查询词典 sequencing

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

It can be concluded by 16S rDNA sequences that moderately halophilic bacteria are novel species of genus Marinococcus and genus Lentibacillus. Further study for proving this novel observation is still in progress. At the same time, halophilic archaea strains, broken in distilled water and haloalkaliphilic archaea strains, grown in medium of pH9.5 were isolated from these samples and sequencing of the 16S rDNA sequences are being conducted taking other polyphasic taxonomy identification.

中度嗜盐菌通过对16SrDNA序列得测定，初步推断可能有Marinococcus属和Lentibacillus属的新种，后续鉴定工作还在进行中；同时，分离到到能在蒸馏水中破壁的嗜盐古菌菌株，另外分离到在pH9.5培养基中生长的嗜盐嗜碱古菌菌株，正在对其进行16S rDNA序列的测定和其它多项分类鉴定工作。

The specific primer was further designed. Using the ray floret as material, after RT-PCR, T-A clone and sequencing, a 732 bp sequence was obtained.

进而设计特异引物，以非洲菊舌状花为材料，通过RT-PCR扩增并经T-A克隆后测序，获得一条长度为732 bp的序列。

In our former study, we cataloged genes expressed in human brain (about 10,000 gnes), and sequenced the orthologous sequence of these genes in chimpazee. In this study, we selected 16 of these positive-selection genes and then using PCR and Sequencing technique we acquired orthologous sequence in another two representative primates: white-brown ape and macaca, and then took further evolutionary analysis.

本文从目前已知在人的大脑中表达的基因中，选出16 个在人和黑猩猩的比较中明显受到正向选择的基因，利用PCR扩增技术和基因序列测定技术，获得了另外两个灵长类代表动物小猿、猕猴的直系同源序列，并进行了进化分析。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Organization: College of Environmental Science and Engineering, Guilin University of Technology;State Key Laboratory of Environmental Engineering and Protected Assessment in GuangxiAbstract: Anaerobic granular sludge was rapidly cultivated in a sequencing batch reactor using glucose and steaming process waste water as carbon source, and anaerobic granular sludge in well-operated upflow anaerobic sludge blanket reactor and aerobic activated sludge as inoculation sludge.

摘 要：在序列间歇式活性污泥法(sequencing batch reactor， SBR)反应器中，先后以葡萄糖和竹品蒸煮废水为碳源，用运行良好的上流式厌氧污泥床（upflow anaerobic sludge blanket， UASB）反应器中厌氧颗粒污泥和城市污水处理厂好氧活性污泥为接种泥，在充氧条件下快速培养好氧颗粒污泥。

On the basis of the obtained results, a simple two-pass DNA sequencing scheme is suggested.

以获得的结果为基础，提出了一个简单的双通道DNA测序方案。

The technique is so simple,rapid,low cost and easy-to-use that it is bound to provide powerful technique for cotton genome sequencing.6.Researching on application of cotton FISH systems.There are two-pair large signals on the mitosis metaphase chromosomes and pachytene chromosomes of G.arboretum Shixiya 1 hybridized with 45S rDNA probe.

该技术方便快捷，成功率高，成本低，容易被掌握和快速推广，必将为深入进行棉花基因组研究乃至棉花基因组测序的最终完成提供强有力的技术支持。6。

The Small-tailed Han sheep(48), Ningxia Tan sheep(121), Tan SH (23), Poll Dorset(48), Suffolk(24) were used to study the single nucleotide polymorphisms of H-FABP gene by PCR-SSCP and DNA sequencing. Five nucleotide variations were found, of which G981A, A1014C, and T1019C resulted in amino acid changes of R327H, E338A, and S340P, respectively.

以小尾寒羊(48只)、宁夏滩羊(121只)、滩寒杂交羊F1(23只)、无角陶塞特(48只)、萨福克(24只)5个绵羊群体为实验材料，利用PCR-SSCP和DNA测序技术对心脏型脂肪酸结合蛋白基因(GenBank登录号： AY157617)外显子2和内含子2部分序列进行单核苷酸多态性检测及遗传多态性分析。

And to identify by nucleotide sequencing and restriction endonuclease digestion.(2) The recombinant plasmid was transformed into Bacillus coli and to shake culture.

将pET28a-hnRNPA2/B1重组质粒转化入大肠杆菌BL21振荡培养，用IPTG诱导表达融合蛋白并且优化条件，以SDS-PAGE分析融合蛋白的存在及可溶性。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。