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Methods Peripheral blood mononuclear cells from healthy donors were induced to become dendric cells and CIK, using tumor antigen extracted from gastric cancer SGC7901 cells to sensitize DC, antigenpulsed dendritic cells were cocultured with CIK to generate CIK induced by gastric cancer antigenpulsed DC.

从正常人外周血单个核细胞诱导DC、CIK细胞，用胃癌细胞SGC7901抗原致敏DC。致敏DC与CIK共培养，制备经胃癌抗原致敏的DC诱导CIK细胞。

Objective To investigate the variance of dendritic cells from monocytes of peripheral blood in patients with prime nephrotic syndrome.

目的：探讨树突状细胞的数量及功能在原发性肾病综合征时的变化及意义。

Objective: To explore the fine method of isolated cultivation of dendritic cells from the peripheral blood.

目的：探讨无血清专用培养基从人外周血单个核细胞分离培养树突状细胞的优化方法。

Results MMP-2 positive cells are localized in peripheral columnar while no positive cells are seen in odontogenic karatocysts and dentigerous cysts.

结果 成釉细胞瘤中MMP-2阳性染色位于肿瘤外周柱状细胞的胞浆中，中心星网状细胞未见表达；角化囊肿和含牙囊肿的上皮细胞中均未见MMP-2的阳性表达。

A total of 150 mesenchymal neoplasms of the GI tract, abdominal cavity, and retroperitoneum were retrieved from consult and surgical pathology archives, including 54 GISTs (8 KIT-negative; 13 desmin-positive), 17 GI leiomyosarcomas, 11 GI mural leiomyomas, 13 leiomyomas of the muscularis mucosae, 12 gastric schwannomas, 15 inflammatory myofibroblastic tumors, 9 cases of mesenteric desmoid fibromatosis, 10 dedifferentiated liposarcomas, and 9 malignant peripheral nerve sheath tumors.

我们从会诊病例及活检病例中找出150例间叶性肿瘤（来自胃肠道、腹腔和后腹膜），其中包括54例GISTs（8例KIT阴性、13例desmin阳性）、17例GI道平滑肌肉瘤、11例GI道肌壁间平滑肌瘤、13例GI道粘膜肌层平滑肌瘤、12例胃的神经鞘瘤、15例炎性肌纤维母细胞瘤、9例肠系膜韧带样纤维瘤、10例去分化型脂肪肉瘤和9例恶性外周神经鞘膜瘤。

None of the GISTs, desmoid tumors, inflammatory myofibroblastic tumors, schwannomas, dedifferentiated liposarcomas, or malignant peripheral nerve sheath tumors showed cytoplasmic reactivity for smoothelin.

所有GISTs、韧带样纤维瘤、炎性肌纤维母细胞瘤、神经鞘瘤、去分化脂肪肉瘤和恶性外周神经鞘膜瘤病例的瘤细胞浆均不表达smoothelin。

The time of cornea diabrosis, metastases into cervical lymph nodes and lung, survival time and ratio of CD4+ and CD8+ T cells in peripheral blood were observed.RESULTS: The time of cornea diabrosis in GTVC group or GTV group was later than that in PBS group, but no statistical difference between GTVC group and GTV group. Metastatic incidence in cervical lymph nodes of GTVC group mice or GTV group mice was lower than that in PBS group 18 days after the model had been established. There was no statistical difference in metastatic incidence between GTV group and GTVC group.

将荷瘤模型鼠分为磷酸盐缓冲液（phosphatebuffered saline，PBS）治疗组，IL12基因修饰肿瘤细胞疫苗（gene modified tumor cell vaccine， GTV）治疗组及GTV 联合CpGODN治疗组；鼠荷瘤模型建立后的第3d及第10d分别在各组鼠结膜下注射30μL PBS，30μL（3.0×106个细胞）灭活的GTV及含20μg CpGODN灭活的GTV，进行角膜溃破时间、淋巴结和肺部转移瘤发生率、生存时间及外周血CD4＋和CD8＋T细胞比例的观测。

Objective To explore the effect of different anticoagulants on quantitative assays for HBV DNA using real-time PCR technique. Methods Peripheral blood samples were obtained from 40 patients infected with HBV. Serum and plasma samples from three kinds of anticoagulants such as heparin, trisodium citrate, and dipotassium EDTA were stored at -20℃.

目的 探讨用实时荧光定量PCR技术检测乙型肝炎病毒DNA拷贝时，不同抗凝剂对乙型肝炎病毒DNA定量检测的影响方法采集40例乙型肝炎患者血液，分别置于不含抗凝剂及含肝素钠、枸缘酸钠、EDTA-K2抗凝剂的无菌试管中，分离血清和血浆后于-20℃冰箱保存备用。

Methods peripheral blood samples were obtained from 40 patients infected with hbv. serum and plasma samples from three kinds of anticoagulants such as heparin, trisodium citrate, and dipotassium edta were stored at -20℃.

采集40例乙型肝炎患者血液，分别置于不含抗凝剂及含肝素钠、枸缘酸钠、edta-k2抗凝剂的无菌试管中，分离血清和血浆后于-20℃冰箱保存备用。

Objectives A rat model sciatic nerve crush injury was reproduced in rat, and behavioral changes, pain threshold, thermal withdrawal latency and the changes in c-fos expression in cornu dorsale medullae spinalis were examined, and the relationship between peripheral nerve regeneration and hyperpathia was explored.

目的 建立大鼠坐骨神经挤压模型，观察其神经恢复过程中行为学、机械痛阈值、热缩足反射潜伏期以及脊髓背角c-fos表达（对c-fos表达产物：Fos蛋白阳性的神经元进行计数）的改变，探讨外周神经损伤再生与痛觉过敏的关系。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。