查询词典 pured

The c oaventioaal method was improved by using agarose as supporting medium instead pured agar and adopting pre-electrophoresis in order to make it more sensitive.

笔者在常规方法的基础上，做了些改进：用琼脂糖代替纯化琼脂；并先将诊断血清加在阳极端孔内，预电泳15—20分钟，然后将待检标本加入阴极端孔内继续电泳50分钟。

The analysis of scanning electromicroscope showed that it didn't obviously exist protein granules in rice starch pured by alcalase, and the solubility and swelling power of rice starch increased respectively after purification.

扫描电镜分析大米淀粉的超微结构显示，碱性蛋白酶纯化处理后的大米淀粉中未见明显的蛋白质颗粒存在。比较纯化前后的大米淀粉发现，大米淀粉经过碱性蛋白酶纯化后，其溶解度和膨润力都明显增加。

The analysis results of scanning electromicroscope showed that protein granules didn't obviously exist in rice starch pured by alcalase, and the solubility and swelling power of rice starch increased remarkably after purification.

扫描电镜分析大米淀粉的超微结构显示，碱性蛋白酶纯化处理后的大米淀粉中未见明显的蛋白质颗粒存在。比较纯化前后的大米淀粉发现，大米淀粉经过碱性蛋白酶纯化后，其溶解度和膨润力都明显增加。

Methods 10 adult arms were pured latex into artery microanatomized and observed the dorsal branch of anterior interosseous artery's originate、diameter、length and distribution of skin branch and periosteum branch.

10侧成人上肢标本经肱动脉灌注乳胶后，对其骨间前动脉背侧支的起始部位、口径、长度及其皮支、骨膜支的分布进行显微解剖观察。

The pured mammary epithelial cell line could be obtained by 2~3 passages. 2.Using RPMI1640 containing 10黃, 100IU/ml Penicillin and 100IU/ml Streptomycin as basic media, several different growth factors such as insulin, hydrocortisone, insulin-like growth factor, EGF and so on, were added and selected to culture goat mammary epithelial cells.

以RPMI1640 为基础培养液，山羊乳腺上皮细胞在含血清、双抗的同时，添加氢化考的松、Insulin、IGF-1、E2、EGF 及ITS 的不同组合，以细胞生长群体倍增值为参考标准，评估不同组合的生物活性因子对山羊乳腺上皮细胞生长的影响。

Methods first , to acquire the highly pured glomeruli by three steps grade filtrated net ; next , to culture the microvascular endothelial cell in vitro with the transplant methods of isolated glomerali.

方法采用三步梯度筛网法，先获得较高纯度的肾血管球，再应用肾血管球外植法体外培养肾微血管内皮细胞。

The pured mammary epithelial cell line could be obtained by 2~3 passages. 2.Using RPMI1640 containing 10%FCS, 100IU/ml Penicillin and 100IU/ml Streptomycin as basic media, several different growth factors such as insulin, hydrocortisone, insulin-like growth factor, EGF and so on, were added and selected to culture goat mammary epithelial cells.

以RPMI1640 为基础培养液，山羊乳腺上皮细胞在含血清、双抗的同时，添加氢化考的松、Insulin、IGF-1、E2、EGF 及ITS 的不同组合，以细胞生长群体倍增值为参考标准，评估不同组合的生物活性因子对山羊乳腺上皮细胞生长的影响。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Methods:Cardiac muscle of twoday neonate rattus was digested repeatedly by 0.0625% trypsin.The cardiocytes were collected in DMEM medium,which contains 10% fetal bovine serum.Myocardial cells were isolated with the technique of differential anchoring velocity with 90 min and were pured by Brdu.The cardiocytes were placed into CO2 incubator to incubate seven days after their purification.

应用0.0625%的胰蛋白酶重复消化出生第2 d乳鼠的心肌组织多次，收集的细胞用含10%胎牛血清的DMEM培养基中和，用差速贴壁分离法分离，在以溴脱氧尿嘧啶纯化心肌细胞后置CO2培养箱孵育7 d。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

We are forbidden from giving money to these shoeless, skinny children, as this will perpetuate begging.

向光着脚，骨瘦如柴的孩子施舍是不可以的，因为要招来无休无止的乞讨。

It would be hard not to fall victim to some sharpeyed policeman.

要想不被某个目光锐利的警察抓到，谈何容易。