查询词典 superfusion

The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 24-72 h in DMEM plus 10 % calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within

结果表明：以1 mL/h 的速度灌流培养的奶牛乳腺组织在DMEM+ 10 %小牛血清培养基中24~72 h 内保持良好的组织活性和超微结构，贴壁培养的奶牛乳腺组织在DMEM+ 10%小牛血清的培养基中48~108 h 内保持良好的组织活性和超微结构，两种培养方法各有优缺点。

Under the superfusion of low potassium Tyrode's solution, EAD were relatively easy to occur in both mouse atrial and ventricular fibers, suggesting the close relation between the potassium currents and the generation of EAD.

我室以往的工作表明：小鼠心肌是研究EAD的理想标本。既使轻微的低钾（3mM 〓）条件也足以诱发小鼠心房肌和心室肌的EAD（尽管二者难易度上存在差别），提示小鼠心肌细胞上的钾电流与EAD的发生关系密切。

The extent of inhibition of depolarization by superfusion of ethanol for 5 minutes was 41.8±3.3 ％(n=17). The addition of 5 nM and 20 nM KT5720 (a selective protein kinase A inhibitor) and 250 μM H9 dihydrochloride (a non-selective protein kinase inhibitor), into the intracellular solution significantly attenuated the inhibitory effects of ethanol.

当分别将5 nM或20 nM KT5720（选择性蛋白质激酶A抑制剂）或250 μM H9 dihydrochoride（非选择性蛋白质激酶抑制剂）置入细胞内液时，乙醇的抑制作用明显被减弱；但乙醇的抑制作用不受到5 nM或20 nM calyculin A（磷酸酶1/2A抑制剂）的影响。

Ethanol inhibition was not affected by 5 nM and 20 nM calyculin A (a phosphatase 1/2A inhibitor). Prior superfusion of 5 nM and 20 nM KT5720 also attenuated ethanol inhibition of NMDA-mediated depolarization. 1 μM and 3 μM chelerythrine, a selective protein kinase C inhibitor or 20 nM calyculin A did not affect ethanol inhibition of NMDA-induced response.

当以表面灌流方式，事先给予5 nM或20 nM KT5720时，同样可以减少乙醇抑制NMDA引发的去极化作用；分别给予1 μM或3 μM chelerythrine或20 nM calyculin A则不会影响乙醇抑制NMDA所引发的反应。

L-glutamate produced a persistent elevation of ［Ca2+］i . We measured a detectable small elevation of ［Ca2+］i after starting superfusion of ischemia-simulating medium.

激光扫描共聚焦显微镜（Laser Scanning Confocal Microscopy， LSCM）的问世，才使单细胞内Ca2+瞬间时空动态变化的检测成为可能。

The inward current in OHCs was totally inhibited after the superfusion of 100μmolIL and 200μmo1/L linopirdine respectively. The outward rectifier K(superscript +) current I(subscript K was the dominant K(superscript +) current in the whole cell currents in Deiters' cells. In the presence of 200μmol/L linopirdine, the current was not significantly affected.

在细胞外液中，加入100μmol/L利诺吡啶后，OHCs中的四乙基二乙胺敏感的钾电流峰电流成分被抑制，稳态电流幅值减小，且电流的失活时间常数明显延长；在细胞外液中加入100μmol/L利诺吡吮后，OHCs的内向性钾电流I被完全抑制；而细胞外液中利诺吡啶终浓度为200μmol/L时，Deiters 细胞的外向整流性钾电流幅值无明显变化。

After the application of 100μ M linopirdine to OHCs, the peak K+ current was reversibly blocked and the late K+ current was partly reduced. In addition, the decay time constant of the TEA-sensitive K+ current was prolonged in the presence of 100μ M linopirdine. The inward current in OHCs was totally inhibited after the superfusion of 100μ M and 200μ M linopirdine respectively.

结果发现，在正常细胞外液中，单离外毛细胞可记录到四乙基二乙胺敏感的外向性钾电流和静息膜电位附近激活的内向性钾电流（ the K+ current activated at negative potential， IKn）两种钾电流，而单离Deiters细胞中只记录到外向整流性钾电流。

Establishment of a bovine mammary tissue culture system Bovine mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation.

1奶牛乳腺组织体外培养方法的建立采用组织灌流和组织块贴壁法体外培养奶牛乳腺组织，通过LDH活力测定、台盼兰染色、琼脂糖凝胶电泳、透射电镜技术比较两种培养方法对奶牛乳腺组织活性及组织超微结构的影响，建立奶牛乳腺组织的培养体系。

By superfusion of ET-1,the hypoxia-induced decrease in RPF was remarkably potentiated and the occurrence of pacemaker arrest was shifted to an earlier time. The h3,poxia-induced effects could be effectively attenuated after pretreatment with BQ-123,implying the role of endogenous ET-1 release in hypoxia-induced effects.

ET-1可显著加重缺氧所致的RPF降低，使起搏细胞停跳时间前移；而以ET*受体阻断剂BQ-123预处理窦房结标本后，则能有效地缓解缺氧对起搏细胞的效应，提示内源性ET-1的释放在缺氧效应中的作用。

Respiratory-related burst activities were recorded from hypoglossal nerve rootlets before and during superfusion of theslice preparation with L-Arginine, sodium nitroprusside or 7-nitro indazole (7-NI, an in-hibitor of NO synthase).

同时保留舌下神经根，用改良Kreb's液灌流脑片并记录与之相连的舌下神经根呼吸节律性放电(respiratory rhmical dischargeactivity，RRDA)，在灌流液中分别给予不同浓度的NO供体硝普钠(sodium nitroprusside，SNP)，NO合成前体L-精氨酸(L-Arrginine，L-Arg)以及神经元型一氧化氮合酶(neuronal nitric oxide synthase，nNOS)特异性抑制剂7-nitro indazole(7-MI)，观察其对RRDA的影响。

The teacher likes the honeymouthed little girl very much.

老师很喜欢这个嘴甜的小姑娘。

Mr. Notker Bien's interests are traveling, spending quality time with the family and long-distance-running.

诺特卡·柏恩先生热爱旅游，长跑，以及和家人一起共度美好时光。