查询词典 sequencing

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Fifty-nine insect species have been included in genome sequencing projects, whole-genome sequences of six insect species, i.e., Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera, Aedes aegypti and Tribolium castaneum, have been reported.

目前有59种昆虫已经列入基因组测序计划，其中6种昆虫（黑腹果蝇Drosophila melanogaster、冈比亚按蚊Anopheles gambiae、家蚕Bombyx mori、意大利蜜蜂Apis mellifera、埃及伊蚊Aedes aegypti和赤拟谷盗Tribolium castaneum）的全基因组序列已经报道。

One positive clone was obtained from the libraries with the tributyrin flat and ELISA sieve method. Analyzed the sequencing result, the positive clone include the Open Read Frame of low-temperature alkaline lipase gene.

序列测定分析表明，此重组质粒包含有长度为此95lbp的低温脂肪酶基因的完整的开放读码框架和上游调控序列。

Results The mecA gene and tst gene were detected,and were made the gene sequencing successfully.

结果 成功的对金黄葡萄球菌mecA基因和tst基因进行了检测，并进行基因测序。

The best bets for ultrarapid, low-cost sequencing are single-molecule approaches.

对于超高速、低花费的测序最好的办法是单分子的方式。

The effect of excess sludge reduction by uncoupler of 2,4-dechlorophenol was studied using Sequencing Batch Reactor.

采用序批式活性污泥反应器试验研究了解偶联剂2，4-二氯酚的污泥减量化效果。

This research was on sequencing batch reactor activated sludge system, using 2,6-dichlorophenol as uncoupler with the concentration of 20,using Cu~(2+) with the concentration of 1,adding into the SBR activated sludge system.

采用间歇式活性污泥培养装置，以2，6-二氯苯酚作为解偶联剂，浓度为20 mg/L，Cu2+浓度为1 mg/L，加入到间歇式活性污泥反应器中。

The unfused human interferon aD expressed by pBV867 was confirmed by SDS-PAGE,activity assay and N terminal sequencing.Results suggested that E.Coli is able to process the signal sequence of human interferon αD.

发现&翻译联结&方式起始的基因表达水平比一般ATG起始方式高约10倍；证明大肠杆菌能够识别并加工人αD型干扰素信号多肽。

To get more factors associated with erythropoiesis in K562 cells, modified differential display reverse transcription polymerase chain reaction method was applied to identify genes with differential expression in uninduced and hemin-induced K562 cells. Remarkable differential ESTs were firstly selected for cloning, sequencing and analyzed with bioinformatics methods.

为了进一步获得分化过程中相关基因表达的信息，我们应用改良的差异显示RT-PCR技术，分析K562细胞在Hemin诱导的红系分化前后的基因表达变化，获得红系分化相关基因的线索，进而确定在红系分化中起重要作用的基因，并研究它们的作用机制。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

When this condition occurs, inbound replication with the source partner is stopped on the destination domain controller and event ID 2042 is logged in the Directory Services event log.

计算机密码学是研究计算机信息加密、解密及其变换的科学，是数学和计算机的交义学科，也是一门新兴的学科。

Instructions: click on the thumbnails to see a larger image, then use the left-right arrow keys to scroll through the slideshow.

使用说明：滑鼠点在小图上即可放大观赏。开启后键盘左右键可用来换照片。