查询词典 restriction

This thesis focus on GPS application in magnetic suspension engineering:① the formulation of oblique cylindrical projection is worked out according to characteristics of long distance precise engineering and map projection theory. The author has developed an application to accomplish coordinate conversion from generally used coordinate systems to oblique Maglev Coordinates System.② considering not only project peculiarities but also position of control points, selection of initial points, connection type, selection of repeat baseline and ground observed quantities, GPS network is designed on electronic map and we may extract GPS network information by program developed using Visual Basic 6.0 while the results are stored in control point file and baseline vector file.③estimate accuracy of various GPS networks designed on electronic map by method of 2D restriction adjustment. GPS network simulation design and accuracy estimate come true.④Predominate the rules how connection type , repeat baseline and precise baseline of range finder influence GPS network precision through comparing accuracy of various GPS network. Thereby, the solution how to establish GPS network in long distance precise engineering is figured out.

本论文主要研究GPS技术在磁悬浮工程中的应用：①根据长距离精密工程的特点，从地图投影原理出发，推导出斜轴圆柱投影的应用公式，并编制计算软件，实现从常用坐标系统向斜轴磁悬浮坐标系统的转换计算；②在AutoCAD电子地图上，根据工程的需要，并充分考虑控制点的点位、起算点的选择、连接方式、重复基线的选择和地面观测量的选择等因素，在电子地图上进行GPS网的设计，根据Visual Basic 6.0语言编程，提取出GPS网资料，保存在控制点文件和基线向量文件中；③对在电子地图生成的各种类型的GPS网进行二维约束平差的精度估算，实现了GPS网的模拟设计与精度估算；④通过对各种控制网的精度比较，掌握连接方式、重复基线、精密测距仪基线对GPS网精度影响的规律，解决了长距离精密工程GPS网的建立方法。

Under the condition of the restriction and obscurity, idea is not accurate.

在这样的限制及障碍下，念头的精准性是有问题的。

While there is no age restriction to buy, occupant has to be 55 years of age or older.

以上所有的中文翻译如有不完善及错误的均以英文版的说明为准，敬请见谅H

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

The swimming club is open to families in the neighborhood without restriction .

这个游泳俱乐部对邻近地区的家庭开放，不受限制。

The swimming club is open to families in the neighborhood without restriction

这项工程耗资在两百万美元左右。

The swimming club is open to families in the neighborhood without restriction.

這個游泳俱樂部對鄰近地區的家庭開放，不受限制。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

With the restriction of water demand and supply, the objective function optimal value was calculated by sequential quadratic programming.

以需水量和可供水量为约束条件，采用序列二次规划法求解函数最优值。

Methods Polymerase chain reaction and restriction fragment length polymorphism was used to identify mdm2 genotypes. The Pearson Chi square test and Woolf statistic method were used to analyze the relative risk and 95% confidence interval in order to find the genetic association between polymorphism of mdm2 gene and symptoms and pathological types of NSCLC.

采用聚合酶链反应和限制性内切酶片段长度多态性方法检测88例NSCLC患者的mdm2基因型，并通过Pearson卡方检验及Woolf方法计算相对风险度及95%可信区间（95%CI），验证mdm2基因多态性与各临床症状及病理类型之间的关联性。

These are places without aristocratic baggage; egalitarian places open to talent, self-improving, engaged in learning and innovation through networks that were at once competitive and cooperative.

这些地方没有贵族遗风作祟，而且对于那些有天分的人是开放的平等之地，这些人善于通过那些曾经很具有竞争力与合作精神的关系网进行自我提高以及学习创新。

Christine: You don't want to see me?

你不想见我？