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7Kb of these isolates were amplified by using RT-PCR The amplified DNA were then cloned and sequenced The analysis of the sequence data further confirmed that IBV was the pathogenic agent of the infectious disease associated with proventricular lesion

利用扩增片段两端的EcoRⅠ和BamHⅠ位点将扩增片段克隆进pBluescripⅡKS载体,转化大肠杆菌JM109,挑选白色菌落并用PCR方法对插入片段进行鉴定。用双脱氧终止法对扩增的片段进行测序并与GenBank公开的序列进行比较分析。结果进一步证实IBV是导致此种野外发生的腺胃相关疾病的病原。

In order to fully know the alteration of microbial community in chick, the influence of different kinds of pathogenic microorganism on chick, and the difference of intestinal flora, in this paper analysed the structure of intestinal flora of pullorum disease group, newcastle disease group and healthy group by ERIC-PCR based fingerprint on Molecular Ecology.

中文摘要:为了全面掌握鸡肠道微生物群落结构变化,人工感染病原微生物对雏鸡肠道菌群结构的影响以及不同类型病原微生物对菌群的影响是否存在差异,本文应用ERIC-PCR指纹图谱技术,对鸡白痢、新城疫感染雏鸡以及健康鸡的肠道菌群结构特征进行了分子生态学研究。

Objective To review the effect of the inflammatory factor and adherence factor on pyretic syndrome tumor by the treatment of QingreXiaotansanjie, through establish the S180 tumor model of TCM pyretic syndrome by alcohol, and to approach the mechanism of action about the pathogenic heat affect tumor'growth and development.

目的通过利用乙醇干预建立热邪干预下S180肉瘤模型,观察清热消痰散结法对热邪干预下S180肉瘤在粘附分子、炎性因子及血管内皮生长因子等方面的影响,探讨热邪对肿瘤发生发展的作用机制。

Objective To review the effect of the inflammatory factor and adherence factor on pyretic syndrome tumor by the treatment of Qingre Xiaotansanjie , through establish the S180 tumor model of TCM pyretic syndrome by alcohol, and to approach the mechanism of action about the pathogenic heat affect tumor'growth and development.

目的通过利用乙醇干预建立热邪干预下S180肉瘤模型,观察清热消痰散结法对热邪干预下S180肉瘤在粘附分子、炎性因子及血管内皮生长因子等方面的影响,探讨热邪对肿瘤发生发展的作用机制。

Methods:establishing s180 tumor model,and establishing the model of pyretic syndrome by alcohol. the centrifuged blood was detected pge-2 by the method of radioimmunity,and observed the condition of hot syndrome.to detect il-8 in tumor tissue of s180 mouse be interfered in hot pathogenic factor by method of abc-elisa.

利用腋下种植s180肉瘤细胞株建立肿瘤模型,并用灌胃乙醇的方法建立肿瘤热证模型,利用放射免疫方法检测s180小鼠血清中pge-2的水平,采用双抗体夹心abc-elisa法测定热邪干预s180小鼠肿瘤组织中il-8的含量。

The pharmacological mechanism is that it may reduce the"pathogenic pyretic factors"by means of"expelling heat to promote the production of body fluid", but it cannot eliminate the existent blood stasis (3) The blood stasis was stopped in QHR.

白虎汤能明显改善血浆比粘度、血沉、ET、TXB〓、PT等血瘀相关性实验指标,其机理可能是该方通过清热生津,减少血瘀形成的基本诱因&热毒&而发挥作用,但该方不能防止气分证微观血瘀的形成。

Studies on Pathogenic Species of Pythium from Cool-season Turfgrasses in Zhejiang Province;2. The results indicated that the main turfgrass diseases were brown patch, pythium and fuarium diseases.

对北京市海淀区部分草坪的病害种类调查表明:草坪的病害主要是由丝核菌属、腐霉菌属、镰刀菌属引起的褐斑病、腐霉枯萎病和镰刀枯萎病。

Conelusion:Missense mutation of Tyr486Asp in Exon 5 of UGT1A1 gene is the pathogenic factor of the CN Ⅱ case in the family and recessively inherited.

Tyr486Asp突变是本例CN—Ⅱ家系的致病性基因,该突变不影响UGT1A1基因启动子区苯巴比妥酸的应答元件,其遗传规律符合常染色体隐性遗传。

Missense mutation of Tyr486Asp in Exon 5 of UGT1A1 gene is the pathogenic factor of the CNⅡ case in the family and recessively inherited. The mutation has no influence on the response elements of phenibarbital in the promoter region.

Tyr486Asp突变是本例CN-Ⅱ家系的致病性基因,该突变不影响UGT1A1基因启动子区苯巴比妥酸的应答元件,其遗传规律符合常染色体隐性遗传。

The basic principles are the same, that is, to p revent the spread and cause the destruction of pathogenic microorganisms.

它们的基本原理是相同的,也就是要防止致病微生物的传播和消灭致病微生物。

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