查询词典 plus strand

To address this issue, we investigated the RNA synthesis in vitro from RNA templates corresponding to the 3\' terminuses of HCV positive- and negative-strand RNA by a purified HCV NS5B.

本论文利用纯化的HCV NS5B蛋白以及HCV正链、负链3'末端RNA，在体外条件下研究NS5B利用两种模板进行RNA合成的情况，以探讨HCV基因组模板选择性的机制。

OBJECTIVE : to analyse the replication activity of NS5B proteins of HCV genotype 1a and 1b when use 3'terminuses of negative RNA strand from HCV 1a, 1b and 2a as templates by performing RdRp assay in vitro and offer some useful data to optimize the model for the screening of anti-HCV drugs targeting at NS5B.

目的：通过体外的RNA依赖的RNA聚合反应，分别比较HCV 1a和1b型来源的NS5B蛋白以不同HCV基因型负链3'末端RNA为模板时的复制活性，为优化以NS5B为靶点的抗HCV药物筛选模型提供理论依据。

The DNA sequences corresponding to the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand were cloned to the transcription plasmid pGEM3Zf.

以1a型负链3'末端RNA为模板，NS5B1和NS5B2为聚合酶，进行体外的RNA合成反应，斑点杂交和RT-PCR检测产物，确定纯化的蛋白具有RdRp活性。

The 3'terminuses of negative RNA strand from 1a, 1b and 2a were prepared via in vitro transcription, using these RNA as temlates and the two purified NS5B proteins as polymerase respectively we conducted the RdRp assay in vitro.

分别以体外转录的HCV 1a、1b和2a型负链3'末端RNA作为模板RNA，NS5B1和NS5B2为聚合酶进行体外RdRp反应，Northern blot检测产物合成情况。

The purified proteins had RdRp activity, and could synthesize RNA products similar in length to the RNA templates using the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand.

纯化的NS5B蛋白具有体外RdRp活性，能够以HCV负链3'末端RNA为模板体外合成一条与模板大小相近的产物。

Each RNA polyerase transcribes only one strand of a double-stranded DNA template.

每种RNA聚合酶只转录双链DNA模板中的一条链。

She twirled a strand of hair round her finger.

她把一绺头发卷绕在手指上。

Step 2: The intervening single strand DNA of 28 nts is then removed after DNA helicase unwinds the DNA.

步骤2：两个切口之间长度为28个核苷酸的单链DNA片断在DNA酶的作用下被移除

The RNA polymerase unzips a small portion of the DNA helix exposing the bases on each strand.

RNA聚合酶解开一小段DNA螺旋，使碱基在它们各自的链上暴露出来。

This year, it's a triple-strand necklace with amber-colored citrine.

今年布什送给劳拉的生日礼物是一条琥珀色的三排珠水晶项链。

I want to be a big fish in a small pond.

我要做一条在小池塘里的大鱼。

While the software functioned well, we did not feel the same about their customer support!

虽然该软件运行良好，我们没有同样的感觉他们的客户支持！