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Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Though can not accurate induce neural stem cells specific orientation differentiation yet,to accompany the unceasing deepen recognize of convection mechanisms in cells' signal convection and discovery of new cell facter,new chemo-signal, Homo sapiens would solve the question of orientation differentiation from totipotential stem cell,multipotential stem cell to neural stem cell, orientation nerver ancestor cell then special tipe neural cell, implementation functional reconstruction betwen neural cell,for ultimate solve the proble of neural regeneration developing the way.

尽管目前尚不能准确有效的诱导神经干细胞特异性定向分化为某种特定的神经元,但随着人们对细胞间信号传递机制认识的不断加深和新的细胞因子、化学信号的不断发现,人类将能够解决从全能干细胞、多能干细胞到神经干细胞、定向神经祖细胞直至特异类型神经细胞的定向分化,完成神经元之间的功能重建,为最终解决中枢神经再生这一难题而开辟道路。

The animal model of this method system have, the characteristics of the high fat, high leptin, high Ins, and match with the characteristic of clinical and the pathologic of simple obesity. This studies showed: The acupuncture can obviously reduce the vaule of intaking food and water of the fat rats, and lower the body weight and fat; The acupuncture can urge the diameter, area, physical volume of white fat cell contract, Brown fat tissue of fat rat group surroundings vascular containing large quantity of white fat cell, the acupuncture group was less. this showed: The acupuncture enhanced the white fat cell metabolism, and promotesed the white fat cell to convert to brown fat cell; The frequency of spontaneous electric of nerve cell of pvn of the fat rat is high, the acupuncture counld depress the spontaneous electric of nerve cell of pvn, This showed: the acupuncture can depress the appetite passing to adjust function of pvn nerve; The monoaminergic neurotransmitter of inside of the fat rat brain is disfunction, the acupuncture can rectify this disfunction, and adjust level of 5-HT, and affect appetite, and improve metabolism; The level of leptin and Ins of fat rat increased high, The level of it of acupuncture group decreased, compared with the fat group p.001, This showed: the fat rats have the defeat of leptin and Ins in the body, The acupuncture can lowering this defeat, The level of leptin and Ins of the fat rat in the brain is less than the normal group, The level of leptin of the acupuncture group is high than the fat group, This showed: the acupuncture can increase the transportation of leptin from blood to brain.

本方法所制肥胖大鼠模型具有高体重、高脂、高leptin和高Ins血症的特点,符合人类单纯性肥胖病的临床和病理特征;实验研究显示:针灸能明显减少肥胖大鼠的摄水、摄食量及降低其体重、体脂;针灸能促使白色脂肪细胞直径、面积、体积缩小,减少肥胖大鼠棕色脂肪组织血管周围白色脂肪细胞数量,显示:针灸增强了白色脂肪细胞的代谢,促进了白色脂肪细胞向棕色脂肪细胞的转化:肥胖大鼠PVN神经细胞自发放电频率增加,脑内单胺递质紊乱,针灸通过抑制PVN亢奋的自发放电,调整5-HT水平,达到影响食欲,改善代谢的作用;肥胖大鼠血中leptin及Ins水平增高,与正常组大鼠比有明显差异,提示大鼠体内存在leptin及Ins抵抗,针灸后肥胖大鼠随着体重体脂的下降,血中leptin、Ins水平降低,与肥胖组比均有显著性差异,而针灸后大鼠脑内leptin增加,与肥胖组比P.05,提示:针灸具有改善leptin、Ins抵抗及增加leptin脑内转运的作用。

Its main features are: algae body as a single cell or even into a variety of groups; cell walls composed of silica and pectin, but also by the structure of the shell and the shell under the combined set of the Cheng, vertical section view was "soap-box"-shaped; in the upper and lower shell surface of the shell are patterns arranged in patterns on both sides of the main mode of symmetry and radial symmetry is divided into two categories, for the classification of the most important basis; be able to campaign Type in the shell has a shell side seam, can not be changed no shell seam types; pigment body sheet or ribbon, 1 or 2, or for multiple small discoid, yellow green or brown, containing chlorophyll a, c, also contains fucoxanthin and silicon A flavin and other photosynthetic pigments; storage material mainly for oil droplets; reproduction mainly for cell division and cell division in diatoms obvious features are: each cell division, resulting from two sub-cells,, only one and the mother cell and so big, the other one is smaller.

