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The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.

根据氨基酸自动分析仪测定的氨基酸组成和精确分子质量计算出NSPC分子总共有331个氨基酸残基，分别是由40个Asp、22个Thr、18个Ser、22个Glu、1个Gly、31个Ala、27个Cys、17个Val、13个Ile、22个Leu、5个Tyr、7个Phe、7个Lys、24个His、51个Arg、14个Pro、1个Met和9个Trp组成。胰蛋白水解酶分别酶解NSPC-1和NSPC-2后，分别采用MALDI-TOF-MS分析，其肽指纹谱图显示胰蛋白酶可将NSPC-1和NSPC-2水解成大小不等的多条肽段，质量数在800以上的NSPC-2有11段，即水解的肽质量分别为899.54、1454.77、1544.9、1683.87、1803.93、1811.91、1987.92、2003.90、2536.26、2915.42和3492.72。

After circularization by self-ligation, inverse polymerase chain reaction is used to amplify the integration sequences of the human high metastatic potential hepatocellular carcinoma cell line MHCC97-H genome.

对酶切片断进行自身环化后，运用反向聚合酶链反应对人高转移潜能肝癌细胞株MHCC97-H基因组中整合序列进行扩增。

The amplified full-length plasmid PCR products were transformed into competent E. coli and they were able to circularize due to the terminal homologous sequences.

转化到细菌中的全长质粒DNA PCR产物可利用其末端同源序列发生同源重组而环化。

These coronaviruses are not closely related in terms of gene sequences to IBV. Andas a result, they are clearly not simply isolates of IBV, but represent new coronavirusspecies. The phenomenon of IBV is not limited to replicating in a single host species, whichwas highlighted by the spread of SARS-coronavirus from mammals, like civet cat andbat.

IBV这种从感染单一宿主延伸到更多的其他动物的现象，让人联想到2002年爆发的"严重急性呼吸系统综合症"(Severe acute respiratory syndrome，SARS)，它的病原是一种新的冠状病毒(严重急性呼吸系统综合症冠状病毒，SARS-CoV)，而在多种动物体内分离到的SARS-CoV样冠状病毒说明SARS-CoV极有可能最初起源于动物。

The structure theorem is obtained by defining "ternary ordered partition method" which is used to choose the test-sets of the first $k$ tests, and by defining two operations and two relations on feedback sequences; The isomorphism classification theorem is strictly proved by virtue of the structure theorem, thus the complicated problem of discussing $3^k$ possible search domains is reduced to the relatively easy problem on $k+1$ representative search domains; By the techniques of "super-coin construction method" and "image-union method", the search problem on $k+1$ representative search domains is reduced to the simple verification of several conditions on finite space of small cardinality.

通过引入用于选取前$k$次试验的试验集的"三元有序剖分法"以及关于反馈序列的两种运算和两种关系，得到了结构定理；基于结构定理严格地证明了同构类定理，从而将必须针对$3^k$个搜索域的复杂问题转变为讨论$k+1$个具代表性的搜索域的相对简单的问题；通过"构造超币法"和"并像法"等技术手段，将关于$k+1$个具代表性的搜索域的搜索问题转化为简单的关于势非常小的有限空间的几个条件的验证问题。

There are two types of sequences in these cycles; clastic rock sequence and salt rock sequence.

层序类型有两种，既陆缘碎屑岩层序和盐岩层序。

The results of experiments on a great deal of image sequences of closely packed particles indicate that, for closely packed aggregates particles, quite reasonable estimates of number of particles or average size often arise from the method based on the edge density.

通过对大量颗粒图像进行的实验，特别是岩石骨料图像序列的实验结果显示，对于紧凑型的石料颗粒，采用基于边缘密度的方法检测颗粒数量或者平均尺寸常常可以收到相当好的效果。

The results of phylogenetic analysis indicated that various L-GalDH sequences were clustered into three groups.

系统进化分析结果表明不同来源的L-GalDH基因聚为3类。

Microsatellites are composed of simple tandem repeats of nucleotides primarily in non-coding gene sequences. The number of repeats is inherited as a codominant trait.

摘 要：微卫星主要是指由非编码基因序列核苷酸的简单串联重复，其重复的数目呈共显性遗传。

After analyzing glutinous rice waxy gene sequence reported by previous studies, two pairs of dominant and codominant STS molecular markers based PCR were designed for distinguishing glutinous rice, according to the distinctions between glutinous rice wx gene and nonglutinous rice Wx gene. Moreover, the whole sequences of the two Wx genes of nonglutinous rice reported by others were also aligned, and another two pairs of dominant STS molecular markers were designed to discriminate Wxa from Wxb based on the special site between the two genotypes. Then, corresponding rice genotypes were identified by the established new markers.

通过对前人公布的糯性水稻蜡质基因全序列测定结果的分析，根据糯性水稻与非糯性水稻蜡质基因序列位点的差异，设计了两个基于PCR扩增反应的可特异识别糯性水稻的显性和共显性STS分子标记；同时在前人公布的非糯性水稻蜡质基因的不同类型的全序列比对分析的基础上，选取了两种基因型特定的差异位点，设计了两个基于PCR扩增反应用于特异识别这两种Wx基因的显性STS分子标记，并用新建立的标记对相应的水稻基因型进行了检测。

I hope that this success is just the first in a long series.

我希望这只是成功的开始。

Three dimensional stresses on the interface of tire and soil, tire deflection and soil sinkage are measured.

测试轮胎——沙土界面上的三向应力、轮胎变形和沙土沉陷；建立考虑剪切应力对沙土承载特性产生影响的车轮牵引性能预测模型；分析车轮作用下土壤的纵向稳定性。