查询词典 technique

Methods Density gradient centrifugalization combined with adherence method were used to segregate and cultivate rat MSCs. MSCs cultivated to the 3 rd passage were characterized using flow cytometry technique. TGF-β1 with 1, 2 and 5 μg/L were pretreated on MSCs for 8 h, then serum and oxygen deprivation were used to cause analogous ischemia of MSCs in vitro. AnnexinV/PI flow cytometry technique was used to evaluate apoptosis and survival of MSCs.

采用密度梯度离心结合贴壁法分离、培养大鼠MSCs，对培养第3代的MSCs进行鉴定；用1、2、5μg/L的TGF-β1分别预处理细胞8 h，然后用血清剥夺和低氧处理建立MSCs体外模拟缺血性损伤模型，通过流式细胞术评价MSCs的凋亡及存活情况；用Western blot方法检测2μg/L的TGF-β1对Akt和p-Akt表达的影响。

The genechip technique for Ceratitis fruit flies provided rapid detection and species identification method to quarantine fruit flies from imported fresh fruits and vegetables in China. Meanwhile, the technique also could be used in detecting other tephritid and related pests in plant quarantine.

地中海实蝇生物芯片检测技术将为我国进口果蔬中检疫性实蝇快速筛查和种类鉴定提供检测方法，同时，还可应用到其他属的实蝇以及相关害虫的检疫中，为有害生物的快速鉴定提供了新方法。

The Passive Optical Network based on Asynchronous Transfer Mode combines the ATM technique with the PON technique, so it can support different services and has the capability to transfer information from service network interface to user network interface changelessly.

基于异步传输模式的无源光网络结合了ATM的多业务多比特率支持能力和PON的透明宽带传送能力，其光纤入户模式是宽带接入技术的发展方向。

For the purpose of optimizing the technological parameter for chimoncanthus tea scenting technique, the influence on quality of chimonanthus tea for the single scenting technique factors by means of Two Regression Orthogonal Combination Test was investigated.

采用二次回归正交组合试验方法，研究一窨一提窨制技术因子对蜡梅花茶品质的影响，优化蜡梅花茶窨制的最佳工艺参数。

And the calculator and the network technique is implicating in all fields of the society. University is an important base of train academician; finance office is an important function department. It's an arduous task for finance officers of university that to integrate information technique and finance offices nicely, expedite the schedule of information programming, and introducing information network and advance software to finance offices, it is also clamant.

高校，是培养人才的重要基地，财务部门是高校的重要职能部门，如何把信息技术和财务管理很好地结合起来，把信息网络和先进的计算机软件技术引入财务工作，加快只能部门的信息化进程，是摆在每个财务工作者面前的重要问题，同时也是一个迫在眉睫的问题。

The comparison group uses the pure non-pain induced abortion technique,the experimental group uses the visible non-pain induced abortion technique,observes and records two groups of patients' the formation rate,the surgery which after surgery hemorrhage quantities,leaks attracts the colporrhagia time and the illness complication formation rate,thus compares two surgeries methods the surgery quality.

目的：探讨可视无痛人工流产术与单纯无痛人工流产术的手术质量是否不同。方法：纳入符合标准的125例门诊病人，随机分为对照组和试验组。对照组采用单纯无痛人工流产术，试验组采用可视无痛人工流产术，观察并记录两组病人在手术中的出血量、漏吸的发生率、手术后阴道出血时间及并发症的发生率，从而比较两种手术方法的手术质量。

In contrast to conventional methods, a novel cell sheet-based technique has been proposed .This technique intend to achieve high cell density and function by layering confluent viable cell sheets without the use of acellular scaffolds that limit mass transport. Using thermo-sensitive cell culture surfaces, confluently cultured cells spontaneously detach as intact sheets simply by reducing the culture temperature.

与传统方法相比，有学者提出了一种新的以细胞层为基础的组织工程技术，这种技术作为一种新的选择，利用温敏性培养皿，经简单的温度变化，可以非损伤性的一起获得完整的培养细胞层及其所沉积的细胞外基质，从而避免了使用蛋白水解酶。

The pressure maintaining coring technique is a coring technique that can keep or be close to formation pressure.

美国最先在1939年提出保压取心技术，经过40多年的研究改进，于1982年投入生产使用。

A lot of technology experiments have been done about the manufacture technique, the influence on the micro-structure resulted from the (311) rapidly corrodible plane is founded and the compensation technique is studied.

最后对系统进行了大量的特性实验，这些实验不但充分证明了以上理论研究的正确性，而且为微机电谐振阵列嗅觉系统的实用化开发提供了宝贵的数据。

The hotspots about RNA silencing and the methods of this technique used in plants according to its durational differences were introduced. The current status and prospective of the technique in the areas of plant functional genomics studies, improvement of crop quality, and plant bre...

文章对RNA沉默研究中的几个热点问题进行了介绍，按RNA沉默持续时间的不同对其在植物中应用的方法进行了分析，并重点论述了RNA沉默在植物功能基因组研究、作物品质改良以及抗病毒植物培育等方面的应用及其发展前景。

The changes of the HAF after liver injury may be related to the volume of blood loss, the intraabdominal pressure and the pneumoperitoneum duration.

肝撞击伤伴失血后肝动脉血流量的变化与失血量、气腹压力以及气腹持续时间等有密切联系。

Reneging on our commitments to the world's poor cannot be an austerity measure, she said.

放弃我们给世界上的穷国的承诺不能成为一种紧缩措施。