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In fact, this procedure is the preceding one of the handwritten digit recognition.

其实，这个阶段也就是手写数字识别的前处理阶段，为手写数字的识别做一些准备工作。

With regard to the satisfaction of perceived need, there are six items out of the preceding ten diverse from both sides. When divided into five categories, the distributions between two sides are opposite in each category.

在比较各项需要是否获得的认定上，前十项需要中护理人员有六项与家属的不同；比较五大类需要是否获得的认定上，两者的结果则不太相同。

It utilises advanced metallurgy techniques, superior construction and better trained crews to outrange and outshoot nearly all preceding bombards.

得益於先进的冶金技术和火炮设计，以及训练有素的炮兵部队，长管炮无论射程还是威力都几乎可压制任何先前火炮。

Based on indicators like (1) global real estate overvaluation,(2) indebtedness,(3) leverage,(4) outstanding derivatives,(5) global bubbles, and (6) the precariousness of the global monetary system, I would argue that the accumulated imbalances in the current period surpass significantly those preceding the Great Depression.

基于指标像（ 1 ）全球房地产过高，（ 2 ）负债，（ 3 ）杠杆，（ 4 ）杰出的衍生物，（ 5 ）全球泡沫，和（ 6 ）不稳定的全球货币体系，我认为，所积累的不平衡，本期大大超过之前的那些大萧条。

Holy Week, in the Christian liturgical year, is the week immediately preceding Easter, beginning with Palm Sunday.

圣周，在基督教教会礼仪年，今年已是一周前的复活节开始，棕榈周日。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

"The preceding chapter, for the most part, dealt with paraxial theory as applied to thin spherical lens systems."

前一章讨论的基本上是应用于薄球面透镜系统的傍轴理论。

The diff above shows the differences between the working-tree version of file.txt and the stage 2 and stage 3 versions. So instead of preceding each line by a single "+" or "-", it now uses two columns: the first column is used for differences between the first parent and the working directory copy, and the second for differences between the second parent and the working directory copy.

上 述的输出显示了 file.txt 文件在工作树中的版本对阶段2（stage 2）与阶段3（stage 3）中的差异，并且在每行中加上"+"或者"-"的前缀，分两列排列：第一列用于标记第一个父交付对工作树的差异，第二列用于表示第一个父交付对工作树的差异。

Even if we do not allow that the immediately preceding clauses are parenthetical to the main thought of this passage – a position that has much to commend it – there should be no difficulty with the construction.

就算我们不接受，22节下和23节是一个括弧，也不应该因造句而感到困难。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。