其主要特征是:藻体为单细胞或连成各式群体;细胞壁由硅质和果胶质组成,而且在结构上都是由上壳和下壳套合而成,纵断面观呈"肥皂盒"形;在上下壳的壳面上有花纹,花纹排列的方式主要分为两侧对称和辐射对称两大类,为分类上的最重要依据;能运动的种类在壳面都有壳缝,不能动的种类均无壳缝;色素体片状或带状,1个或2个,或为多个小盘状,黄绿色或黄褐色,含叶绿素a、c,还含有墨角藻黄素和硅甲黄素等光合色素;贮藏物质主要为油滴;繁殖方式主要为细胞分裂,硅藻的细胞分裂的明显特点是:每次细胞分裂所产生的2个子细胞中,仅有1个和母细胞等大,另1个则稍小。

Monocellular Raman spectrums of normal liver cell line (LO2) and carcinoma liver cell line (SMMC-7721) were studied with laser tweezers Raman spectrums system and each cell was investigated in three different points, the result of which showed; significant differences of average Raman spectrums were found between the normal cell and the carcinoma one; And, the spectra line of carcinoma cell became weak at the whole; Additionally, the spectral peak intensity ratio of normal cell at 1658cm^(-1) and 1450cm^(-1) was 0.63 while that of carcinoma was 0.99; Moreover, the structure and the amount of protein; nucleic acid, lipids and other such molecules had also changed.

本工作利用激光镊子拉曼光谱系统研究了人正常肝细胞株(LO2)和肝癌细胞株(SMMC-7721)的单细胞拉曼光谱,对于每个细胞在不同部位测三个点。实验结果显示:正常细胞和癌细胞的平均拉曼光谱存在显著差异;癌细胞谱线强度整体变弱;正常细胞的1658cm^(-1)处峰和1450cm^(-1)处峰的强度比值为0.63,癌细胞的为0.99;癌细胞的核酸、蛋白质、脂类等重要生物分子在结构或含量上都发生了不同的改变。

Furthermore,the changes of cell organelles realized different cell functions, which fiber for mechanical support,parenchyma cell for preservation and vessel for transportation.3.FE-SEM and DCR technique revealed the arrangements of CMfs on cell wall of fiber, parenchyma cell and vessel.Firstly,fiber cell formed multilamellate concentric structure with thin and thick lamellae alternate.

纤维、基本薄壁组织细胞、导管分子的终端分化就是PCD;并且PCD过程是一个具有较长周期的过程,周期长短比较为基本薄壁组织细胞>纤维>导管分子;三类细胞最后的凋亡决定于细胞内原生质体完全降解的时刻。

Its main features are: algae body as a single cell or even into a variety of groups; cell walls composed of silica and pectin, but also by the structure of the shell and the shell under the combined set of the Cheng, vertical section view was "soap-box"-shaped; in the upper and lower shell surface of the shell are patterns arranged in patterns on both sides of the main mode of symmetry and radial symmetry is divided into two categories, for the classification of the most important basis; be able to campaign Type in the shell has a shell side seam, can not be changed no shell seam types; pigment body sheet or ribbon, 1 or 2, or for multiple small discoid, yellow green or brown, containing chlorophyll a, c, also contains fucoxanthin and silicon A flavin and other photosynthetic pigments; storage material mainly for oil droplets; reproduction mainly for cell division and cell division in diatoms obvious features are: each cell division, resulting from two sub-cells,, only one and the mother cell and so big, the other one is smaller.

其主要特征是:藻体为单细胞或连成各式群体;细胞壁由硅质和果胶质组成,而且在结构上都是由上壳和下壳套合而成,纵断面观呈&肥皂盒&形;在上下壳的壳面上有花纹,花纹排列的方式主要分为两侧对称和辐射对称两大类,为分类上的最重要依据;能运动的种类在壳面都有壳缝,不能动的种类均无壳缝;色素体片状或带状,1个或2个,或为多个小盘状,黄绿色或黄褐色,含叶绿素a、c,还含有墨角藻黄素和硅甲黄素等光合色素;贮藏物质主要为油滴;繁殖方式主要为细胞分裂,硅藻的细胞分裂的明显特点是:每次细胞分裂所产生的2个子细胞中,仅有1个和母细胞等大,另1个则稍小。

Based on a variety of books and materials, foreign or domestic, this thesis deals with the recent development and application, the past, present and future for solar energy and solar cells, analyzes the great senses of solar energy and solar cells for the development of economic, and analyzes their structure photovoltaic effect and conservation efficiency. The writer also elaborates the working principles and application characteristic of solar cell, various factors that may lead to bad conservation efficiency of solar cell. Based on the the working principles and application characteristic of solar cell, emphasizes on some ways of improving photovoltaic conservation efficiency for solar cell, for example: the appropriate thickness of solar cell, the low resistance, the phalanx of solar cell, the film of reduce the reflect of solar rays, and so on, and designs simply the feasible way, then give out the CAD frame chart .

