查询词典 selection function

This review discusses some technological details and problems, and reviews the progresses, including selection and management of competent cells, optimization of preculture and coculture, selection, foreign gene expression and regulation.

本文综述了近几年来此项研究在感受态细胞选择与调节、预培养及共培养体系优化、转化子的筛选及外源基因表达调控等方面的主要进展。

Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究，建立的'新红星'苹果遗传转化体系为：叶片黑暗预培养3天，经农杆菌侵染3分钟，在含TDZ0.5mg/L，NAA0.3mg/L的MS培养基上黑暗共培养3天，然后加250mg/L Cef黑暗维持培养3天，再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现；转为光照条件1-2次的继代筛选，获得抗性芽后，进行扩繁。

Preprocessing consists of data selection, baseline adjustment, noise filtering and peak identification. Data selection removes preprimer data section which contains little useful information. Baseline adjustment adjusts the baselines of four channels to the same level. Noise filtering removes the impulse noise and white noise. Peak identification selects the peak data for use in cross matrix determination.

预处理包括数据段选取、基线调整、噪声滤除、峰值识别四个模块：数据段选取删除前段冗余数据；基线调整使得四色荧光信号的基线高度一致；噪声滤除去除信号中的脉冲噪声和白噪声；峰值识别选取峰值数据，作为求解串扰矩阵的数据。

To obtain efficient survivability, this paper presents a novel restorable lightpath selection algorithm based on the automatically switched optical network frame. A numerical simulation is made in term of this routing selection algorithm. The result shows that the traffic has an obvious balancing trend; the block probability of connection requests has been reduced. At the same time, this algorithm has an excellent performance on bandwidth utility and sharing of the restoration resources.

摘要为了获得高效的网络生存性能，基于自动交换光网络的框架，该文提出了一种新型的可恢复路径选择算法-联合可变权重可恢复路径选择算法，并进行了数值仿真分析，仿真结果表明，此恢复路径选择算法具有明显的业务量均衡能力，并降低了动态连接请求的阻塞概率，同时具有良好的带宽利用率和恢复资源共享效率。

The actuality, characteristic and influence factors of structural form selection in highbuildings is discussed resumptively, and the use of fuzzy generally decision making method in structural form selection in highbuildings is illuminated concretely.

对高层建筑结构选型决策的现状、特点及影响因素作了概括性论述，具体说明了模糊综合评判法在高层建筑结构选型中的应用，并通过一个实际工程。

These were ealuated forCobb angle, end ertebrae selection, Ferguson angle, apex of the cure, C7 balance, pelic obliquity, Risser sign,status of the triradiate cartilage, kyphosis Cobb angle, endplate selection for kyphosis, and kyphotic index.

分别从Cobb角，终椎的选择，Ferguson角，顶椎的选择， C7平衡，骨盆倾斜， Risser征，髋臼&Y&软骨的情况，脊柱后凸Cobb角，脊柱后凸的终板选择，脊柱后凸指数进行测量。

As part of the disagreement about whether natural selection alone was sufficient to explain speciation, George Romanes coined the term neo-Darwinism to refer to the version of evolution advocated by Alfred Russel Wallace and August Weismann with its heavy dependence on natural selection.

是否单独自然选择就足以解释物种形成是争论的一部分；乔治·罗曼纳创造&新达尔文主义&一词，以代表阿尔弗雷德·罗素·华莱士和奥古斯特·魏斯曼所倡导的、强烈依赖自然选择的进化版本。

On the Presuppositions of Group Selectionism DONG Guo-an ( Research Institute of Philosophy, South China Normal University, Guangzhou 510631, China)One principal problem of the debate about level of selection has been the importance of group selection.

关于选择层次的一个主要问题是：群体选择概念在解释诸如利他行为这类性状的进化中是否必要？

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。