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The preparation process of equisetum pratense total alkaloid includes the following steps: coarse pulverizing equisetum pratense to obtain rough powder, methyl alcohol percolation to obtain percolate, recovering methyl alcohol, concentrating, degreasing by means of petroleum ether, alkalifying concentrate, CHCl3 extracting, soda lye washing, neutral water washing, recovering CHCl3 to obtain rough alkaloid, adsorption by using silica gel column, eluting, recovering solvent so as to obtain refined EPR.

该发明是通过两个步骤来实现的：第一步，提取草问荆总碱，流程：草问荆粗粉――渗漉液――浓缩液(析胶、石油醚脱脂、氨水碱化)――碱化浓缩液(CHCl3萃取)――CHCl3层――CHCl3液(回收CHCl3)――粗生物碱――吸附硅胶柱――精致EPR。第二步，选择实验试剂、仪器、用小鼠作为实验对象，进行中枢神经系统实验，研究作用及作用机制。

It was shown that suitable ultrasound could enhance reaction rate of lipase catalyzed－esterification in organic solvent.

研究表明适宜的超声辐照可显著提高有机相脂肪酶催化酯化反应的速度。

To decrease the acid value of the product,adding some ethanolamine into the solvent is a good method,but the RI of the product has a slight increase.

为了降低产品的酸值，向溶剂中加入适量的乙醇胺是一个好方法，但是会使产品的折光率略有上升。

However, due to the relatively high solubility of 2 in ethyl acetate, an anti-solvent is required to achieve an optimal yield.

不过，由于较高的溶解度乙酸乙酯2、反溶剂须达到最优产量。

In the two improved processes, ethyl alcohol was the alternative to be used as the solvent.

实验结果表明，这两种改进方法均获得了比传统的共沸脱水工艺更理想的处理效果。

A mixture of solvent is alcohol, ethyl innovativeink that do not include.

溶剂是醇的混分物，油不朱洋不含乙酯。

When using ethyl alcohol as the solvent of the extract, some chromatographic peak shapes became abnormal.

试样以乙醇为溶剂时，会导致某些色谱峰的峰形不好。

And the operational condition was as follows: 100-200 mesh silica gel to be the stationary phase, solvent system of methlene chloride: ethyl acetate: ethanol = 80:35:5 to be the eluent, the flow rate of the eluent to be 2.0 mL/min, the feeding concentration of pristinamycins to be 2.26 g/L, the total loading amount of the silica gel to be 0.81 mg/g and the temperature to be 25℃.

优化后的柱层析操作条件为：选用100～200目的硅胶，二氯甲烷：乙酸乙酯：乙醇=80：35：5为洗脱剂，流速为2.0 mL／min，普那霉素的进样浓度为2.26g／L，硅胶的总负载量约为0.81 mg／g，温度为25℃。

Optimized conditions are as follows:using ethanol solution of sodium ethylate as catalyst, themole ratio of ester to ketone is 1.2:1; the temperature is 90℃; the dosage of solvent is 10mLwith reaction time 5h.

通过正交试验得到了优化实验条件：以乙醇钠的乙醇溶液为催化剂，酯酮摩尔比为1.2：1，反应温度90℃，溶剂用量10mL，反应时间5小时。

The results showed that the proper organic solvent and extractant were sulfonated kerosene and trialkyl phosphine oxide.

结果表明，较合适的溶剂和萃取剂分别为磺化煤油和三烷基氧膦。

Warden: That's a beautiful dog, but don't bring her again.

那是条漂亮的狗，但是不要再把她带来了。

You get up in the morning – no hangover!

你第二天早上起来的时候—不会宿醉！