查询词典 polymorphism

Methods The polymerase chain reaction-restriction fragment length polymorphism was used to analyze the gene polymorphism of ApoE in 25 high carotid artery IMT patients, 25 carotid artery sclerostenosis patients and 25 healthy age-matched controls.

应用聚合酶链反应-限制性片段长度多态性技术，对25例B超检测颈动脉内中膜增厚患者和25例颈动脉粥样硬化性狭窄患者及25例同期健康体检者的ApoE基因多态性进行分析，观察其多态性。

By the method of polymerase chain reaction-single strand conformation polymorphism, this paper studied the genetic polymorphism of exon 1 and intron 1 in the aquaporin gene of seven species of Stipa in the meadow steppe, typical steppe, and desert steppe of Inner Mongolia.

以分布于内蒙古的草甸草原、典型草原、荒漠草原上的7种针茅属植物为材料，利用聚合酶链反应－单链构象多态性方法，分析了水孔蛋白基因的外显子1和内含子1的遗传多态性。

Objective: The purpose of this study is to investigate the relationship between the insertion/deletion polymorphism of the angiotensin- concerting enzyme gene and congenital heat disease and to ques the predisposing gene of congenital heart disease by analyzing the polymorphism of the ACE gene of the patients of congenital heart disease. So that we can intervene earlier and reduce the incidence of congenital heart disease.

目的 通过对先天性心脏病患儿血管紧张与转换酶基因多态性的分析，探讨血管紧张素转换酶基因插入/缺失多态性与先天性心脏病的关系，寻找与先天性心脏病发病有关的易感基因，以便及早进行干预，减少先天性心脏病的发病率。

Methods: 70 patients with COPD and 70 healthy volunteers were enrolled in this study. Limosis peripheral blood was taken and whole blood corpuscle genomic DNA was extracted. The genetic polymorphism of SOD1-3 genes was determined by polymerase chain reaction and restriction fragment length polymorphism.

收集70例COPD患者和70例健康人空腹外周血，提取全血细胞DNA，采用聚合酶链式反应－限制性片段长度多态性法检测两组SOD1-3基因的多态性。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

Twelve microsatellite markers selected from the GENE-BANK were analyzed for polymorphism in silkies. Allele frequencies were estimated based on band presence or absence. Average heterozygosity and polymorphism information content were calculated by allele frequencies, and then, linkage analysis between character and markers by least squares means and the correlation among the 6 characters of egg traits in silkies were studied.

本试验选用12对微卫星引物，通过对丝羽乌骨鸡多态性扩增，计算出这些微卫星标记座位的等位基因频率、多态信息含量、群体杂合度；通过标记多态性与产蛋性状的最小二乘分析和多重比较，进行了分子标记与产蛋性状的相关分析，同时计算了产蛋性状间的表型相关。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

The single-strand conformation polymorphism method was used to analyze the polymorphism in 3'-flanking region of the HSP70 gene.

同时设计引物扩增 HSP70基因3'-侧翼区，通过PCR-SSCP技术分析 HSP70基因3'-侧翼区的多态性，并发现一个多态位点。

The single-strand conformation polymorphism method was used to analyze for polymorphism at the 5'-flanking region of the HSPA1A gene.

设计引物扩增HSPA1A基因的5'-侧翼区，通过PCR-SSCP技术分析启动子区的多态性，寻找SNPs位点，分析发现：扩增片段大小280bp，扩增产物具有基因多态性。

Extensity, abstractness, polymorphism and space time are their basic feature.

从而，明确了研究工作的基础是对空间数据的定义、存储和处理。

It was just a normal day of school

那只是上学的普通一天

Which is a very good reference in this issue.

这是一个在这个问题上很好的参考。