查询词典 pavement cells

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

China's "Highway Engineering Technical Standards" will be divided into high-level road according to technical quality, sub-senior, middle and low-level four kinds, all kinds of road surface types are as follows: Senior Pavement - asphalt concrete pavement, cement concrete pavement, plant mix asphalt crushed stone pavement, stone pavement of rocks or neat; second high-level road surface - asphalt penetration-style pieces, gravel road, asphalt road debris, gravel road, asphalt road surface, Treatment, semi-neat stone pavement; middle road - broken, gravel (size distribution, or clay-bound) road surface, irregular stone pavement, other granular surface; low road - aggregates stabilized soil surface, and other local materials, reinforcement or improvement of soil surface.

中国《公路工程技术标准》将路面按技术品质分为高级、次高级、中级和低级四种，各种路面的面层类型如下：高级路面——沥青混凝土路面，水泥混凝土路面，厂拌沥青碎石路面，整齐石块或条石路面；次高级路面——沥青贯入式碎、砾石路面，路拌沥青碎、砾石路面，沥青表面处治路面，半整齐石块路面；中级路面——碎、砾石路面，不整齐石块路面，其他粒料路面；低级路面——粒料加固土路面，其他当地材料加固或改善土路面。

Neural stem cells have a strong self-renew mechanism and it can transform after a little break. Neural stem cells have a long term survival, which mean that it has more probability of wrong copy than mature cells. These cells are formed glioma stem cells in the end. The genes who adjust neural stem cells can express in glioma stem cells, which hold out glioma stem cells from neural stem cells. There is another presume that glioma stem cells come from differentiated cells. Through the gene break of these cells, they can obtain characteristics of stem cells, then form glioma stem cells.

神经干细胞具有很强的自我更新机制，获得较少突变即有可能恶性转化，而且干细胞存活时间较长，这意味着干细胞比成熟细胞发生细胞复制的错误几率更大，因外界环境的刺激而发生突变的机会更多，最终形成脑胶质瘤干细胞，同时调节神经干细胞增殖和自我更新的基因在脑胶质瘤的脑胶质瘤干细胞中也表达，这也是支持神经干细胞是脑胶质瘤干细胞来源的；也有推测认为它可能起源于已分化的细胞，由这些细胞突变发生去分化得来，并通过基因突变而获得了干细胞自我更新的特性，从而形成脑胶质瘤干细胞。

In recent years, the company has successfully developed some special production lines for manufacturing ordinary batteries, namely, D size, Size C, and Size AA. Now running in the workshop is another assembly line for carbon rod processing machinery. For the past decade, it has introduced most advanced manufacturing techniques and control techniques from abroad, and therefore it has managed to develop automated machines for producing alkaline batteries. The automatic assembly lines that it possesses are: Lines LR6 and LR03 for 200 cells per minute, 300 cells per minute, 400 cells per minute, 600 cells per minute and 1000 cells per minute, Lines LR20, LR14 and LR61 for 150 cells per minute and 300 cells per minute. Powder spraying conducting film, machines for negative electrode calamine cream, diaphragm machines with a speed of 30 cells per minute; negative electrode electric welding machines with a speed of 200-300 cells per minute; current collector assembling machines with a speed of 200-400 cells per minute; battery tray fillers with a speed of 200-400 cells per minute, insulating ring assembling machines with a speed of 300 cells per minute; electroscope machines, 4-cell furling machines with a speed of 300 cells per minute.

公司先后开发了普通电池生产线：一号、二号、五号电池流水线和普通碳棒加工机械，特别是近十年来公司吸收并消化了国外最先进的生产工艺、控制技术，研制开发了国内最先进的自动化碱性电池机械，有200只/分钟、300只/分钟、400只/分钟、600只/分钟、1000只/分钟的LR6、LR03电池自动化生产流水线；有150只/分钟、300只/分钟的LR20、LR14、LR61电池生产线；有正极拌粉系统喷导电膜设备、负极拌锌膏；有30只/分钟的卷隔膜套机，200-300只/分钟负极钉高速点焊机，200-400只/分钟的集电体高速组装机，200-400只/分钟的电池装盘机，300只/分钟的绝缘圈组装机，300只/分钟的验电机和四节缩机等。

The result showed that the surface architecture and ultrastructure of I.punctatus ' gill and secondary gill lamellae were similar to those of other teleosts. The surface of gill arches and gill rakers was covered with pavement cells which were characterized by circular microridges, and secreting-holes were found among cells on the surface of gill arches. The base part of gill rakers were bigger in terms of diameter. The pavement cells' surface of gill filaments' middle part was characterized by microridges with fine lateral protuberances on both sides which were either fused or interruped, while the microridges disappeared on the pavement cells' surface on both top and base parts of gill filaments, forming dense granulated protuberances. The height and interlamellar space of secondary gill lamellae in I.punctatus were relatively higher and bigger, respectively.

