查询词典 source free vector field

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

The System inset remote sense vector data and GIS vector data in drainage area into client programs programmed, realizing the application of remote sense vector data and GIS vector data in water resources management of Xinjiang Ma-na-si River irrigation area, and realizing the integrated management of the texts and maps of the water resources survey data, and spatial analysis and map edit functions of GIS as well. The system, which breaks away the platform of MapInfo, can run independently.

该系统将流域中的遥感栅格数据与GIS矢量数据集成嵌入到所编制的客户程序中，实现遥感数据和GIS数据在新疆玛纳斯河流域水资源管理中的应用，和水资源调查数据图文一体化的管理，同时具备了GIS的空间分析和图形编辑等功能，该系统能脱离Mapinfo软件平台独立运行。

The purpose of this article: to study the force-testing method of the side-kick in free-fighting, and develop the new field of three dimensional force-testing according to many experiments by means of three-dimensional force plate; to discover the kinematics rules and characteristics of the side-kick in free-fighting through the experimental study on the side-kick's biomechanics and kinematics characteristics of 18 excellent free-fighting athletics coming from Shandong Province, which will provide reliable kinematics indexes and parameters for diagnosing and improving the motion skills of free-fighting side kick,and for free-fighting teaching and training, further systemize and scientize the theory research of free-fighting motion.

研究目的：运用生物力学的研究方法，通过KISTLER 三维测力平台测力的实验，研究了散打侧踹腿动作的力量测试方法，开拓了散打侧踹腿动作三维力学测试的新领域；通过对山东省18 名一线优秀散打运动员侧踹腿动作力学特征和运动学特征的研究，揭示了散打侧踹腿动作的运动规律和特征，为诊断、改进和提高侧踹腿动作的技术以及散打教学和训练提供可靠的运动学指标和参数，使散打运动的理论研究进一步系统化、科学化。

This article analyses the application conditions of freezing method, current research status and the problems in thisiold. It, points that it" s necessary to study deep freezing temperature field deeply and discusses the factors which influence the freezing wall such as soil" s nature, moisture content, geological conditions, etc. It introduces the theory, process of freezing method, the distribution of temperature field of freezing method and key thermotics parameters influencing freezing temperature field, it simplifies the deep freezing temperature field model. Through ANSYS, this article founds the deep freezing temperature field model, and simulates the field on vary heat conductivities. It analyzes the results of ANSYS simulation, gains the theoretical value of thermometric holes which is needed by back analysis. Using the simulation temperature and actual value, it gets the equivalent heat conductivity. Then it solves the deep freezing temperature field by the equivalent value, simulates the freezing wall development process and temperature change curve. Against the actual project, the results are favorable.

文中分析了冻结法施工的应用条件，目前对冻结法施工的研究现状和在此领域内存在的问题，指出了对深土冻结温度场进行深入研究分析的必要性；对影响冻结壁形成发展的因素如冻土土性、含水量、地质条件及施工方法等因素进行了论述；对冻结法施工的原理、过程，冻结法温度场的分布情况，影响冻结温度场分布的主要热学参数进行了综述；对深土冻土温度场模型进行了合理的简化，通过ANSYS大型有限元分析软件，建立了深土冻结温度场的模型，对不同导热系数情况下的深土冻结温度场进行了模拟；对ANSYS的模拟结果进行了定性的分析，通过对ANSYS结果的后处理取得了反分析时需要用到的测温孔理论温度值；经过对测温孔模拟温度值和实测温度值的分析，得到了冻结温度场的等效导热系数；使用等效导热系数对深土冻结温度场进行了求解，模拟了冻结壁发展情况和温度场中的温度变化曲线，用所得结果对比工程实际情况，取得了较好的效果。

A mathemetical model of magnetic field in electromagnetic separation of inclusions from molten aluminum under rectangle coil and 50Hz current is presented according to the magnetic vector potential integral equation and the basic theory of magnetohydrodynamics. The magnetic field distribution in the gap of coils is simulated by the model and the actual magnetic field distribution also is measured. The experimental results show that the magnetic field distribution in the gap of coils is uniform and is consistemt with the model simulation. The magnetic field designed accord the simulated result is proved suitable in the practice.

摘 要：根据电磁流体力学的基本理论，利用矢量磁位积分，建立了在采用矩形电磁线圈和工频电源的条件下电磁分离铝熔体中夹杂的电磁场数学模型，模拟计算了电磁感应线圈气隙中的磁场分布状态，同时对制做的感应线圈的实际磁场进行了测量模拟计算磁场的分布状态与实际磁场的测量结果基本一致，都表明在电磁感应线圈气隙中磁场分布均匀，可以进行分离铝熔体中的非金属夹杂。

Secondly, based on the decomposition of gauge potential and the φ-mapping theory,φ-topological field theory of vector and spinor field is established and the Euler class is quantized by nodal indices of vector field.

作为它在量子力学中的应用，分别给出了自旋为零和自旋为1／2粒子的整数及半整数量子化条件，发现它们仅取决于物理系统的内部对称性。

Then for an artificial given solenoidal vector field, the problem how to construct another solenoidal vector field to make its curl equal with the given field was discussed.

作为它的逆问题，对于任意给定的一个标量场和一个无散向量场，求解另一个向量场，使该向量场的散度和旋度分别等于两个给定场的问题，只有在适定的边界条件下才能得到确定的解。

The speed-sensorless vector control and the identification of motor parameters in this paper are the two novel research of vector control.Using asynchronous machine's mathematic model in three-phase static reference frame, this paper has derived it's mathematic model in two-phase static reference frameand in two-phase rotating reference frame, and got the coordinate transformance formulus based on the constant magnetic force and power force, by compared different field orientation methods, we can find that field orientation at rotor field can realize the decoupling of the stator current flux component

无速度传感器矢量控制和电机参数辨识是矢量控制的两个新的研究方向，本文利用异步电机在三相静止坐标系下的数学模型推导出两相静止坐标系、两相同步旋转坐标系下的数学模型，根据磁动势守恒和功率守恒的原则，得出各坐标变换公式；通过不同磁场定向方式的比较发现，异步电机按转子磁场定向时定子电流励磁分量和转矩分量可以比较方便地实现解耦。

Methods Ionization chamber and film dosimetry methods were used to compare the angular dose distribution, point source approximation in the area close to the source,the coincidence of inverse square law of source strength and the scatter and attenuation in water medium,of a cylindrical, miniature 192 Ir source and "the ideal point source".

用电离室法与胶片法实测国产192Ir放射源空间剂量角分布、点源的近似性与源强反平方定律的符合性以及水介质散射与衰减特性，并与点源剂量分布特性比较。

The quality of both red and white wine depends very much on the wine maker talent and on the quality of the grapes.

他的红酒和白酒的品质，根据酿造者的能力和葡萄的质量，各有差异。

No, I don't want to get drunk before my next match.

" "不，我不想让我的下一场比赛前喝醉了。