查询词典 sequence

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Based on the sedimentary combination, lithology and sequence characteristic, two classes of sequence structures were generalized as structure entireness and structure lackness.

并根据剖面纵向沉积组合、岩性特征及层序特征，概括出结构完全型和结构缺失型两类层序结构。

The most common approach is to consider the problem of machine equivalence which defines two synchronous design specifications functionally equivalent if, clock by clock, they produce exactly the same sequence of output signals for any valid sequence of input signals.

最常用的方法是考虑的问题，机等价定义两个同步设计规格等同功能，如时钟的时钟，它们产生完全相同的序列输出信号的任何有效的序列输入信号。

Chapter 3, in the above system, gives out the definitions of topological new transitivity, topological transitivity, topological strong transitivity and topological conjugate of a sequence of maps, studies basic properties of the above topological conjugate, and obtains some main results, proves topological new transitivity and topological transitivity are equivalent u-under a compact metric space, a sequence of full maps and interchangeable with each other, and that some conclusions associated with topological conjugate.

第三章，在上述系统中给出了一列映射的拓扑新传递、拓扑传递、拓扑强传递和拓扑共轭的定义；研究了一列映射的拓扑共轭的基本性质，得到了一些主要结果；证明了在底空间是紧致度量空间、一列映射是满映射并且两两可交换的条件下拓扑新传递和拓扑传递是等价的；还证明了几个与拓扑共轭相关的结论。

On the basis of analyzing the characteristic of the basic immune algorithm and utilizing the ergodic property of the chaotic sequence, an adaptive immune evolutionary algorithm combined with chaotic sequence is proposed and used at the training of the neural networks.

在分析基本免疫算法优化性能的基础上，利用混沌序列的遍历性，给出了一种嵌入混沌序列的自适应免疫进化算法，并将其应用于神经网络的训练中。

The common denominator of both is 20. 1 applied the Fibonacci sequence to the number 20 and carried the sequence out to 26 places.

两者的共同数字是20.1应用在数目为20的斐波纳契序列上，把序列携带到26处空间。

Niu_jiantao的正确 The Fibonacci sequence 1,1,2,3,5,8,13,21……starts with two 1s,and each term afterwards is the sum of its two predecessors,which one of the ten digits is the last to appear in the units position of a number in the Fibonacci sequence?

Fibonacci数列1，1，2，3，5，8，13，21……以两个1开头，此后每一个数都是前两个数的和。那么在Fibonacci数列中，10个阿拉伯数字中的那一个是在个位数上最后出现的？

It is proved that a generalized Ishikawa type iteration sequence convergences to the unique common fixed point of its more generalized quasi-contractive mapping sequence respect to p.

证明了广义Ish ikawa型迭代序列收敛于关于p的更广义拟压缩映射序列的唯一公共不动点。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。