查询词典 sequencing

We expect that the transgenictomato plant become a bio-reactor and develop a new way to product wild-typep53 protein.In this study,the coding sequencing of human wild-type p53tumor suppressor was ligated with the promoter and terminator of phaseolin,which is expressed specially in plant seeds.

本实验试图将人野生型p53肿瘤抑制基因引入番茄植株，通过采收番茄果实很方便的获得p53蛋白，使转基因番茄成为p53蛋白的生物发生器，开辟一条生产p53蛋白的新途径。

The isolated strains were subjected to phenotypical study,salt-aggregation test and rDNA ITS sequencing.

对获取的活性菌株进行形态特征研究、盐需求测试、核糖体rDNA ITS区序列分析，并将所测得序列在NCBI数据库中进行相似性搜索，选取其中相似性较高的序列以邻接法构建系统进化树，将该菌株分类鉴定到种。

Genome and EST sequencing projects generate a wealth of sequence information for agronomically and phylogenetically important plant species of which the majority is difficult to subject to functional genomics.

农艺上和系统发生上重要的植物物种的基因组和EST测序计划产生了大量的序列信息，其中大部分难以服从功能基因组学。

Patients with clinical virologic breakthrough during NA therapy and 4 negative control patients without virologic breakthrough were selected for the study of quasispecies dynamics. All the 70 serum samples from these 10 patients were analyzed with the method of'PCR-cloning-sequencing'. Totally over 2,100 clones were sequenced. The target sequences obtained were aligned, compared and analyzed to study the mechanism of NA drug resistance and to understand the clinical course of NA therapy from a viral populational genetic point of view.

我们进一步采用国际认可的&金标准&&PCR-克隆-测序-序列分析&的方法，对6例发生临床病毒学突破的患者以及4例未发生临床病毒学突破的患者的全程血清样本进行了准种检测，共测序2100余个，以系统深入地对LAM和ETV治疗过程中HBV种群的演变及其临床意义进行探讨。

Genetic studies have been performed on the hereditary diseases of disseminated superficial actinic porokeratosis, familial progressive hyperpigmentation and triphalangeal thumb-polysyndactyly syndrome by linkage analysis and sequencing.

本研究通过连锁分析和直接测序的方法，分别对播散性浅表性光化性汗孔角化症、家族性进行性色素过度沉着症和拇指三指节多指并指多掌骨畸形这三种疾病进行了遗传学分析。

Genetic studies have been performed on the hereditary diseases of disseminated superficial actinic porokeratosis, familial progressive hyperpigmentation and triphalangeal thumb-polysyndactyly syndrome by linkage analysis and sequencing.

中文摘要本研究通过连锁分析和直接测序的方法，分别对播散性浅表性光化性汗孔角化症、家族性进行性色素过度沉着症和拇指三指节多指并指多掌骨畸形这三种疾病进行了遗传学分析。

In attempt to elucidate the molecular mechanism of cold tolerance of wintersweet, which blooms in the deep winter,a COR413 protein gene, designated as Cpcor413pm1, was obtained by sequencing the randomly selected clones, on the basis of Chimonanthus praecox flower cDNA library construction and its ESTs analysis.

为研究蜡梅冬季开花过程的抗寒分子机理，在构建蜡梅花cDNA文库及EST分析的基础上，通过随机克隆测序，克隆了1个蜡梅COR413蛋白的cDNA基因，命名为Cpcor413pm1。

Methods A strain of Propionibacterium acnes from rumen fluid of health cow with permanent rumen fistula under sterile condition was isolated by segregation procedure of anaerobic bacterium and specific medium SLB, and identified by extraction of the genome DNA of isolation bacterium, clone of the 16S rRNA gene, and sequencing of the strain.

无菌采取装有瘤胃瘘奶牛的瘤胃液，按照厌氧菌分离步骤，通过丙酸生成菌株的特异性培养基SLB进行筛选，提取分离菌的基因组DNA，克隆其16S rRNA基因，进行序列测定，分离出一株痤疮丙酸杆菌。

Three genes specified expressed in hybrids and one gene over-expressed in hybrid were found. By cloning, sequencing and BLAST analysis, one specific expression cDNA band was cytosolic isocitrate dehydrogenase gene, the other two were new genes which function were unknown, and the over-expressed cDNA band was proteosome subunit p112 gene.

发现了 3 个杂种鸡特异表达的基因和 1 个杂种鸡表达增强的基因，进一步克隆、测序和比对分析初步表明：杂种特异表达的 cDNA 片断分别为鸡细胞质异柠檬酸脱氢酶基因和 2 个未知功能的新基因；杂种表达增强的 cDNA 片断为鸡蛋白酶体亚基 p112 基因。

Methods: HPV 16/18 infection was detected by nested-polymerase chain reaction, the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas.

本研究利用巢叠式聚合脢锁反应分析自129例罹患翳状赘肉之翳状赘肉组织及20例正常无翳状赘肉的结膜组织检体中，是否有人类乳突病毒16及18型的感染，并利用直接定序的方式检测p53基因是否发生突变，此外亦以免疫组织化学染色法侦测p53蛋白与人类乳突病毒E6蛋白。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。