结果表明，斑点叉尾鮰鳃的表面结构和微细结构与其他硬骨鱼类基本相似，鳃弓和鳃耙表面被具环形微嵴的扁平上皮细胞所覆盖，鳃弓表面细胞之间有孔洞；鳃耙基部较粗壮；中部鳃丝上皮细胞表面的微嵴两侧有细小的横突，有些微嵴出现融合或间断；端部和基部鳃丝上皮细胞表面的微嵴消失，形成密集颗粒状突起；鳃小片的高度和片间距较大。

The feasibility of neural network application to asphalt concrete pavement performance forecast is set forth. Three main environmental factors-traffic, rainfall, temperature, which can lead to the direct change of pavement performance are used as the input layer of neural network. The performance indexes of asphaltum concrete pavement, i.e. dilapidation rate, smoothness, deflection, friction coefficient are used as output layer. The relation between environment factor and pavement performance is acquired by introducing the module of BP Neural Network in MATLAB.

阐述了应用BP神经网络进行沥青混凝土路面使用性能预测的可行性，以环境因素，即交通量、降雨量、温度等3个直接使路面性能发生变化的主要因素作为BP神经网络的输入层，以沥青混凝土路面的破损率、平整度、弯沉值、摩擦系数等4项性能指标作为输出层，采用MATLAB语言中的BP神经网络模块进行自学习，得出环境因素与路面使用性能之间的关系。

There are great differences between the axle load used in the pavement design and the load moving at the actual pavement. The difference affects the degree of pavement damage due to axle load and the pavement life.

路面上实际行驶的车辆其荷载的大小与路面设计时所采用的理论值有一定的偏差，偏差的大小直接影响车辆对路面的损害程度和路面的使用寿命。

The paper through the mechanism which produces to the asphalt road surface water harm carries on the induction to summarize, as well as the different type bituminous mixture water stability has carried on a series of contrasts experiment to several kind of different aggregates and the asphalt adherency, has analyzed the aggregate and the asphalt adherency and the bituminous mixture water stability; Meanwhile moves the water action mechanism take the pavement structure in as the key point, in the basis room experiments to the design half rigid base bituminous mixture test sample carries on washes out the experiment, the observation test sample and washes out when the number of times increase along with the time variation the basic unit wash load situation, has analyzed the water to the bituminous pavement various structures level damage rule; To immerses under the condition using the ANASYS finite element software the bituminous pavement structure level the stress to carry on the analysis computation, and analyzed the bituminous pavement according to the computed result to produce the unboiled water harm the rule, further to understand the structure level produced the unboiled water harm the rule.

本论文通过对沥青路面水损害产生的机理进行归纳总结，对几种不同集料和沥青的粘附性以及采用不同抗剥落措施的沥青混合料的水稳性进行了一系列对比试验，分析了集料与沥青的粘附性及沥青混合料的水稳定性，并对水稳性试验进行了评价；同时以路面结构中的动水作用机理为重点，依据室内试验对设计的半刚性基层沥青混合料组合试件进行冲刷试验，观察试件随时间变化和冲刷次数增加时基层的冲刷量情况，分析了水对沥青路面结构层的损坏规律；利用ANSYS有限元软件对浸水前后沥青路面层底应力进行分析计算，并根据计算结果分析了沥青路面产生水损害的规律，进一步了解结构层产生水损害的规律。

According to many years asphalt pavement construction experience of the author and combining with Technical Specification for Construction of Asphalt Pavement,this passage makes brief introduction to the two asphalt pavement construction ways,e.g.using covering cloth and using hot-mixed asphaltic concrete so as to provide reference for people who work on asphalt pavement project.

文章根据作者多年来从事沥青路面施工的经验，结合《沥青路面施工技术规范》，对撒布法沥青路面面层和热拌沥青混合料沥青路面面层施工分别作了简要介绍，以便他人从事沥青路面施工时作参考。

Thermal respone of building surfaces, grass land, asphaltic pavement and cement concrete pavement by solar radiation was measured using infrared camera. The results showed that the temperature of asphaltic pavement, cement concrete pavement and the building surfaces are high, middle and low, respectively at the same day.

该文利用红外热像仪等设备研究了建筑物表面、绿地、柏油路面、水泥砖地面等城市地表覆盖物对太阳辐射的热反应，指出在同一天中，柏油路面温度最高，水泥砖和建筑物表面次之，绿地温度最低。

His female bullfighter Lydia gradually Health love, but she was in a vegetative state after the Bulls Zhuangcheng away from her, just because Lydia's ex-boyfriend showed more affectionate than he is like love.

他对女斗牛士莉迪亚渐生爱意，却在她被公牛撞成植物人后离她而去，仅仅因为莉迪亚的前男友表现出好似比他更深情的爱。

I know of none which are not easy to answer, but what I am trying to do now is only by a series of hints to throw some light on the narrow path of legal doctrine, and upon two pitfalls which, as it seems to me, lie perilously near to it.

我不认为有什么难以回答的问题，但我现在要做的仅仅是通过一系列线索，来给法律理论的狭窄路径以及在我看来离它近得危险的两个陷阱提供一些启示。