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By using the traditional taxonomic methods, electronic microscope examination, 18S rDNA, ITS sequences and phylogenetic analysis, an endophytic fungus HCCB1621 isolated from the cephalotaxus sinensi was identified as Trichoderma viride Pers.ex Fr. Its anti-tumor activities were studied as well.

利用传统分类学方法、电子显微镜观察、18S rDNA和ITS序列的测定以及系统发育分析对一株分离自粗榧抗肿瘤活性内生真菌HCCB1621进行分类鉴定，并对其产物抗肿瘤活性进行了初步研究。

The present study eatablished genechip technique for Ceratitis capitata, C. rosa and C. cosyra, in which the mitochondrial cytochrome oxidase Ⅰ gene was used as molecular marker, and the DNA sequences of fruit flies (Diptera: Tephritidae) were used as target sequence. The genechip detection system for fruit flies was composed of detecting probes and quality-control probes for C.

本研究选择线粒体DNA细胞色素氧化酶Ⅰ基因为分子标记基因，以双翅目实蝇科昆虫DNA序列为目标，建立了我国进境植物检疫害虫地中海实蝇Ceratitis capitata、芒果小条实蝇C.cosyra和纳塔尔小条实蝇C.rosa等生物芯片检测方法。

In order to develop an accurate and efficient method for the identification of tiger DNA,mtDNA Ctyb sequences of 5 tiger subspecies and 6 other feline species and 6 cervid species were downloaded from Genbank,and compared by Wdnasis(V2.5) software.

为了建立对虎DNA进行特异性检测的有效、精确的方法，本研究中，从Genbank下载虎及其他6种猫科动物和6种鹿科动物的mtDNA细胞色素b基因序列，并用Wdnasis(V2.5)软件进行了种间序列比较。

According to the alignment analysis of full length sequences of Cytb gene from closely relate species of Cervus , one pair of allele-specific diagnostic PCR primers was designed. Factors such as annealing temperature, dosage of polymerase, times of cycles and dosage of template DNA that influence the PCR results were also investigated.

根据鹿科鹿属种动物Cytb全序列比对分析，设计1对能准确鉴别正品鹿茸和其他近源种鹿茸的引物，在此基础上建立鹿茸药材位点特异性PCR鉴别方法，并对影响PCR结果的主要因素退火温度、 Taq 用量、循环次数以及模板用量和重复性等进行方法学考察和优化。

Partial sequences of chalcone synthase gene exon 2 were amplified by PCR method from genomic DNA of 10 species of the basal eudicots. After cloning and sequencing, 26 seqences were obtained.

用PCR方法从目前极少被报道的真双子叶基部类群10种植物的总DNA中扩增出CHS基因外显子2的部分序列进行分析，经克隆后测序，共得到26个不同的片段。

Moreover, we we sequenced the entire mitochondrial DNA control region from 17 charadriiform species, and found that the base composition and distribution of the variable nucleotide sites within the control region were similar to other avian species, but a series of short tandem repeated motifs in the 3' end of domain III made A nucleotides and length increased significantly. Phylogenetic analysis showed that these sequences could not well resolve the relationship among genus in Charadriiformes, but within genera maybe has some valuable phylogenetic information.

此外，本研究测定了鸻形目17种鸟类线粒体调控区序列，发现在碱基组成和进化速率上与其它鸟类相似，但其3'末端的串联重复序列导致domain III长度且A含量显著增加；利用调控区序列未能较好的解决鸻形目属以上水平的分类，但对于解决属内物种间的进化关系具有一定的参考价值。

It is considered that influenced by the strike-slip movement of Tanlu Faults and "episodic" chasmic evolution, buried hill draping structures with different sequences are created from the north to south in Kandong-Zhuanghai buried hill draping structure.

认为受郯庐断裂带走滑运动和渤海湾盆地第三纪&幕式&裂陷演化的影响，垦东－桩海潜山披覆构造带自北向南形成不同位序的潜山披覆构造。

The sequences shared high homology with hsp70a,with identity 96% to Chlamydomonas reinhardtii,94% to Petunia,93% to Pisumsativum,92% to tomato,92% to human,90% to Drosophila and 89% to yeast respectively.

推导的氨基酸序列与与下列物种的hsp70a比较：衣藻为96%，矮牵牛花为94%，Pisumsativum为86%，番茄为86%，人为85%，果蝇和酵母分别为84%和83%。

By searching for genetic sequences similar to regA in the burgeoning databases of genes that now exist, they found one in a unicellular alga called Chlamydomonas reinhardtii.

他们在现有的尚处于萌芽阶段的基因数据库中搜索与regA的基因序列相似的基因，并找到了一种属于单细胞藻类 Chlamydomonas reinhardtii 的基因。

By searching for genetic sequences similar to regA in the burgeoning databases of genes that now exist, they found one in a unicellular alga called Chlamydomonas reinhardtii.

他们在现有的尚处于萌芽阶段的基因数据库中搜索与regA的基因序列相似的基因，并找到了一种属于单细胞藻类

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。