查询词典 template

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

This approach uses the geometric features and radiation features of the road, according to the small amount seed points given by the user, each point in the road centerline can be gotten. Then an adaptive gray template is used to adjust each point in the road centerline. Finally, least square method and Newton iteration method are adapted to each point in the road centerline to fit a polygonal line.

该方法利用道路地物的几何特征和辐射特征，根据用户提供的少量道路种子点，利用自适应灰度模板对道路中心线上的每个点进行调整，最后采用最小二乘算法和牛顿迭代法对调整后的道路中心线上的每个点进行拟合，以此获得趋近于道路中心线的最佳折线。

The data are divided into three parts:beat template,residual and position parameter.

所提出的方法，也可应用到图像数据和其它数据的压缩中

Your own token type do not need to take this Position template parameter, but please note, that the token type in any case needs to have an embedded type definition position_type .

你自己的单词类型不一定要带有这个 Position 模板参数，不过请注意，任何情况下，单词类型都需要具有内嵌的类型定义 position_type。

In this study, Chitosan-Poly complex nanoparticles were prepared by template polymerization of acrylic acid in the chitosan solution. The preparation and characterization of CS-PAA nanoparticles including the yields of the nanoparticles, FT-IR spectrum analysis, dynamic light scattering, zeta potential, and morphology of nanoparticles were concentrated in this study. The effects of temperature, weight ratio of CS and PAA, and pH value on the physicochemical properties of nanoparticles were studied.

本研究中，我们以模板聚合法在几丁聚醣（Chitosan， CS）溶液中聚合丙烯酸（Acrylicacid， AA）以制备几丁聚醣－聚丙烯酸复合奈米颗粒并计算其产率，再以傅利叶红外线光谱仪、动态光散射粒径分析仪（Dynamic light scattering， DLS）、表面电位测定仪及穿透式电子显微镜等仪器分析CS－PAA 复合奈米颗粒之性质，以探讨聚合时温度、聚合反应时间、CS 与AA 之重量比，以及介质pH 值对CS－PAA 复合奈米颗粒结构的影响。

Preprocess the digital images and get the samples in order to the template.

预处理数字图像，获得学习样本为得到模板做准备。

Download the spokesperson presentation with synchronized graphics template to learn how to put a video of a presenter on the web and synchronize graphics with key parts of the presentation.

下载发言人介绍同步图形模板，以了解如何把视频的主持人，并在网络上同步图形核心部分的介绍。

Therefore, the quality of the template, quality, and product in the market can completely guarantee prevalence.

所以对模板的品质、质量，以及产品在市场上的流行程度可以完全保证。

There had been a moment during the breakfast meeting, though, after the backslapping and the small talk and when all of us were seated, with Vice President Cheney eating his eggs Benedict impassively and Karl Rove at the far end of the table discreetly checking his BlackBerry, that I witnessed a different side of the man.

那次早餐会期间，在表示过关心和寒暄以后，我们所有人坐了下来，我注意观察了一下，副总统切尼面无表情地吃着班尼迪克蛋（Eggs benedict，源自美国，以英式松饼、火腿、水煮蛋以及荷兰酱组合而成），卡尔。罗夫在餐桌的远端谨慎地经常查看他的黑莓手机，我目睹了他所不为人知的一面。

Talk Undelete failed; someone else may have undeleted the page first.

Talk 无法删除选定的页面或图像（它可能已经被其他人删除了）。