.

试验结果显示：在相同的试验条件下，溶液中总胺为40%，DEA与MDEA体积比为1∶6配比制成的混合溶液其H2S和CO2负荷最高，溶液的脱硫脱碳性能最好。

During the construction of the model,the depth of surface enrichment layer,the 137Cs depth distribution pattern in enrichment layer,the 137Cs decay constant,the 137Cs input fraction,the dept...

在建立模型的过程中，充分考虑了137Cs的衰变常数，年沉降分量，耕层厚度和采样年份等因素。

Smoking cigarettes adds to the Computer Desk Computer manufacturer computer mouse Computer Computerized collar knitter machine Conduit Bodies construction board Container Bag Control Arm Cooking Thermometer general levels of air pollution as well as damaging our health.

吸烟不仅对健康有害，而且增加空气污染程度。香烟雾含有多种有害物质：不仅有致癌化学物质、有某种能阻止氧气进入血液的气体，还有引起血压增高、心速加快的被称为尼古丁的物质。

He is one of my favorite actors.

他是我最喜欢的男演员之一。