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It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能,本研究选择了MAP的主要保护性抗原Hsp65蛋白,以副结核分枝杆菌C-2株染色体DNA为模板,以hsp65基因的特异性引物进行PCR扩增,获得了1 626bp的hsp65基因,通过T-A克隆技术,将PCR产物克隆至pGEM-T Vector中,以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆,成功地构建出克隆质粒pGEM-T-hsp65,以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列,结果表明,所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致,两者核苷酸序列的同源性为99.1%,氨基酸序列的同源性为99.3%,表明该基因在副结核分枝杆菌中高度保守。

There are difference from partial HGV gene sequence in Henan province to sequence reported. The homology ofChinese isolate genes was higher than that of other countried.

河南株庚型肝炎病毒部分基因序列与已报道的序列均不完全一致,与国内分离株的同源性高于国外分离株。

This thesis discussed three kinds of major technologies to reduce high PAR on the basis of the principle of OFDM system: pre-distortion technology techniques (clipping and filtering, peak windowing and companding technique), code techniques (Reed-Muller coding algorithm of Golay complementary sequence), and scrambling techniques (selected mapping, partial transmit sequence and selective scrambling), these techniques have some certain limitations.

本文在介绍OFDM理论的基础上,专题讨论了抑制高峰值平均功率比的三类主流技术:信号预畸变技术(限幅滤波、窗函数和压扩技术)、信号编码技术(Golay互补序列的Reed-Muller编译码算法)和信号扰码技术(选择性映射技术、部分传输序列技术和选择性扰码)。

Since the concept of NA have a lot of applications in multivariate statistical analysis, reliability theory and percolation theory, various aspects of NA are significant and have been investigated by a host of scholars Fist, we give some Fuk-Nagaev type probability inequalities, moment inequalities of maximal partial sum for sequence of NA random variables, and some strong law of large numbers and complete convergence for sequence of NA random variables are obtained.

在本论文中我们对不要求强平稳或同分布的NA随机变量列进行了多方面的研究:首先对NA列建立了一组具有NA特点的关于最大部分和的Fuk-Nagaev型概率不等式及其关于某一类特定函数的矩不等式,它们在后续给出的极限定理的证明中发挥着重要作用;研究了具有不同分布的NA列的强收敛性,如对NA列建立了更一般条件下的Wittmann型强大数律及完全收敛性,同时也进一步改进了R。

Finally,the WISCONSIN Sequence Analysis Package (GCG,10.2 version),Phylogenetic Analysis Using Parsimony (PAUP,4.0 version) and Treeview (1.5 version) software were used for data analysis,sequence translation,phylogenetic tree drawing and molecular evolution analysis.

本研究结果证明,H9N2亚型人体分离株属于G1-like亚分支的G1-HK小分支,该小分支毒株之间具有很高的同源性,因此该小分支AIV可能具有感染人的可能性,是具有引起人流感暴发潜能的病毒。

MOTIVATION: The rapid growth of DNA sequence data creates a need to find graphic tools to study DNA sequence in a perceivable form.

动机:快速增长的DNA序列数据产生了一个找寻可视化工具,以一个可感知的形式来研究DNA序列的需要。

To begin with, theory of FCSR and its sequence is summarized and investigated. Furthermore, emphasis is placed on the vaule of 2-adic complexity. A conclusion is drawn that the vaule lies in the relation between 2-adic complexity and lineai complexity of a same seqence. Fact that there exists sequence with high linear complexity but low 2-adic complexity is given and analyzed. The value of 2-adic complexity in cryptanalysis can be directly drawn from the very fact. A series of problems are opened based on the value and discussions are done in various depth. Some good results are obtained in these problems such as the 2-adic complexity distribution of periodic sequences and the stability of 2-adic complexity. The value of FCSR in stream cipher design is discussed at the same time.

首先对FCSR及其序列理论进行了系统的总结和研究,然后重点探讨了2-adic复杂度的流密码意义,通过论证表明,存在线性复杂度较大而2-adic复杂度较小的序列类,从而明确了2-adic复杂度的流密码分析意义,进而提出了一系列相关的研究问题并进行了不同深度的探讨,对其中周期序列的2-adic复杂度分布和2-adic复杂度的稳定性等问题得到了较好的结果;同时对FCSR的流密码设计意义进行了探讨,对其中的问题给出了一些思路或简单结论。

From our results we know that the average error of the Lagrange interpolation sequence and the Hermite interpolation sequence based on the Chebyshev nodes in the 1-fold integrated Wiener space equal weakly to the average error of their corresponding optimal approximation polynomial in the 1-fold integrated Wiener space,and as a kind of information-based operation,they have simple form and their recover functions are polynomials,in the 1-fold integrated wiener space,their average error equal weakly to the corresponding minimal information radius whose permissible information operators class is function values.

通过我们的结果可以知道,基于第一类Chebyshev多项式零点的Lagrange插值算子列和Hermite插值算子列在1-重积分Wiener空间下的平均误差弱等价于相应的最佳逼近多项式在1-重积分Wiener空间下的平均误差,并且作为形式简单且恢复函数为多项式的一种信息基算法,其在1-重积分Wiener空间下的平均误差弱等价于相应的以函数值计算为可允许信息算子的最小平均信息半径。

Firstly,the wavelet-based contourlet coding using an SPIHT-like algorithm is utilized on the plain image,and as a result,the compressed datum stream could be achieved which is then mapped to a 3D bit matrix. Afterward,a chaotic sequence is generated by Lorenz chaotic map,and it is preprocessed to a bit sequence which is used to permute and substitute the elements of the 3D bit matrix achieved above. In the end,the processed 3D matrix is mapped back to datum stream,and the encrypted compressed image could be achieved if decoding and inverse transform are performed on the datum stream.

首先采用基于小波的Contourlet变换的类等级树集合分割编码算法对明文图像进行压缩,得到压缩数据流,然后将压缩数据流映射为一个三维位矩阵;利用Lorenz混沌映射产生混沌序列,并对其进行预处理得到比特值序列,根据比特值序列对上述三维位矩阵进行置乱和替代操作;将置乱和替代后的位矩阵重新映射为数据流,并对其进行解码和反变换操作,得到加密后的压缩图像。

According to drilling, logging and seismic data in the north of the basin, four types of identification signs were determined to the Phanerozoic sequence boundary. And two kinds of sequence boundaries (unconformity and shift surface of deposition) were identified.

应用层序地层学的原理和方法,综合研究盆地北部钻井、测井及地震资料,确定4种层序界面识别标志,在盆地北部新生代始新世—中新世识别出2种类型层序界面:不整合面和沉积作用转换面。

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Must have Coordination and negotiation skill, flexible ideas, and be punctilious person.

必须具备较好的协调与谈判能力,头脑灵活,工作认真仔细

Result: 18 patients are all with simple eye.The pigment mole lies in nasal and temple sides of the ball conjunctiva,in which 2 lie in lacrimal caruncle and semilunar plica.

结果:18例患者均为单眼,色素痣大多数位于内外侧睑裂部球结膜面,2例位于泪阜及半月皱襞,经激光治疗后,10例色素痣消失,有5例行再次激光后消失,2例行第三次激光后消失,1例第四次激光后消失。

Buy the home shilly-shally to see when group the person is in when speaking highly of this net inn, absolutely meeting gives up of the apprehension in the heart.

当一个犹豫不决的买家看到一个群的人都在夸赞该网店时,绝对会打消心中的顾虑的。