查询词典 preparation by screening

Preparation of monoclonal antibody to human augmenter of liver regeneration : screening of hybridomas with unpurified antigen expressed by E coli .

人肝再生增强因子单克隆抗体的制备：以非纯化的大肠杆菌表达抗原筛选杂交瘤。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

Objective: On the basis of the random mutagenesis library of ARS, we wish to preparation by screening new ARS sequence which not only have higher ARS activity, but also display higher transformation frequency, in order to constructed new gene recombination vector which have higher efficiency in the Nonconventional yeast expression system.

目的：通过构建酵母ARS 随机突变体库，从中筛选出具有较强的转化效率的ARS序列，用以构建新型高效的克鲁维酵母基因工程载体。

Based on the screening of chlorpyrifos biodegrading fungal strain, Cladosporium cladosporioides (the ID of fungi preservation CCTCCM207111. Accession number of ITS sequence analysis Genebank EF405864), extracellular enzymes were extracted. Applying the composite rotatable design, a model of the optimal mixture protect agents was established. After the optimal composition was validated, the enzyme preparation storage experiment was tested to determine the stability of biodegradation by high performance liquid chromatography analysis.

在自主筛选毒死蜱高效降解真菌Cladosporium cladosporioides（菌种保藏号CCTCCM207111，ITS序列分析GeneBank登陆号EF405864）的基础上，发酵提取其胞外酶，采用&通用旋转回归法&筛选了酶活性保护剂的最佳配方，并进行验证；利用所得配方进行制剂贮藏实验，利用HPLC检验其降解性能稳定性。

Graf, without question, is the greatest women's player in history.

格拉芙，现在，毫无疑问是女子网坛历史上最伟大的选手。

If not, you will have to look around at all your important data.

如果没有，您将需要检查所有的重要数据。