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Sarcomeric actin expression was positive on day 10 after differentiation. the positive expression was in plasma. on day 6, plasma and organelle in cells increased, tight junction and intermediate junction were found and there was apophysis in cell surface and microfilament in apophysis.

电镜观察,未分化细胞为圆形或椭圆形,细胞间连接松散,细胞质较少,细胞器不发达;诱导后6 d,细胞间出现紧密连接和中间连接,细胞质增多,部分细胞表面出现突起,突起内可见微丝。

Blood were obtained for blood RT and plasma endotoxin concentration check. 6 All data underwent ANOVA analysis. Results: After 3hrs of LPS injection, 1 in hypermetabolic sepsis group, the average HR went to 334/min, the average body temperature went to 41.0℃, while in control group they were 274.9/min and 38.2℃ respectively. p=0.000, presented significant statistical differences. The average BP and breath rate in HS group were 97.1mmHg and 94.3/min , while they were 95.9mmHg and 76.4/min in control group, respectively presenting no statistical differences. 2 In HS group plasma endotoxin concentration was 0.088EU/ml while it was 0.013 EU/ml in control group presenting statistical significance, p=0.002. 3 Blood white cell ratio and total white cell counting were 82.73% and 1.90×109 in HS group, while they were 64.39% and 7.58×109 in control group presenting very significant statistical differences(p=0.001). There was no statistical significances in RBC counting and hemocrit between two groups.

结果:LPS持续灌注3h后,结果如下:1)高代谢脓毒症组的心率升高至334.0次/min、体温升高至41.0℃,而对照组则分别为274.9次/min和38.2℃,两组间有显著的统计学差异,p=0.000;血压在高代谢脓毒症组是97.1mmHg,而对照组为95.9mmHg,两组间无统计学差异;脓毒症组的呼吸加快至94.3次/min,对照组则提高至76.4次/min,两组间无统计学差异(p=0.212);2)其血浆内毒素含量为0.088EU/ml,而对照组为0.013 EU/ml,两组间有显著的统计学差异,p=0.002;3)血中性粒白细胞比值升高至82.73%,对照组则为64.39%,两组间有显著的统计学差异(P=0.001);白细胞总数则明显下降至1.90×109/ml,而对照组则为7.58×109/ml,p=0.001,两组间有显著的统计学差异;两组间的红细胞数及血细胞比容均无统计学差异。

In this thesis, the heterojunction with intrinsic hydrogenated amorphous silicon thin layer ( heterojunction with intrinsic thin layer: HIT) solar cells are investigated. The solar cell structure of p/i/n are fabricated. The substrate is a typical 4-inch n-type wafer with (100) crystal orientation, 1~10 ohm-cm and about 525 μm thickness. The p and i a-Si:H layers are deposited by a 13.56 MHz plasma enhanced chemical vapor deposition. The i a-Si:H layers are especially deposited by pulse-wave modulation plasma technique with fixed plasma turn-off time toff = 10 ms and changed plasma turn-on time ton= 10 to 40 ms to selectively control the different silicon to hydrogen bonding configurations. The effect of various Si:H bonds on the performance of HIT solar cells are explored.

中文摘要本论文针对异质接面薄本质层(heterojunction with intrinsic thin layer: HIT)太阳电池进行研究,采用一般典型四英吋晶向为(100,厚度约为525 μm,电阻率约为 1~10 ohm-cmn型矽晶圆为基板,制作p/i/n太阳电池,p及i层薄膜以13.56 MHz电浆加强化学气相沈积,i层并采用脉波调变电浆技术,在固定电浆关闭时间toff = 10 ms及改变电浆开启时间由ton = 10 ms 至40 ms条件下制作具有不同矽氢键结微结构的氢化非晶矽薄膜以探讨不同矽氢键结对太阳电池性能的影响。

The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外,还可见到多种形态异常的虫体,包括;虫体呈非典型二分裂;滋养体胀大,胞质中有团块状和不规则形状的嗜酸性物质;在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛;在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛;在一个母体细胞中含有3或4个子体细胞的雏形;胞质互相融合的4个滋养体的雏形;胞质互相触合的3个滋养体,胞质互相融合的4个滋养体;1对营二分裂的滋养体与另一个滋养休互相融合;2对营二分裂的滋养体互相融合;仅有1个细胞核的滋养体。

