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In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase activity and glutamic-aspartic aminotransferase activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9、59.6、 56.2、 54.7、 47.3、 38.2、 30.8、 23.8、 18.4、 16.8、14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.

本研究采用SDS-PAGE对大熊猫精浆蛋白电泳谱带特性进行分析，使用酶联免疫方法检测精浆血小板激活因子乙酰水解酶活性、化学比色法检测精浆谷草转氨酶活性，并初步探讨了这些物质与大熊猫精液质量的关系，结果显示：(1)大熊猫精浆SDS-PAGE电泳共分离得到12条蛋白谱带，其分子量分别为74.9kDa、59.6kDa、56.2kDa、54.7kDa、47.3kDa、38.2kDa、30.8kDa、23.8kDa、18.4kDa、16.8kDa、14.0kDa和13.4kDa，其中74.9kDa、59.6kDa、56.2kDa、47.3kDa、30.8kDa、13.4kDa谱带为所有个体大熊猫精浆样品所共有。

The chromosomal constitution of 200 advanced lines from 1R monosomic addition lines between the wheat cultivar Mianyang11 (MY11) and different rye lines R3, R12, and Baili-rye was studied by the improved C-banding and A-PAGE method. The result showed that 45 lines were identified as 1RS/1BL translocation lines congruously by the two methods. But a line (843-1-1), originated from an 1R addition line of wheat cultivar MY11 and rye line R12, was identified as 1RS/1BL translocation line by C-banding and genomic in situ hybridization,but the secalin block from chromosome arm 1RS of rye was absent in the A-PAGE pattern of its seed proteins.

结果发现，在鉴定的200个株系中，有45个株系经C-带和A-PAGE检测均一致地发现它们含有一对1RS /1BL易位染色体，而一个株系843-1-1，C-带鉴定、原位杂交结果均证明它含有一对1RS/1BL易位染色体，但A-PAGE醇溶蛋白图谱却不具有黑麦1RS染色体臂的黑麦碱特征带，而表达出既不同于黑麦碱又不同于亲本绵阳11的醇溶蛋白带型。

In the paper, a preliminary study was made to analyze the interaction between hemocyanin, which was purified from shrimp P. vannamei obtained from Shantou, and anti -human antibody. The methods of Sephadex G-75 column chromatography, PAGE, Dot-ELISA, SDS-PAGE and Western-blotting were used.

以采自汕头的南美白对虾血清为研究对象，运用Sephdex-75柱层析、PAGE、Dot-ELISA、SDS-PAGE和Western-blotting等方法，探索血蓝蛋白与抗人IgG之间的相互作用。

Researches have been carried out during 1989￣1995 on variety identification of maize, sorghum, tomato, sorghum.tomato,water melon, cucumber, pepper, Chinese cabbage, radish, lagenaria siceraria and melon by PAGE method of seed protein and EST, POD, AES, AOH isozymes.

在1989～1995年期间，以玉米、高粱、番茄、西瓜、黄瓜、辣椒、白菜、萝卜、瓠瓜、甜瓜等10种作物为试材，研究了EST，POD，AES和ADH4种同工酶的PAGE电泳和IEF，SDS－PAGE，PAGE蛋白质电泳谱带法在品种纯度鉴定上的应用。

You can modify the engine power from 0 to 100% by pressing either the "Page Up" or "Page Down" key.

你能更改发动机的功率从0到100％通过按"Page Up"或"Page Down"键。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

At present each basically search engine to count limitation to be respectively to the word of Page Title: Note: The unit is a Chinese character, be not English letter.

Page Title中的要害词：为优化考虑，Page Title要注重有机地嵌入内容相关的要害词，而这些要害词的重要性则与位置有关：即出现在Page Title中最左边的要害词最重要，第二个次之，等等。

The content ofone liver protein band (R_f=0.79) at 1℃was significantly higher than at room temperature (P＜0.05).On the 2-D native-PAGE/SDS-PAGE gel, the content of P spot (MW=51kDa, R_1=0.47on the 1D native PAGE gel) at 1℃was significantly higher than at room temperature (P＜0.05), and the content of N spot (MW=65 kDa, R_f=0,94 on the 1D native PAGE gel) at roomtemperature was significantly higher than at 1℃. These protein spots may be related with the adaptation to the low temperature of the Heilongjiang wood frog.

黑龙江林蛙肝脏蛋白电泳显示，胶浓度6％的肝脏PAGE图谱分辨率高、谱带多，可分辨出15～19条谱带，其中1℃组R_f=0.79蛋白含量比室温组显著增加(P＜0.05)。2-D native-PAGE／SDS-PAGE电泳时，胶浓度为12％时分离效果较好，蛋白斑点较多。1℃组中P蛋白斑点(MW=51kDa，一向PAGE的R_f=0.47)显著性上调(P＜0.05)，N蛋白斑点(MW=65kDa，一向PAGE的R_f=0.94)显著性下调(P＜0.05)，推测这两种蛋白可能与黑龙江林蛙在低温环境下的适应性变化有关。

The protein profiles of each microsporidia are complex.There are more than 30 bands and 5 major bands on the SDS-PAGE profiles of each microsporidia.

对总蛋白进行了酸性PAGE、碱性PAGE、SDS-PAGE和氨基酸组成分析，并对SDS-PAGE结果进行了薄层扫描分析。

Modern DRAMs support two page strategies: close page and open page.

现代DRAM支持两种page策略：close page和open page。

But in the course of internationalization, they meet with misunderstanding and puzzlement.

许多企业已经意识到了这一点，但在国际化的进程中，仍存在一些误区与困惑。

Inorder toaccomplish this goal as quickly as possible, we'll beteamingup with anexperienced group of modelers, skinners, and animatorswhosenames willbe announced in the coming weeks.

为了尽快实现这个目标，我们在未来数周内将公布与一些有经验的模型、皮肤、动画制作小组合作。