查询词典 teasy

The genome DNA was extracted from the red blood cells or blood of the infected sheep and goats which include as isolates of ovine Babesia from these different geographic areas. The gene specific primers were devised according to the pubulished 18srRNA sequence at GenBank. The target DNA segment was amplified by polymerase chain reaction, the PCR product was ligated into PGEM-Teasy vector.

用分离于四个地区的羊的巴贝斯虫感染实验动物，自感染羊的红细胞或全血中提取基因组DNA，根据GenBank中已发表的巴贝斯虫18S rRNA序列设计两对羊巴贝斯虫特异性引物，通过PCR扩增目的基因片段，将扩增产物连接到PGEM-Teasy载体中，经酶切鉴定，PCR分析，进行序列测定，结果显示羊巴贝斯虫四个分离株的18S rRNA基因大小为1800bp左右。

These quick and easy teasy highlights add just the right amount of beautiful diffused color, perfect for yuletide festivities and holiday cheer!

这些方便快捷teasy突出补充一点，美丽的扩散颜色，圣诞季节节日和假日欢呼完善适量！

Dwelling on these isn'teasy, but it's a vital first step to resolving them.

总想着这些问题是不好受的，但这是解决问题很重要的第一步。

Cute as you are, though sometimes teasy and hard te becoaxed,you are like a doll in my eyes.

你是个可爱的宝贝，虽然经常惹我生气，而且还要哄很久，但是还算是个乖宝宝。

Subsequently, a l kb fragment of HCCR-2 encoding region was amplified by RT-PCR and cloned into Promega TEasy vector.

测序证实无误后，以HCCR-2／T载体为模板，再通过PCR克隆出HCCR-2的编码区序列，将其连接到pIRES2-EGFP真核表达载体，并通过测序获得证实。

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna，利用rt pcr方法，先克隆出一包括hccr 2编码区的长约1 kb的片段，并将其与t载体连接并转化，测序证实无误后，以hccr 2/t载体为模板，再通过pcr克隆出hccr 2的编码区序列，将其连接到pires2 egfp真核表达载体，并通过测序获得证实。

Methods The hTERT promoter as E1A and E1Bp gene were amplified by PCR; the GFP gene and GM-CSF were also amplified by PCR, and the products were subcloned into Teasy plasmid in certain order to generate pSh-GFP-SV55K plasmid.

采用PCR的方法克隆腺病毒E1区基因(E1A， E1B55K)、EIB的启动子(E1Bp)以及人端粒酶逆转录酶亚基启动子，以及2个目的基因：人GM-CSF和GFP(Green Fluorescence Protein， GFP)。

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA，利用RTPCR方法，先克隆出一包括HCCR2编码区的长约1 kb的片段，并将其与T载体连接并转化，测序证实无误后，以HCCR2/T载体为模板，再通过PCR克隆出HCCR2的编码区序列，将其连接到pIRES2EGFP真核表达载体，并通过测序获得证实。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

Some questions asked by the adjudicators in one English Speaking Contest still run upon me very often.

我参加了一场以"我心中的奥运会"为主题的英语演讲竞赛，其间评判提的几个问题时时萦绕我的心头。

He called InTERActive our most profitable release of the year.

他呼吁互动的我们的最有利可图的释放一年。