The cancers studied here were throat cancer, aute non- lymphoblas He leukem ia and acut lymphoblastic leukemia. The result s indicated that,(1)the levels of Leu, Lys, Ser Asn,Ile,Gly,and Thr were obviously decreased whea lse the levels of orn, Trp evidently increased in cultural liquid of throat can cer cell line indicating that the growth of HepⅡ cell consumed 7 amino acids and releaced 2 amino acids into cultural liquid.(2)the levels of Glu, Gls, Leu, Phe, Tyr and Trp were evidently increased while the levels of Thr , His, Ala decreased in plasma from patient with ANLL. These r esults were consistent with the previous report.(3)the levels of Gly, Trp and Phe in plasma from patients with ANLL evidently de creased and the levels of Ala, His increased after therapy indicating that cance r cells were growing in an anaerobic catabolism state.

这些癌肿包括喉癌HepⅡ细胞,急性非淋巴细胞白血病和急性淋巴细胞白血病,结果表明:(1)喉癌细胞株培养过程中亮氨酸、赖氨酸、丝氨酸、天冬酰胺、异亮氨酸、甘氨酸以及苏氨酸等水平明显降低,而色氨酸水平明显增加,说明喉癌细胞的生长繁殖必须依赖以上7种氨基酸同时释放了色氨酸;(2)急性非淋巴细胞白血病患者血浆中的谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸等水平明显升高,而苏氨酸、组氨酸、丙氨酸等水平明显降低,这些结果与国际报道相一致;(3)经治疗后,ANLL患者血浆中甘氨酸、色氨酸和苯丙氨酸等水平明显降低,而丙氨酸、组氨酸等水平明显升高,表明肿瘤细胞处在无氧代谢。

Microtubules and their textural composite hydrate system, nuclear envelope inside the cell, the places of plasma membrane infolding and cell junction and cell adhesion outside the cell possess lots of nano-scale (100-103 nm) narrow spaces.

在细胞内部的微管及其织构复合水化体系、核膜以及在细胞外部的质膜内褶和细胞连接粘连之处都可以发现许多纳米尺度(100~103 nm)缝隙空间。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明,AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化;TUNEL检测呈阳性,说明有染色体DNA出现断裂;Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露;电镜观察的结果显示,转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

Methods Hexamethyldisiloxane polymer film was obtained by plasma polymerization method on coverslips. Oxygen (O2) plasma treatment was employed to modify the surfaces with different time so that a series of surface with different wettability were produced. L929 cell were cultured on these surfaces to observe the cell attachment rate and cell spreading in 6 and 12 hours.

使用薄膜等离子体聚合法在圆形盖玻片表面生成六甲基二硅氧烷(Hexamethyldisiloxane, HMDSO)薄膜聚合体,然后用低温氧等离子体轰击聚合体表面,根据轰击时间的不同形成一系列的不同润湿性表面,将成纤维细胞系L929接种于不同润湿性表面,观察6h、12h时细胞的贴附率,并观察细胞的形态。

The cell in acidic situation presented a degenerative chang-ment,such as an increased lipid drop and empty cavity in the cell plasma,a py-knotic nuclei,the dilatation of the endoplasmic reticulum and Golgi saccules,mi-tochondrial swelling the number of ribose decrease,and the ribose dissociated inthe plasma,a lot of swallowing cavity,the fibric material decreased accompany-ing with the acid being stronger.

随培养基pH逐渐下降,上述过程因受酸性阻抑而延缓,细胞增殖速度也受影响,处于酸性环境中的软骨细胞呈退行性变化,表现为细胞浆内脂质颗粒与小泡增多、细胞核皱缩、核仁浓染、内质网线粒体膨胀、核糖体数目减少,且与粗面内质网结合的少,游离在胞浆中的多。

Transfer cell A specialized type of plant cell in which the cell wall forms protuberances into the cell, thus increasing the surface area of the wall and plasma membrane.

传递细胞:植物的一种特化细胞,其细胞壁形成突起深入细胞内部,因此增大了细胞壁和细胞膜的面积。

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