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The results showed that: the explants of strawberry leaves were rather sensitive to kanamycin, and the corresponding concentration of 20 mg/L could substantially inhibit the growth of non-transformed tisse and be used to screen the leaves of strawberry after transformation. Timentin at the concentration of 300 mg/L could be used to eliminate Agrobacterium tumefaciens and had minor impact on the growth of the explants. The preculture time of 2-4 days was beneficial to improve transformation frequency. Co-culture for 2-3 days not only had higher gus transient expression but also avoid the over-growth of agrobacterium. The bacterial concentration of OD(subscript 600nm) 0.4 was the best inoculum density and 10 minutes was the best inoculation time.

结果表明:草毒叶片外植体对卡那霉素较为敏感,适宜的筛选浓度为20mg/L,可明显抑制非转化组织的生长;300mg/L的羧吩青霉素可有效抑菌,对外植体生长影响也较小;预培养2-4d有利于转化;共培养2-3d有利于提高转化频率并避免了农杆菌的过度生长;OD(下标 600nm)值为0.4的菌液侵染10min效果最佳。

METHODS: Titanium nails (1.5 mm in diameter) were separately inserted on two sides of left premaxillary suture in rabbits. A self-made distraction device was used. Gelatum-like substance [V(subscript platelet-rich plasma):V=9:1] was injected into the left premaxillary suture of experimental group rabbits immediately prior to distraction.

分别在家兔左侧前颌缝的两侧置入钛钉(直径1.5mm),戴自制前牵引装置,试验组在牵张开始时,注射V∶V=9:1混合的凝胶样物质至左侧前颌缝内。

After coating the solutions on the substrates, the precursor films will go through a heat treatment to pyrolyze the polymer and form solid films, In this paper, we show some representative oxide and nitride films grown by PAD, including simple oxide/nitride such as TiO2, GaN, A1N and complex oxide/nitride such asTiO3 and Ti(subscript 1-x)AlN.

该文介绍了使用该方法制备的一些具有代表性的氧化物和氮化物薄膜,包括简单氧化物/氮化物,如TiO2、GaN和AlN等,复杂氧化物/氮化物如(Ba,SrTiO3、Ti(下标 1-x)AlN等。

The influence of chain extender kind, content and reactive processing condition on molecular weight (averageM(subscript η) of recycled PET was studied carefully based on chain extenders of 2, 2'-Bis(2-oxazoline), Pyromellitic dianhydride and the mixture of the both during reactive extrusion processing.

分别使用双恶唑啉、均苯四酐及两者的混合物作为回收PET瓶的扩链剂,研究扩链剂的种类、扩链剂用量和反应挤出机加工工艺条件对PET产品相对分子质量

The closed system is shown to be quadratically stable and the H(subscript ∞) performance can arbitrarily approach to that achieved by state feedback.

结果证明,闭环系统可以保持二次容许,且H鲁棒性能可任意过近原状态反馈系统。

Methods This study contained a total of 16 culture groups, including one group as control and 5 groups treated by 5 drugs (calcium L-threonate, sodium L-threonate, alendronate, 17β-estradiol and calcium gluconate) each at the final concentrations of 10^(-9)mol/L, 10^(-7)mol/L, 10^(-5)mo1/L respectively. After 7 days, eight bone slices of every group were stained with toluidine blue and the areas of resorptive pits were analyzed under light microscope; the concentrations of C-telopeptide of type Ⅰ collagen CT(subscript x or Crosslaps in culture supernatants were measured by ELISA.

制备骨磨片,培养OC,并分别加入高中低浓度10^(-5、10^(-7)、10^(-9)mol/L的L-苏糖酸钙、L-苏糖酸钠、阿仑麟酸钠、17β-雌二醇、葡萄糖酸钙,实验设以上各浓度组及对照组(共16组,每组n=8),甲苯胺蓝染色一光镜观察分析骨片上骨吸收陷窝面积,ELISA测定上清液Ⅰ型胶原C-末端肽(CTx或Crosslaps)浓度。

The result shows that the purification efficiencies of ammonia-nitrogen and ortho-phosphate by immobilized algae in a stationary phase are higher than that in an exponential phase; the starvation treatment increases the removing rates of ammonia-nitrogen and ortho-phosphate, cells of Scenedesmus starved for 48 hours can obtain higher NF4(superscript +)-N and PO(superscript 3- subscript 4)-P removal efficiencies than starved for 48, 72, 24 and 0 hours respectively.

结果表明:静止初期藻细胞对NH4-N和PO(上标 3-下标 4)-P去除率比指数末期高,处理5d后,静止初期细胞对NH4-N和PO(上标 3-下标 4)-P的去除率分别为76.7%及91.7%,指数末期细胞氮、磷的去除率分别为75.4%和88.7%;饥饿处理可明显提高藻细胞对NH4-N和PO(上标 3-下标 4)-P的去除效率,藻细胞饥饿时间对NH4-N和PO(上标 3-下标 4)-P去除率的影响大小依次为48h,72h,24h,0h。

Under inverted microscope, stereognosis of neurons died down, there were blank bulb in the neurons, dark particle were seen in the neuritis; the result of MTT test showed that the survival rate of neurons was obviously decreased. Res 40μmol/L increased the survival rate of neurons. Aβ(subscript 25~35) dose-dependently increased the level of MDA, decreased the level of GSH. Res dose-dependently increased the level of GSH, decreased the level of MDA.

结果:Aβ(下标 25~35)在终浓度为2μmol/L时神经元形态有明显的变化,细胞立体感减弱,胞体内空泡形成,细胞突起内可见黑色颗粒状物;细胞的存活能力明显下降,与对照组相比较有显著性差异(P.05);Res在终浓度40μmol/L时,能明显提高细胞的存活能力。Aβ(下标 25~35)可以明显升高细胞培养上清液中MDA含量,降低GSH的含量;Res可使细胞培养上清液中MDA含量下降,GSH含量增加。

It showed that, compared with other catalysts, SO(superscript 2- subscript 4)/TiO2-ZrO2 was more active to bring about more than 95% conversion rate of stillingia oil under the transesterification conditions of 150℃, molar ratio of methanol to oil 12:1, catalyst amount 5% and reaction time 6h.

结果表明,催化剂SO(上标 2-下标 4)/TiO2-ZrO2表现出了较高的催化活性,SO(上标 2-下标 4)/TiO2-ZrO2催化的最佳酯交换反应条件为:反应温度150℃,醇油摩尔比12:1,催化剂用量5%,反应时间6h,此时乌桕籽油的醋化率超过95%,且催化剂重复和再生使用效果良好。

Ma 5(subscript 1-4) submember is an asymmetric long-term base level cycle which can be divided into five middle-term base level cycles and more than 26 short-term base level cycles.

马五(下标 1-4)亚段构成了一个长期基准面下降半旋回,其又可划分为5个中期基准面沉积旋回及多于26个短期基准面沉积旋回。

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