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The CryCI gene and essential regulation elements cut from the plasmid pGF4ABC were inserted into yeast expression vector pPIC9K and plant expression vector pBI 121.1, so we got the secretive yeast expression plasmid pPIC9KBC and plant high-efficiency expression plasmid pGBIF4ABC, Fifty-two His+Muts transformants were obtained after the expression vector pPIC9KBC was introduced into Pichia pasoris, KM7I strain, by electroporation.

将pGF4ABC上的融合杀虫基因CryCI分别克隆到酵母表达载体pPIC9K和植物表达载体pBI121.1上，构建成融合杀虫基因的分泌型酵母表达载体pPIC9KBC和高效植物表达载体pGBIF4ABC。

It points out that under the hard-switching PWM pattern, the movement speed of flux linkage is adjusted by zero space voltage vector, but when soft-switching PWM pattern is used, effect time of space voltage vector vary greatly, sometimes even no zero space voltage vector exist.

指出在硬开关PWM模式下，磁链的运动速度靠零空间电压矢量调节；而在软开关PWM模式下，空间电压矢量的作用时间发生了很大变化，有时甚至没有零空间电压矢量。

A new SVM method suitable for four-switch inverter fed PMSM DTC system was proposed. By analyzing the property of the equivalent zero space vector and its imitation scheme in detail, the paper introduced a zero-vector partition idea, which is usually adopted in the SVPWM modulation, to the composition of desired space vector.

该文针对四开关逆变器供电PMSM DTC系统的特殊性，采用了一种新型SVM方法，分析了等效零矢量模拟技术，将传统SVPWM调制中的劈零思想引入到空间矢量的合成上。

Experimental results show that the zero effort miss vector calculated by using approximate formulas is close to the real miss vector. Under the conditions of small initial errors, the satisfied results can be obtained by approximate function. The zero effort miss vector obtained can be used for the preliminary design and analysis of kinetic interceptor guidance.

测试结果表明该近似算法得到的零控脱靶量与真实值比较接近，在较小初始偏差条件下，简化的近似计算公式能给出满意的结果，所得零控脱靶量可用于空间动能拦截制导系统的初步设计与分析。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

This algorithm constructs a 64-D crossband vector according to the characteristics of the decomposed image, and adopts nonlinear interpolative vector quantization and soft computing based codebook generation algorithm. Hence, this algorithm can improve the vector quantization performance significantly from both aspects of speed and precision, and it can get a high reconstruction quality (PSNR＞34dB) with a very low bit rate (0.172bpp).

该方案依据图象小波变换的特点构造了64维的跨频带矢量，并采用了非线性插补矢量量化和柔性计算码书生成算法，从速度和精度两方面提高矢量量化的性能，从而在极低比特率的条件下(0.172bpp)获得了高质量的重构图象(PSNR＞34dB)。

The three conserved histidine residues with a conserved glutamate residue fits the active site of the homology model of oxalate oxidase.2. Construction of plant binary expression vector of germin geneThe germin gene fragment cut with BamH I from intermediated vector pDG28 was inserted into binary vector pBI121 cut with the same enzyme to formrecombinants which was selected by plaque PCR .

Germin基因植物表达载体的构建将中间载体上的germin基因片段用Bam H I酶切下来，插入用同样酶切的双元表达载体pBI121上，以菌落PCR筛选重组子。

This method uses what Professor Salton has proposed as the vector spacial model to express text as an invisible vector in hyperspherical space.It makes use of certain pre-disposal methods (reduce the dimensions,clustering thesaurus,filtering out old-fashioned words) to reduce the dimensions.It usescosines to measure the degree of similaritybetween texts and uses the total similarity as the target convergence vector.It produces clustered results by using restricted iteratives to achieve partial perfection.

该算法用Salton教授提出的向量空间模型把文本表示成不可视的超球向量空间的一个向量，采用一定的预处理手段(降维、同义词归并、停用词过滤)完成维数约减，使用余弦相似度度量文本之间的相似度，并选择总凝聚度作为目标收敛函数，通过有限次的迭代达到局部最优，输出聚类结果。

There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式，可以避免教学形成模式後的僵化现象。