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In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

我们用RT-PCR和整体原位杂交的方法研究了银鲫pou2基因在胚胎发育过程中的时空表达图式RT-PCR结果显示，银鲫pou2基因有母源转录本，其合子基因在高囊胚期强烈表达，在50%下包期和90%下包期也有高量的转录本，但在100%下包期表达量急剧降低，至体节期时已经完全检测不到其转录本胚胎整体原位杂交结果显示其母源转录本在所有的胚盘细胞中。

Up to now, the reports on the erythroblast differentiation regulatory factors are mainly focused on the early stage of erythroblast, the early erythroblasts are known to be the last stage of EPO dependant.

直至目前为止，文献报道的红系特异的作用因子主要集中于红系分化的早期阶段。

Up to now, the reports on the erythroblast differentiation ragulatoty factors are mainly focused on the early stage of erythroblast, the early erythroblasts are known to be the last stage of EPO dependant.

直至目前为止，文献报道的红系特异的作用因子主要集中于红系分化的早期阶段。

The optimum conditions were as follows: ethylamine was 370 mmol; n:n:n was 1.0:2.1:2.1; PEG-600 was 5 mol%; the first stage reaction was carried out at 42 ℃ for 6 h and the rsecond stage reaction was at 60 ℃ for 7 h.

较佳的反应条件为：乙胺370 mmol，n：n：n=1.0：2.1：2.1，PEG-6005 mol%，第一段于42℃反应6 h；第二段于60℃反应7 h，收率71.58%。

It employs the complex-valued step function to build neural model, then constructs network structure and decides the number of neurons; it adopts complex-valued Hebbian rules and inner product way to calculate the net weight matrix; then it analyzes the convergence of the network by taking advantage of the definition of energy function. In the image pre-processing stage, 2-dimensional Fourier Transform and Euler's formula are used, respectively, to transfer a gray-scaled image into phase information needed by the complex-valued neural network when stores the traffic sign. In the image post-processing stage, phase inverse transformation is used to change the phase image to gray-scaled image.

采用复数阶跃函数构建了神经元模型，建立了复数Hopfield神经网络结构，并且确定了网络中神经元的个数；借助复数Hebbian学习规则和复数内积法给出了网络权值的确定方法；借用复数网络能量函数的定义，说明了复数识别网络的收敛性；在前期的图像数据处理部分，分别使用离散二维傅里叶变换和欧拉公式，给出了将灰度图像数据转化为复数网络所需要的相位信息的方法，实现了网络对路牌的记忆存储；在后期的图像还原部分利用相位逆变换的方法，实现了相位图到灰度图转化。

This stage can last longer than the first euphoric stage—perhaps months.

这一阶段可以长于第一欣快阶段，或许几个月。

The fourth stage of the economic recession, and may even evolve into an economic crisis stage.

第四阶段，经济衰退，甚至可能演变为经济危机的阶段。

The linking of China's foreign exchange market went through two stages,that is, the stage of foreign exchange market of adjustment and the stage of foreign exchange market between banks.

我国的外汇市场经历了外汇调剂市场和银行之间的外汇市场两个发展阶段，形成具有一定规模的市场体系。

For multi-stage pump in series operation with the expeller seal combined with packing, the sealing water pressure should be: the lowest sealing water pressure of nth stage =∑Hi+0.7Hn, where n≥2

对于多级串联采用填料加付叶轮组合式密封，二级和二级以上轴封水压力一般为：第n级轴封水最低压力=∑Hi+0.7Hn，其中n≥2

We stopped by the old fashion ice cream parlor Manhattan Beach Creamery for some refreshment. The "No Shoes, No Shirt, No Problem" sign on the wall epitomizes the beach lifestyle.

我们去商店区的一家老式冰淇淋店坐坐，墙上挂著「没鞋，没上衣，没问题」的牌子完全捕捉了海滩生活的随性及风格。

"If therefore", argues Bellarmine we can offer our prayers and our satisfactions in behalf of those detained in purgatory, because we are members of the great body of Christ, why may not the Vicar of Christ apply to the same souls the superabundant satisfaction of Christ and his saints--of which he is the dispenser?

" "因此，如果"，认为贝拉明（德indulgentiis ，十四）"我们可以提供我们的祈祷和我们满意的代表那些被拘留在炼狱，因为我们是成员的伟大基督的奥体，为什麼可能不是牧师基督适用於同样的灵魂满意的过剩是耶稣和他的圣人-他是在配药？