本文是在收集了大量的国内外图书资料的基础上,对太阳能和太阳能电池的广泛应用领域、过去、现在和未来进行了探讨,分析了太阳能和太阳能电池的对我国经济发展的重大意义,阐述了太阳能电池的工作原理和应用特性,论述了影响太阳能电池光电转换效率的因素,重点介绍了几种提高太阳能电池光电转换效率的方法,例如:合适的太阳能电池厚度、降低太阳能电池的串联电阻、采用太阳能电池方阵、采用光伏发电自动跟踪系统、采用减反射膜和折面型抛物柱太阳能聚光器,并对以上可行的方法进行了简单综合设计,实现了 CAD 框图。

CD8~+ cell is the main T lymphocyte subset in spleen, and B lymphocyte mainly is IgG~+ cell, moreover the amount of these B lymphocytes could exceed CD3~+ T lymphocyte subset after 7 days. CD8~+ cell is the main T lymphocyte subset in tonsil of appendix, and B lymphocyte is IgM~+ cell, and the amount could exceed CD3~+ T lymphocytes after 35 days. After 21 days, B lymphocytes in esophago tonsil are the main IgA~+ cells and the amount exceeds CD3~+ lymphocytes. The amount of CD4~+ lymphocytes is more than CD8+ lymphocytes.4. CD3~+、CD4~+ and CD8~+ T lymphocytes in spleen mainly distribute in periarterial lymphoid sheath. However IgM~+、IgG~+ and IgA~+ cells mainly distribute in ellipsoid periarterial lymphoid sheath and germinal center. T lymphocytes in appendix tonsil mainly distribute in middle and inferior part of mucous and the B lymphocytes mainly in middle and mucous between 4~7 days. Whereafter T, B lymphocytes equably distribute in mucous. CD4~+ cells arrange tightly and mainly occupy the central part in aggregates of T lymphocytes in esophago tonsil and CD8~+ lymphocytes mainly distribute in periphery. Meanwhile B lymphocytes encircle the periphery of aggregates of T lymphocytes. The aggregates of B lymphocytes is mainly the germinal center with lots of IgM~+、IgG~+ and IgA~+ cells. Meanwihle T lymphocytes encircle the periphery of aggregates of B lymphocytes.5. There is an intimate relationship between the development of tissue structure of peripheral immune organs and lymphcytopoiesis. The maturation of tissue structure is stimulated by the immigration of lymphocytes and the mature tissue structure provides place where lymphocytes grow mature and functionate.

脾脏在21日龄时达到稳定,食管扁桃体和盲肠扁桃体均在35日龄时达到稳定;脾脏中T淋巴细胞亚群以CD8~+细胞为主,B淋巴细胞则以IgG~+细胞为主,并在7日龄后数量超过CD3~+T淋巴细胞;盲肠扁桃体中T淋巴细胞亚群以CD8~+细胞为主,B淋巴细胞以IgM~+细胞为主,并在35日龄后数量超过CD3~+T淋巴细胞;21日龄后,食管扁桃体中B淋巴细胞以IgA~+细胞为主,数量超过CD3~+细胞,CD4~+细胞的数量多于CD8~+细胞。4、在T、B淋巴细胞组织定位方面,脾脏中CD3~+、CD4~+和CD8~+T淋巴细胞主要分布在动脉周围淋巴鞘中,而IgM~+、IgG~+和IgA~+细胞主要分布在椭球周围淋巴鞘和生发中心中;4~7日龄时,盲肠扁桃体中T淋巴细胞主要分布在粘膜固有层的中下部区域,而B淋巴细胞则主要分布在中上部区域,随后各日龄T、B淋巴细胞均匀地分布在粘膜固有层中;在食管扁桃体的T淋巴细胞聚集物中,CD4~+细胞紧密排列,主要占据中央部位,CD8~+细胞主要散布在外周,同时B淋巴细胞又环绕在整个T淋巴细胞聚集物的外周;B淋巴细胞聚集物主要为生发中心,其中存在大量IgM~+、IgG~+和IgA~+细胞,同时T淋巴细胞又环绕在整个B淋巴细胞聚集物的外周。5、外周免疫器官的组织结构发育和淋巴细胞发生之间存在密切的关系,淋巴细胞迁入淋巴器官刺激组织结构的发育成熟,同时成熟的组织结构又为淋巴细胞发育成熟并行使功能活动提供场所。

We also analyze the mechanism of the initial zinc blende structure and wurtzite structure phase transition in II-VI and III-V compound materials. The results indicate that the transition of zinc blende structure to rocksalt structure due to "chair" structure transform, the transition of wurtzite structure to rocksalt structure due to "chair" and "boat" structure transform.

对II-VI族和III-V族初始相为闪锌矿或纤锌矿的压致结构相变机制进行的分析表明,闪锌矿结构到岩盐矿的相变是因为"椅式"结构的变化,纤锌矿结构到岩盐矿的相变是因为"椅式"和"船形"两种结构的变化。

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推荐网络例句

The role of the environment is very important.

的作用,环境是非常重要的。

At that time, the High Court of England and Wales Court of Queen's Bench president of William Murray, Lord Mansfield in June 22, 1772 ruling:"No matter then there is the inconvenience, but there must be a decision, I can not say that this case under the law in England are permitted or authorized; are so black should be released."

当时的英格兰及威尔士高等法院王座法庭院长William Murray, Lord Mansfield于1772年6月22日宣判:「无论有那麽不便,但总要有个决定,我不能说这件案在英格兰法律之下是准许或认可;所以黑人是应该被释放。

You can't walk into a store and take something out and then, 21 days later, if you decide to keep it, you pay the store!

你不可能去商店拿回一样东西,然后21天后,在决定留下它时,才付钱给商店。