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A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

Strong leadership skills and ability to operate and successfully arbitrate in a complex international, multicultural environment.

强有力的领导艺术，能在复杂的、国际化的，多元化的环境中做出正确判断。

It is the first time that no additional anode was adopted in the magnetic macroparticles filter. At the same time the auto-re-igniting system was designed for vacuum cathodic arc deposition instrument. It ignites the arc with an electronic component with high voltage and can auto distinguish weather the arc dies out or is on during the deposition via a main power supply feedback unit. The energy for igniting the arc is very less and thus can successfully prevent the film being contaminated.

设计制造的电子式真空阴极弧离子镀引弧器成功地实现了高压脉冲引弧，可以多次重复使用，所用能量较小，可以有效防止对所镀膜的污染，引弧可靠，能够自动识别电弧是否引燃，能够在熄弧后自动重新引弧，重新引弧的速度快，有效防止了熄弧引起的气压波动，使镀膜过程中气压非常稳定。

The integration of welding machine and arc welding robot system is implemented successfully.

成功的实现了该电焊机与弧焊机器人系统的集成。

Designed and produced by Argosy Technologies in Russia, more than 600 R-AT-MM upgrade systems have been installed and are successfully being operated by the major Russian oil and gas companies.

由俄罗斯Argosy 技术公司设计和生产的600多套R-AT-MM升级系统已经安装并成功地运行于俄罗斯主要的石油和天然气公司里面。

By applying the difference arithmetic to solve the differential vibration model of the single-degree-of-freedom,the acceleration responses of the vibrated bevel wheel was calculated.The bevel wheel contact fault was simulated by taking it as the impulsion loading in local point.By using techniques of wavelet de-noising and demodulated resonance,gear contact character information was picked up successfully.

从齿轮的单自由度振动模型出发，运用差分算法对模型进行求解，得到斜齿轮的振动加速度响应，将齿面接触型故障等效为在局部点突加一个冲击载荷，对齿轮齿面接触型故障进行了仿真，利用小波降噪和共振解调技术分别对仿真的染噪振动加速度信号进行处理，成功地提取了轮齿接触故障的信息。

SBR can be successfully used in the analysis of trihedral corner reflectors, the improved arithmetic enhances the speed of calculation greatly.

结论将射线跟踪法应用于三面角反射器的后向散射场的研究中；改进后的算法不但计算准确而且运算速度也有所提高

It should improve both our comprehension of coagulation abnormalities in sepsis and how those abnormalities link up to inflammation, but it may also add to the therapeutic armamentarium with which sepsis, severe sepsis, and septic shock can be more successfully treated.

它将使我们能够更加深入的理解脓毒症时的凝集异常以及这些异常同炎症反应的关系，同时，此观点融入也将使脓毒症、严重脓毒症和脓毒性休克的治疗获得更大成功。

The lets Internet successfully open mind of Facebook was ascended downstage, the author reviewed a few successful famous websites and product is the open mode operation of type of ant powerful army almost, and the success of Firefox also is its a source, open result.

Facebook 的成功让互联网的开放精神登上了前台，笔者反思了几个成功的著名网站和产品几乎都是蚂蚁雄兵式的开放式运营，而Firefox的成功也是其开源、开放的结果。

Suspension sampling technique was applied to flame atomic absorption spectrometry and was successfully used to determine calcium, magnesium in arrowroot flour .

将悬浮液进样技术应用于火焰原子吸收光谱法，测定了藕粉中的钙、镁含量。

The system can be widely used in belt weighing and batching process control,etc.

介绍了基于智能模糊控制的嵌入式配料系统的设计方法，对模糊PID控制过程和系统调零作了重点的讨论，同时给出了PID控制的程序流程图和系统定长调零子程序。

"I dunno," she answered,"but if you show me aroun', I'll recognize it from the lighthouse just outside the porthole."

"我不知道，"她回答说，"如果你能带我转转，我能认出来的，因为灯塔就在舷窗外。"