查询词典 technical knockout

The choosing of the material and staining of Golgi body and making section;2. To determine the subcellular localization of the encoded protein of a transcriptionally up-regulated gene pdd87in the pten knockout mice,fusion protein expression vector pEGFP-C 1 -pdd87was constructed and transfected into NIH3T3cells and cells were stained with BODIPY TR C 5 -Ceramide to display Golgi body .

为了明确pten敲除小鼠中转录上调基因pdd87编码蛋白在细胞中的定位，构建PDD87与绿色荧光蛋白的融合蛋白表达载体pEGFP-C1-pdd87，利用Lipofectamine2000转染该载体于体外培养的NIH3T3细胞中，采用高尔基体特异探针BODIPYTRC5-Ceramide染色显示高尔基体，激光共聚焦显微镜下观察到PDD87-GFP融合蛋白定位于高尔基体，提示pdd87基因编码的蛋白定位于高尔基体。

Kim Building 15C, a registered capital of five million, have a professional and experienced sales engineers and technical support team of engineers to provide clients with the most perfect technical support services supported by the more than 100 professionals.

深圳市越腾科技发展有限公司，位于深圳市深南大道6019号金润大厦15C，注册资金500万，拥有专业资深的销售工程师和技术支持工程师团队，为客户提供最完善的技术支持服务支持，各项专业人士100多人。

Organize people to draw up quality inspection and acceptance standard based on the technical standard from technical department.

根据研发部的产品技术标准，负责组织产品质量验收标准的制定。

Our products approved as high quality products by Jiangsu province and also the advanced technical certificate by the city,province and also the government. Developed in 2003 for Fully automatic accommodation ladder system for large oil vessel (over 160,000DWT) have been approved as high technical product in 2004 by Jiangsu province.

产品先后获江苏省优质产品称号和国家、省、市科技进步奖，2003年开发生产的"大型油轮（16万吨以上）全自动舷梯系统"被江苏省认定为2004年度高新技术产品，并立为2005年度江苏省火炬计划项目，其关键零部件获国家多项"实用新型专利"，产品自动化程度及质量深受用户青睐。

Company has strong technical strength, technical staff accounted for more than 50%.

公司技术力量雄厚，技术人员占50%以上。

Is the production of mineral wool acoustic board in China for large enterprises, our advanced technology, sophisticated equipment, technical skills, management norms, the product of advanced technical indicators.

欧亚装饰材料有限公司是我国生产矿棉吸声板的大型企业，本厂工艺先进、设备精良、技术精湛、管理规范、产品技术指标先进。

One, the integral sex action of system of integrated traffic information is told from technical level, system of integrated traffic information is wait to carry a system to undertake transforming to traditional traffic through using technology of IT, communication, control technology, the modern science and technology that uses these technical delegates undertakes control and administrative to traffic system.

一、综合交通信息系统的整体性作用从技术层面上来讲，综合交通信息系统是通过采用信息技术、通信技术、控制技术等对传统交通运输系统进行改造，并运用这些技术代表的现代科技对交通系统进行控制和治理。

According to the present situations of the two production lines and the temperature needed by the producing technics of the heat-exchanger , using the numerical simulation with the Airpark software to analyze the reason why the cooling system can not meet the technicals requirement; with the heat transfer theory computation based on the heat transfer principle, the technical parameters of the cooling sect refer to the system technic and configuration are obtained. That are made for reasonable organization of the airflow of the cooling system and the new cooling equipment achieving the technical requirement. At the same time, it will ease the thermal pollution of the circumjacent environment and improve IAQ of the workshop

根据某厂两条换热器生产流水线的现状和冷却降温的工艺要求，用Airpark 软件进行数值模拟，分析了冷却系统无法满足工艺要求的原因；运用传热学原理进行了设备与环境的传热理论分析和计算，分析了冷却系统无法满足工艺要求的原因，得出了改造所需要的技术与结构参数，并对冷却系统进行合理布局与气流组织，使新的冷却系统发挥了应有的作用，满足了生产工艺的要求，同时也减轻了生产环境的热污染，提高了环境的空气品质。

More than 400 employees, including engineering and technical personnel more than 60 people, intermediate technical staff over 36 people, an area of 30,000 square meters, all the modern equipment more than 100 Taiwan.

现有职工400余人，其中工程技术人员60余人，中级以上技术人员36人，占地30000平方米，各种现代化设备100余台。

Companies with annual technical support hotline for customers with all-weather telephone counselling service and timely on-site technical support.

公司常年设有技术支持热线，能为客户提供全天候的电话咨询服务和及时的现场技术支持。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。