查询词典 sequence of differences

Phylogenetic analysis based on two IGS-1 sequences indicated there were significant differences between the IGS-1 sequence of Armillaria luteo-virens and that of other species of Armillaria.

本研究首次基于分子手段对我国青藏高原的黄绿蜜环菌种进行了分离培养、分子鉴定和系统发育分析，为黄绿蜜环菌的科学分类提供了分子依据。

There are no distinct differences between ancestral character state and descendant character state in some characters. 4. Only one outgroup can't give enough information to tell what the ancestor looks like. It make us not to understand how a lot of charactors evolved. 5. The matsutake group is polyphyletic. 6. The character coding is not precise enough. 7. Some characters are not homology. The result of molecular systematic study on the matstuake group shows the group is not monophyletic. The matsutake group is more closed to Tricholoma than Armillaria. ITS sequence of Catathelasma ventricosum is nearly identical to that of the matsutake group, although apparently they are very different from each other in morphological characters.

对松口蘑群通过形态学特征进行系统发育关系分析造成很大的干扰的原因有：松口蘑的种类大多形态特征比较简单，可提供进行分析的特征不多；某些性状在一些状态之间的反复转化，造成分析上的误差；在进化过程中某些性状无明显的祖征和衍征，在分析过程中很难确定进化极性；选择外群时应该用一个特定类群进行整体理解后，确定各个性状的极性，不要只用单种作为外群；松口蘑群是一个多系群；进行比较的性状不一定是同源的；数量性状编码过于粗放，反映不出真实的情况。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

为了从分子水平阐明十字花科植物间的亲缘进化关系，给植物种质资源的创建提供理论依据，试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物，运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列，经比较分析，结果表明：这些同源序列的相似性达80%以上，所推导的氨基酸序列相似性达70%以上，且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%，属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%；由氨基酸序列构建的分子系统树可知，在亲缘进化关系上芸薹属与萝卜属较近，其他依次为蔊菜属、拟南芥属、荠菜属，而与诸葛菜属最远。

No fragment length nor nucleotide sequence differences were detected for 2 chloroplast intergenic spacers between Chilanshan and Alishan populations in both Chamaecyparis species.

结果发现红桧与台湾扁柏於栖兰山与阿里山两族群间，在两个叶绿体DNA之基因间区间序列其限制酶片段长度及核苷酸序列均没有差异。

Methods According to differences in internal transcribed space sequences of Penicillium genus and specific IAO sequence, we designed species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum and established the corresponding PCR systems.

根据 GeneBank 中青霉属不同种和相近属种的 ITS 序列差异和灰黄青霉特有的 IAO 序列，设计了污染区优势种灰黄青霉的特异性引物 AS1/RS4 和 IAO1/IAO2，建立相应的特异探针检测体系。

The protozoa were isolated from field animal blood, vectors, pathogenic tissue and secretions. Based on differences of the pathogen morphology, vector species, experimental infected animals, single parasite clone and parasitic tissues, protozoiasis pathogens from different areas and animals in China were isolated and the vectors and transmission mode were also determined by vector transmission trial. Sequence analysis of 18S rRNA gene was used to study the phylogeny of partial isolated protozoa.

自野外采集动物血液、传播媒介、相关组织、排泄物等，根据对病原形态鉴定、传播媒介鉴定、实验性感染易感动物、单一虫株克隆、不同致病部位等方法，对我国不同地区、不同家畜的原虫病病原进行分离，并通过媒介传播试验确定传播媒介的种类与传播方式；采用18S rRNA基因测序方法，对本实验室已分离的部分原虫进行进化关系研究。

In this thesis, we compare the differences and similarities of theme and rheme in English and Chinese, study their influence on sentence structure, sentence sequence, context coherence and illustrate how to use them to translate faithfully, expressively and closely.

本文从英、汉两种语言主、述位特点和主、述位推进模式出发，探讨其对句子结构、排列顺序及语篇衔接连贯的影响，并进一步阐释如何利用他们之间的异同进行翻译，真正做到准确、地道地传达原文的风采。

The purpose of paper is to describe the petrology character of outcrop sandstones and core sandstones and judge their roke type, compare the similarities and differences of autogenetic mineral、diagenesis types、intergrowth sequence、diagenetic facies、diagenetic phase of outcrop sandstones and core sandstones, provide academic precondition condition for studying better reservoir in studied region.

本次论文的研究目的是描述露头砂岩和岩芯砂岩岩石学特征并判断其岩石类型，比较露头砂岩与岩芯砂岩在自生矿物、成岩作用类型、共生序列、成岩相、成岩阶段以及成岩流体演化方面的异同，为研究区有利储层的研究提供理论前提条件。

BACKGROUND: Recognition of relevant sequence deviations can be valuable for elucidating functional differences between protein subfamilies.

背景：识别相关的序列偏离对于阐明蛋白质亚家族间功能上的差异是有价值的。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。