查询词典 proliferation

MAIN OUTCOME MEASURES: Proliferation and osteogenic activity of cells were measured by morphology, ultrastructure, and cell proliferation curves, analyzed by calcification nodi Von Kossa staining, and determend by quantitative analysis of contents of cytoplasmic alkaline phosphatase and amendable calcium-cobalt staining.

主要观察指标：通过形态学、超微结构，细胞增殖曲线，钙结节Von kossa法染色以及细胞内碱性磷酸酶定量检测与钙钴法染色确定其增殖与成骨活性。

Through a study on tissue cultureof the bud scale of corm of Lili longiflorum separated from the plant ,several practical TC prompt proliferation techniques ,such as inducing of lush bud ,proliferation cultivation of successive generation, cultivation of growing roots and transplanting , etc., are researched in this paper .

通过对百合球茎鳞片离体组织培养的研究，探讨了丛生芽诱导、继代增殖培养、生根培养及移栽等方面的组培快速繁殖以及生产上实用的组培技术。

Result s:10 μmol/L retinoids could inhibit the Bel-7402 proliferation and decrease the colony forming of liver cancer cells on soft agar significantly.So some biochemical markers indicating the proliferation status of liver cancer cells changed significant ly, including the significant activity increase of ornithine carbamyl transferase, tyrosine-α-ketoglutaric transaminase and alkaline phosphatase, the significant decrease α-fetoprotein secretion,γ-glutamyltranspetidase and aldolase activity.

结果：10μmol/L维甲酸显著抑制肝癌细胞增殖，并使肝癌细胞软琼脂集落形成率明显减少，使代表肝细胞分化的酶鸟氨酸氨基甲酰转移酶、酪氨酸-α-酮戊二酸转氨酶和碱性磷酸酶比活力明显升高，甲胎蛋白分泌量、γ-谷氨酰转肽酶和醛缩酶比活力明显下降。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The recombinant eukaryotic expression vector pcDNA3.1-Myc-His B/tPA can stable express tPAcDNA in transfected hUVEC cell strains and can secret compt tPA protein. Experiment III The Effects of Transfected tPA Gene on Vascular Smooth Muscle Cells Proliferation and Migration in VitroObjective To investigate the effects of transfected tPA gene on vascular smooth muscle cell proliferation and migration in vitro and to provide a basis for further study on preventing vein graft restenosis.

结论构建的pcDNA3.1-Myc-HisB/tPA基因新型真核表达载体，能在HUVEC中表达，其表达具有高拷贝、稳定性好的特点，获得的表达蛋白活性较高，提示本载体和稳定表达tPA基因的人脐静脉内皮株能够作为基因治疗工具用于哺乳动物的实验研究。

The result show in transplanted vessel EF-1 and PCNA are mainly expressed in smooth muscle cells and Clemons rate proliferation of SMC is the core of endothelium proliferation.In the third part of our experiment we use fluid nitrogen to fire transplanted rein, crofter tissue homogeneous to test the expression of CE and EF-1 gene we use RT-Craned immunohistologiccal method to test mKNA and protein level respectively and analyze the expression of these tm genes in transplanted vein.

本实验的第二部分，我们将术后大鼠分为10组，每组8只，分别于术后6hours、24hours、72hours、1W、10days、2W、3W、4W、6W、8W切取移植静脉，将切取的不同时期的移植静脉分别置于10％中性福尔马林溶液中固定和液氮冷冻固定，将福尔马林溶液固定的标本制成4μm厚石腊切片，，常规进行病理观察大体改变，静脉标本HE染色后于光镜下观内膜增殖情况，并应用PCNA单克隆抗体，观察SMC增殖情况。

Methods: We used virus proliferation assay cell viability assay to evaluate the proliferation and cytolysis selectivity of CNHK500. And we used Western-blot to confirm the expression of adenovirus CNHK500 E1A and E1B in cancer and normal cells.

行病毒增殖实验和细胞生长抑制实验，验证CNHK500选择性复制和杀伤能力；Western blot检测腺病毒E1A和E1B在细胞中的表达。

Methods: The telomerase activity in breast cancer cells and MRC-5 cell line were detected semi-quantitively by TRAP-ELISA assay. Virus proliferation as- say and cell viability assay were used to evaluate the proliferation and cytolysis selectivity of CNHK300, and the results were compared with those of wtAd5. The breast cancer cells were treated with CNHK300 combining paclitaxel to evaluate the killing effect of the combined regimen.

用TRAP-ELISA方法检测乳腺癌细胞株(MDA-MB-453、SK-BR-3)和正常成纤维细胞株MRC-5的端粒酶活性；进行病毒增殖实验和细胞生长抑制实验，并与野生型腺病毒wtAd5进行对比，验证CNHK300选择性复制和选择性杀伤能力；用细胞生长抑制实验进一步考察CNHK300与化疗药物紫杉醇联合应用对乳腺癌细胞的杀伤作用；用Western blot检测腺病毒E1A在细胞中的表达。

Doxy treatment significantly reduced intimal SMCs proliferation from 62.76%±1.02% in the controls to 43.23%±1.06% at 7 days after injury (P.01). CONCLUSION: Doxy treatment inhibits the activity of MMPs, the SMCs proliferation of intimal, neointimal hyperplasia and vascular remodeling, suggesting that Doxy treatment is useful in preventing restenosis after PTCA.

强力霉素可以显著降低血管损伤后MMPs活性，抑制内膜平滑肌细胞的增殖、新生内膜增生以及管腔重构，提示它可能具有防治PTCA术后再狭窄的作用。

Results: 48 h after treatment, most of the patients had a marked improvement in irf and ret (p＜0.05), and irf showed a more significant increase. moreover, the value before treatment and 48 h after treatment of irf had no overlap; irf and ret were further increased after 1 week; after 12 weeks irf%, hct, ret, rbc and hb were significantly increased than before treatment, bone marrow erythrocytic series proliferation was more active than that before treatment, each kind of erythroblast ratio and the percentage of total erythrocytic series were significantly increased, especially late erythroblasts proliferation.

结果：治疗过程中大部分患者在epo注射48 h后irf、ret升高（p.05），irf升高更为显著，且irf在给药前和给药48 h后的数值无重叠区，1周后irf、ret进一步升高，12周时irf、hct、ret、rbc、hb均比用药前显著增高，骨髓红系增生较治疗前明显活跃，各类幼红细胞比率及总红系所占百分数均显著升高，其中以晚幼红细胞增生最为明显。

It was just a normal day of school

那只是上学的普通一天

Which is a very good reference in this issue.

这是一个在这个问题上很好的参考。