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(1) In common cavity malformation, in addition to the abnormal bony labyrinth such as the cochlea, vestibule and lateral semicircular canal, the inner ear perceptive organ such as the saccule, utricle and scala media were also abnormal.(2) Though we could not identify the basal membrane and sensory epithelia under micro-endoscopy, the utricle and saccule could be clearly identified.(3) During cochlear implantation in common cavity malformation, electrode insertion should be monitored under micro-endoscopy, the electrodes should be put close to the anterior wall of the cavity, otherwise the vestibular organ may be damaged.

(1)共同腔畸形极重度聋患者不仅前庭、外半规管和耳蜗的骨性结构形态发生了变化,而且腔内耳蜗中阶与前庭的球囊、椭圆囊的膜迷路结构形态也发生了变化;(2)微窥镜下分辨不出基底膜或听神经细胞的形态结构,可见畸形前庭的球囊、椭圆囊的囊性结构;(3)进行共同腔畸形人工耳蜗植入手术时,应在耳蜗内镜的监视下,将电极摆放到准确位置,尽可能贴近共同腔的前壁,不应损伤前庭器官。

Zheng Mingxun can say is the Seiji Ozawa in Europe after Italy's most particularly in the Asian-command master of La Scala opera house he has a very special feeling.

郑明勋可以说是继小泽征尔之后在欧洲特别是在意大利最红的亚裔指挥大师,他对斯卡拉歌剧院有着非常特殊的感情。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Cortis tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL/6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像;②获得了各种听功能密切相关的结构的成熟时间点,基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14);③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点:④Calbindin在E16以后的螺旋神经元没有表达,但是在scarpa神经元持续表达。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti\'s tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL/6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像;②获得了各种听功能密切相关的结构的成熟时间点,基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14);③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点:④Calbindin在E16以后的螺旋神经元没有表达,但是在scarpa神经元持续表达。

Objective:To determine the position of implanting electrode into the scala tympani.

目的:确定人工耳蜗电极植入耳蜗入口的位置。

It suggests that this anatomic parameter provide a reference value to determine the position of cochlear implant electrode into the scala tympani.

该解剖参数为蜗窗难以找到的人工耳蜗植入病例提供了定位参考。

Results: Substantial gradients of gentamicin along the length of the scala tympani were demonstrated and quantified, aeraging more than 4,000 times greater concentration at the base compared with the apex at the time of sampling.

结果:研究证明并定量了庆大霉素沿着鼓阶长度的实际浓度梯度,在同一时间获取的样本之中,耳蜗基底部庆大霉素的浓度平均较耳蜗顶部的要高出四千多倍。

Methods auditory brain stem response was used to examine before,after and two weeks after fenestration scala tympani respectively in twenty ears of twenty guinea pigs,and the specimens for light microscope and scanning electron microscopy were made respectively.

方法对 2 0只豚鼠的 2 0耳在开窗术前、术后及开窗修复术后 2周,分别作听性脑干反应检查,并制作术后 2周耳蜗的光镜和扫描电镜标本。

Result: 2 cases had successful cochlear implantats, another severe case failed to have the electrode inserted into the scala tympani.

结果:2例病变较轻者成功地进行了人工耳蜗植入术,术后恢复良好,1例因病变严重电极未能植入。

Part three:Cochlear on Hair Cell Like Cells Regeneration in Presbycusis C57BL/6 Mice after Math1 Gene Inner Ear TransfectionIn order to test the probability of Mathl gene induces hair cell like cells regeneration in cochlea of presbycusis C57BL/6 mice,8 mounths old C57BL/6 mice were used for this study and were microinjected with Ad-MathlorAd-EGFP in the scala tympani by ventral approach.

第三部分老年性聋模型小鼠C57BL/6耳蜗鼓阶外源基因Math1导入后出现毛细胞样细胞现象的观察为了观察老年性聋C57BL/6小鼠(8月龄)耳蜗鼓阶外源基因Math1导入后能否在老年性聋的耳蜗微环境诱导出新生的毛细胞样细胞,为Math1基因治疗老年性聋进行探索性研究。

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