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Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中，经39 000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Microcells were produced by centrifugation of micronucleated A9 (neol2) cells in Percoll density gradient containing 20mg/L Cytochalasin B at 39 000g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 pm and 5 pm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20mg/L细胞松弛B的Percoll密度梯度介质中，经39000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

17 Chapter 5 Operating System 5.1.1 Resource allocation and related functions When a resource is sequentially shareable, the system can deallocate a resource when the program makes an explicit request for de-allocation.

当资源可以串行共享时，当资源可以串行共享时，系统可以在程序明确地提出释放分配的请求后，对资源进行释放。

1 Resource allocation and related functions When a resource is sequentially shareable, the system can deallocate a resource when the program makes an explicit request for de-allocation.

当资源可以串行共享时，系统可以在程序明确地提出释放分配当资源可以串行共享时，的请求后，对资源进行释放。

15 Chapter 5 Operating System 5.1.1 Resource allocation and related functions When a resource is sequentially shareable, the system can deallocate a resource when the program makes an explicit request for de-allocation.

当资源可以串行共享时，系统可以在程序明确地提出释放分配当资源可以串行共享时，的请求后，对资源进行释放。

Based on the criterion that the volume of estimated parameter's joint confidence region should be minimum, which is a sequential experimental design norm for optimal parameter estimation proposed by Box and Lucas, the observations for the adsorption of n -decane on 5 A molecular sieves were determined sequentially, and it is found that the observations should b.

根据Box和Lucas所提出的使待估参数联合置信域的容积趋于最小的参数估算的序贯实验设计准则，推导得出了正葵烷吸附等温线实验观测点的安排原则，发现对于一元线性模型，实验观测点应安排在可操作区的两端。

Shiv performs the entire task sequentially and these tasks are very determent.

希夫整个任务执行顺序，这些任务十分determent。

The results felicitously explain that the number of introns distributed in vertebrates , Diptera and nematodes are declining sequentially .

该结果合理地解释了内含子在脊椎动物，线虫及昆虫中数量的分布呈下降趋势。

The article anatomizes the systems limits based on the research to the running effect of it, clarifying framework problems and operating difficulties in theimplementing process. It also carried out sensible exploration mainly from the point of the weakness of international example, disjointedness in financial management and business development, the limit of financial staff quality, the difference of performance index, the vacancy of financial management main body etc. The conclusion is that it must be improved for enterprises to win value-added service from financial information support in competition, and be able to supervise the status of implementing cooperate strategy and progress of operation goal realization, sequentially, improve core competitiveness.

本文在针对构建财务集成系统运做研究的基础上，对局限性进行了深入的剖析，理清目前财务集成在实施操作过程中的框架性问题和操作运行难点，主要从国际母本缺陷、财务管理与业务发展脱节、财务人员素质局限性、指标体系的差异、财务监督主体缺位等角度对其形成原因进行了理性探讨，认为必须进一步加以改进才能使企业在竞争中获得来自财务信息支持的增值服务，能够全程监控企业经营战略的实施情况和经营目标的实现状态，进而提高企业集团的核心竞争力。

The application of our products in the processing of core-wrapped electric wire, deoxidizer for steel-making, Calcium, Chromium, Strontium, Ferroniobium, Ferromolybdenum, Ferrotitanium and Ferrovanadium will significantly improve the recovery rate, reduce the impurity of the products and enhance the product quality, which sequentially brings more economic benefit to your company.

在生产包芯线、炼钢脱氧剂、金属钙、金属铬、金属锶、铌铁、钼铁、钛铁和钒铁等中使我公司生产的铝粉和铝粒，可提高回收率，降低产品杂质，提高产品品位，从而获得更好的经济效益。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。