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In this study, the malondialdehyde content, the glutathione peroxidase activity, and the superoxide dismutase activity in black porgy Sparus macrocephalus were determined after the acute exposure to TBTCl at the concentration of 4 and 20 μg·L-1. Results showed that TBTClinhibited the SOD activity in S.macrocephalus gill slightly , but there was an obvious promotion on the SOD activity in S.macrocephalus liver.

摘 要:观察和比较了黑鲷 Sparus macrocephalus 在不同浓度的氯化三丁基锡(tri-n-butyltin chloride,TBTCl)溶液中暴露后,其鳃和肝脏组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量和谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)活性的变化情况。

Presbycusis might result from the decreased SOD and RCD,and the increased MDA in some extent.

老年性耳聋病人的SOD含量下降,红细胞变形能力降低,而丙二醛水平增高,建议在临床上对老年性耳聋病人应用增加SOD含量,提高红细胞变形能力,减少MDA含量的药物。

It is found that the low dielectric constant inhibits the disproportion of pyrogallic, but it can be settled by increasing the pH or the water content of the reverse microemulsion to obtain a stable generation of the substrate. Then the activity of SOD is determined in such a system and a bell -shaped curve of the activity vs. W〓 is obtained although only subactivity is found. The reasons can be attributed to the low dielectric constant and the attraction of CTA〓.

的介电常数的降低对邻苯三酚的自氧化有抑制作用,因此实验中通过提高CTAB微乳液的表观pH或增大水化度即W〓,最终得到了一定浓度的底物超氧阴离子O〓〓·,保证了SOD活性的测定,并得到了SOD酶活性随含水量变化的&钟形&曲线。

Our research project is focused on the synthesis, structures, spectra characterization and activity research of metal complexes with an amino-Schiff base derived from the salicylaldehyde using as SOD mimics.

本论文的研究主要集中在含有水杨醛氨基乙酸席夫碱的SOD模拟配合物的合成、结构、光谱、热分解动力学,量子化学计算以及SOD活性的测试这几个方面。

Methods Digest recombinant plasmid pU57-SOD with SnaB Ⅰ and Not Ⅰ and insert the obtained SOD gene into expression vector pPIC9k.

方法根据酵母密码子偏好性,合成人Cu,Zn-SOD基因,克隆至真核表达载体pPIC9k中,转化毕赤酵母GS115。

objective:to investigate the influence of soybean isoflavones on the antioxidative effect and improving the ability of study and memory in climacteric rats.methods:forty-eight female sd climacteric rats were randomly divided into 4 groups with one control group and the other low,medium and high dose groups,which were given soybean isoflavones 0 mg/kg.bw,42 mg/kg.bw,83 mg/kg.bw and 249 mg/kg.bw by superalimentation daily for 60 days respectively.the levels of sod,gsh-px and mda in serum were measured,and the ability of study and memory in each group was deterimined by adopting the law of diving platform.results:the content of serum mda in medium and high dose groups was lower than that in control group.the activity of serum gsh-px im medium and high dose groups was significantly increased,so was the activity of serum sod in low,medium and high dose groups.conclusion:soybean isoflavones do have the obvious effect of antioxidation,and improve the ability of study and memory effectively in female sd climacteric rats.

目的:观察大豆异黄酮对更年期雌性sd大鼠抗氧化作用及学习记忆的影响。方法:选用健康更年期雌性sd大鼠,随机分成空白对照组和低、中、高剂量4组。空白对照组给予蒸馏水,实验组每日分别灌服42、83、249 mg/kg剂量的大豆异黄酮,连续60d。第60d取血测定血清过氧化脂质丙二醛含量、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力,用跳台实验方法测定大鼠学习记忆能力。结果:与空白对照组比较,中、高剂量组大鼠血清mda含量显著降低,低、中、高剂量组血清sod活力明显升高,中、高剂量组血清gsh-px活力明显升高。结论:大豆异黄酮具有明显的抗氧化作用,并有效提高更年期雌性sd大鼠的学习记忆能力。

The protection of AF-1 was observed with the measurement of transudatory rate of LDH ,the breaking rate of DNA and contents of LPO and SOD,Results:The intotoxincation of glutamate was decreased by AF-1 which reduced the transudatory rate of LDH,the breaking rate of DNA and the content of LPO,and increased the content of SOD in culture significantly.

通过测定乳酸脱氢酶漏出率、DNA断裂率、超氧化物歧化酶和脂质过氧化物,观察AF-1药物的保护作用。结果:高浓度的谷氨酸可增加培养神经细胞的LDH释放率和DNA断裂率,并诱导自由基产生。AF-1明显降低谷氨酸的神经细胞毒性,使LDH释放率及DNA断裂率明显降低,LPO含量明显下降,SOD含量明显升高。

The results showed that structure of root tissues became disorder and appeared void with increase of the N and P concentration; The content of N, P were increased gradually with the N and P concentration increased; activity of POD, SOD and CAT of Vallisneria spirolis were enhanced gradually with increasing the N and P concentration.

研究发现,随营养程度的增加,苦草根的组织结构紊乱,出现空洞;根叶中氮、磷含量都是随营养程度的增加而增高;苦草叶组织的POD、SOD、CAT活性均随营养程度的增加而上升;随培养时间的延长苦草叶组织的POD和CAT活性呈先上升后下降的趋势,SOD活性下降。

The results showed that in the growth period of R. glutinosa, soil ferulic acid, vanillic acid, and vanillin contents had a persistent increase, while β-hydroxybenzoic acid content increased first but decreased then. The addition of ferulic acid (8 μg·ml-1), vanillic acid (08 μg·ml-1), vanillin (12 μg·ml-1), and β-hydroxybenzoic acid (30 μg·ml-1) in water culture had stronger inhibitory effects on R. glutinosa root length, root mass, fresh plant mass, and plant height. Treating with test phenolic acids except β-hydroxybenzoic acid increased leaf chlorophyll content significantly, compared with the control. After treated with test phenolic acids, plant SOD and POD activities decreased after an initial increase, and plant malondialdehyde content increased. In the treatment of ferulic acid, root SOD and POD activities were the lowest, and roots were rotted and dead.

结果表明:阿魏酸、香草酸和香草醛含量在地黄生长期表现为持续增加,而对羟基苯甲酸含量先升高后降低;外加阿魏酸(8 μg·ml-1)、香草酸(0.8 μg·ml-1)、香草醛(1.2 μg·ml-1)和对羟基苯甲酸(3.0 μg·ml-1)对水培地黄根长、根质量、全株鲜质量和株高有较强的抑制作用;酚酸处理使地黄叶片叶绿素含量降低,除对羟基苯甲酸外差异性极显著;经过酚酸处理的地黄幼苗SOD和POD活性为先升高后下降,丙二醛含量升高,其中阿魏酸的处理使根中酶活性降至最低,根部腐烂死亡。

Methods: The subjects were treatmented by the device two weeks30 minute every day,.while the activity of SOD and content of MDA in veinous blood on an empty stomach wore determined before and after treatment with magnetic therapy.

用此机给受试者每日磁疗一次,每次30分钟,两周为一疗程,于磁疗开始前及一疗程后取空腹静脉血测定血清SOD活力及MDA含量。结果:磁疗后血清SOD活力显著升高(P.01),MDA含量明显降低(P.01)。

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